Cargando…
Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy
PURPOSE: To assess vitreous levels of inflammatory cytokines and neurotrophins (NTs) in diabetic retinopathy (DR) and elucidate their potential roles. METHODS: A prospective study was performed on 50 vitreous samples obtained from patients with DR (n = 22) and the nondiabetic controls (n = 28). All...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667399/ https://www.ncbi.nlm.nih.gov/pubmed/29084332 http://dx.doi.org/10.1167/iovs.17-21973 |
_version_ | 1783275477983559680 |
---|---|
author | Boss, Joseph D. Singh, Pawan Kumar Pandya, Hemang K. Tosi, Joaquin Kim, Chaesik Tewari, Asheesh Juzych, Mark S. Abrams, Gary W. Kumar, Ashok |
author_facet | Boss, Joseph D. Singh, Pawan Kumar Pandya, Hemang K. Tosi, Joaquin Kim, Chaesik Tewari, Asheesh Juzych, Mark S. Abrams, Gary W. Kumar, Ashok |
author_sort | Boss, Joseph D. |
collection | PubMed |
description | PURPOSE: To assess vitreous levels of inflammatory cytokines and neurotrophins (NTs) in diabetic retinopathy (DR) and elucidate their potential roles. METHODS: A prospective study was performed on 50 vitreous samples obtained from patients with DR (n = 22) and the nondiabetic controls (n = 28). All patients were candidates for vitrectomy. Inflammatory cytokine and NT levels were determined with ELISA. Potential source and role of NTs was determined by using human retinal Müller glia and mouse photoreceptor cells and challenging them with TNF-α or IL-1β, followed by detection of NTs and cell death. RESULTS: Vitreous NT levels of all DR patients were significantly higher than those of nondiabetic controls (nerve growth factor [NGF, P = 0.0001], brain-derived neurotrophic factor [BDNF, P = 0.009], neurotrophin-3 [NT-3, P < 0.0001], neurotrophin-4 [NT-4, P = 0.0001], ciliary neurotrophic factor [CNTF, P = 0.0001], and glial cell–derived neurotrophic factor [GDNF, P = 0.008]). Similarly, the levels of inflammatory mediators IL-1β (P < 0.0001), IL-6 (P = 0.0005), IL-8 (P < 0.0001), and TNF-α (P < 0.0001) were also higher in eyes with DR. Interestingly, inflammatory cytokine and NT levels, particularly TNF-α (P < 0.05), IL-8 (P < 0.004), NT-3 (P = 0.012), NGF (P = 0.04), GDNF (P = 0.005), and CNTF (P = 0.002), were higher in eyes with nonproliferative diabetic retinopathy (NPDR) than in eyes with active proliferative diabetic retinopathy (PDR). Cytokine stimulation of Müller glia resulted in production of NTs, and GDNF treatment reduced photoreceptor cell death in response to inflammation and oxidative stress. CONCLUSIONS: Together, our study demonstrated that patients with DR have higher levels of both inflammatory cytokines and NTs in their vitreous. Müller glia could be the potential source of NTs under inflammatory conditions to exert neuroprotection. |
format | Online Article Text |
id | pubmed-5667399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56673992017-11-03 Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy Boss, Joseph D. Singh, Pawan Kumar Pandya, Hemang K. Tosi, Joaquin Kim, Chaesik Tewari, Asheesh Juzych, Mark S. Abrams, Gary W. Kumar, Ashok Invest Ophthalmol Vis Sci Retina PURPOSE: To assess vitreous levels of inflammatory cytokines and neurotrophins (NTs) in diabetic retinopathy (DR) and elucidate their potential roles. METHODS: A prospective study was performed on 50 vitreous samples obtained from patients with DR (n = 22) and the nondiabetic controls (n = 28). All patients were candidates for vitrectomy. Inflammatory cytokine and NT levels were determined with ELISA. Potential source and role of NTs was determined by using human retinal Müller glia and mouse photoreceptor cells and challenging them with TNF-α or IL-1β, followed by detection of NTs and cell death. RESULTS: Vitreous NT levels of all DR patients were significantly higher than those of nondiabetic controls (nerve growth factor [NGF, P = 0.0001], brain-derived neurotrophic factor [BDNF, P = 0.009], neurotrophin-3 [NT-3, P < 0.0001], neurotrophin-4 [NT-4, P = 0.0001], ciliary neurotrophic factor [CNTF, P = 0.0001], and glial cell–derived neurotrophic factor [GDNF, P = 0.008]). Similarly, the levels of inflammatory mediators IL-1β (P < 0.0001), IL-6 (P = 0.0005), IL-8 (P < 0.0001), and TNF-α (P < 0.0001) were also higher in eyes with DR. Interestingly, inflammatory cytokine and NT levels, particularly TNF-α (P < 0.05), IL-8 (P < 0.004), NT-3 (P = 0.012), NGF (P = 0.04), GDNF (P = 0.005), and CNTF (P = 0.002), were higher in eyes with nonproliferative diabetic retinopathy (NPDR) than in eyes with active proliferative diabetic retinopathy (PDR). Cytokine stimulation of Müller glia resulted in production of NTs, and GDNF treatment reduced photoreceptor cell death in response to inflammation and oxidative stress. CONCLUSIONS: Together, our study demonstrated that patients with DR have higher levels of both inflammatory cytokines and NTs in their vitreous. Müller glia could be the potential source of NTs under inflammatory conditions to exert neuroprotection. The Association for Research in Vision and Ophthalmology 2017-11 /pmc/articles/PMC5667399/ /pubmed/29084332 http://dx.doi.org/10.1167/iovs.17-21973 Text en Copyright 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Retina Boss, Joseph D. Singh, Pawan Kumar Pandya, Hemang K. Tosi, Joaquin Kim, Chaesik Tewari, Asheesh Juzych, Mark S. Abrams, Gary W. Kumar, Ashok Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy |
title | Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy |
title_full | Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy |
title_fullStr | Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy |
title_full_unstemmed | Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy |
title_short | Assessment of Neurotrophins and Inflammatory Mediators in Vitreous of Patients With Diabetic Retinopathy |
title_sort | assessment of neurotrophins and inflammatory mediators in vitreous of patients with diabetic retinopathy |
topic | Retina |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667399/ https://www.ncbi.nlm.nih.gov/pubmed/29084332 http://dx.doi.org/10.1167/iovs.17-21973 |
work_keys_str_mv | AT bossjosephd assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT singhpawankumar assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT pandyahemangk assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT tosijoaquin assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT kimchaesik assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT tewariasheesh assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT juzychmarks assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT abramsgaryw assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy AT kumarashok assessmentofneurotrophinsandinflammatorymediatorsinvitreousofpatientswithdiabeticretinopathy |