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Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
Personalized molecular profiling has an established role in selection of treatment for metastatic disease; however, its role in improving radiosensitivity and functional imaging has not been evaluated. In the current study, we examined molecular profiling as a tool for designing personalized targete...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667418/ https://www.ncbi.nlm.nih.gov/pubmed/29109791 http://dx.doi.org/10.7150/thno.19615 |
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author | Hazkani, Inbal Motiei, Menachem Betzer, Oshra Sadan, Tamar Bragilovski, Dimitri Lubimov, Leon Mizrachi, Aviram Hadar, Tuvia Levi, Mattan Ben-Aharon, Irit Haviv, Izhack Popovtzer, Rachela Popovtzer, Aron |
author_facet | Hazkani, Inbal Motiei, Menachem Betzer, Oshra Sadan, Tamar Bragilovski, Dimitri Lubimov, Leon Mizrachi, Aviram Hadar, Tuvia Levi, Mattan Ben-Aharon, Irit Haviv, Izhack Popovtzer, Rachela Popovtzer, Aron |
author_sort | Hazkani, Inbal |
collection | PubMed |
description | Personalized molecular profiling has an established role in selection of treatment for metastatic disease; however, its role in improving radiosensitivity and functional imaging has not been evaluated. In the current study, we examined molecular profiling as a tool for designing personalized targeted gold nanoparticles (GNP) to serve as dual-modal tumor radiosensitizers and functional imaging enhancers. To this end, molecular profiling of a patient's salivary gland adenoid cystic carcinoma (ACC) was performed, and anaplastic lymphoma kinase (ALK) mutation was detected. The extracted tumor was subcutaneously injected into mice, which were then treated either with radiation, the specific ALK inhibitor crizotinib, or a combination of therapies. One of these combinations, namely, ALK-targeted GNP (via crizotinib coating), was found to enhance radiation treatment, as demonstrated by a significant decrease in tumor volume over 24 days. In parallel, ALK-targeted GNP substantially augmented tumor visualization via computed tomography. The mechanism of radiosensitivity enhancement was mostly related to a diminished cell repair mechanism in tumors, as demonstrated by proliferating cell nuclear antigen staining. These findings indicate that personalized molecular profiling is an effective technique for enhancing cancer theranostics. |
format | Online Article Text |
id | pubmed-5667418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-56674182017-11-06 Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma Hazkani, Inbal Motiei, Menachem Betzer, Oshra Sadan, Tamar Bragilovski, Dimitri Lubimov, Leon Mizrachi, Aviram Hadar, Tuvia Levi, Mattan Ben-Aharon, Irit Haviv, Izhack Popovtzer, Rachela Popovtzer, Aron Theranostics Research Paper Personalized molecular profiling has an established role in selection of treatment for metastatic disease; however, its role in improving radiosensitivity and functional imaging has not been evaluated. In the current study, we examined molecular profiling as a tool for designing personalized targeted gold nanoparticles (GNP) to serve as dual-modal tumor radiosensitizers and functional imaging enhancers. To this end, molecular profiling of a patient's salivary gland adenoid cystic carcinoma (ACC) was performed, and anaplastic lymphoma kinase (ALK) mutation was detected. The extracted tumor was subcutaneously injected into mice, which were then treated either with radiation, the specific ALK inhibitor crizotinib, or a combination of therapies. One of these combinations, namely, ALK-targeted GNP (via crizotinib coating), was found to enhance radiation treatment, as demonstrated by a significant decrease in tumor volume over 24 days. In parallel, ALK-targeted GNP substantially augmented tumor visualization via computed tomography. The mechanism of radiosensitivity enhancement was mostly related to a diminished cell repair mechanism in tumors, as demonstrated by proliferating cell nuclear antigen staining. These findings indicate that personalized molecular profiling is an effective technique for enhancing cancer theranostics. Ivyspring International Publisher 2017-09-14 /pmc/articles/PMC5667418/ /pubmed/29109791 http://dx.doi.org/10.7150/thno.19615 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Hazkani, Inbal Motiei, Menachem Betzer, Oshra Sadan, Tamar Bragilovski, Dimitri Lubimov, Leon Mizrachi, Aviram Hadar, Tuvia Levi, Mattan Ben-Aharon, Irit Haviv, Izhack Popovtzer, Rachela Popovtzer, Aron Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
title | Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
title_full | Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
title_fullStr | Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
title_full_unstemmed | Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
title_short | Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
title_sort | can molecular profiling enhance radiotherapy? impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667418/ https://www.ncbi.nlm.nih.gov/pubmed/29109791 http://dx.doi.org/10.7150/thno.19615 |
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