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Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma

Personalized molecular profiling has an established role in selection of treatment for metastatic disease; however, its role in improving radiosensitivity and functional imaging has not been evaluated. In the current study, we examined molecular profiling as a tool for designing personalized targete...

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Autores principales: Hazkani, Inbal, Motiei, Menachem, Betzer, Oshra, Sadan, Tamar, Bragilovski, Dimitri, Lubimov, Leon, Mizrachi, Aviram, Hadar, Tuvia, Levi, Mattan, Ben-Aharon, Irit, Haviv, Izhack, Popovtzer, Rachela, Popovtzer, Aron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667418/
https://www.ncbi.nlm.nih.gov/pubmed/29109791
http://dx.doi.org/10.7150/thno.19615
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author Hazkani, Inbal
Motiei, Menachem
Betzer, Oshra
Sadan, Tamar
Bragilovski, Dimitri
Lubimov, Leon
Mizrachi, Aviram
Hadar, Tuvia
Levi, Mattan
Ben-Aharon, Irit
Haviv, Izhack
Popovtzer, Rachela
Popovtzer, Aron
author_facet Hazkani, Inbal
Motiei, Menachem
Betzer, Oshra
Sadan, Tamar
Bragilovski, Dimitri
Lubimov, Leon
Mizrachi, Aviram
Hadar, Tuvia
Levi, Mattan
Ben-Aharon, Irit
Haviv, Izhack
Popovtzer, Rachela
Popovtzer, Aron
author_sort Hazkani, Inbal
collection PubMed
description Personalized molecular profiling has an established role in selection of treatment for metastatic disease; however, its role in improving radiosensitivity and functional imaging has not been evaluated. In the current study, we examined molecular profiling as a tool for designing personalized targeted gold nanoparticles (GNP) to serve as dual-modal tumor radiosensitizers and functional imaging enhancers. To this end, molecular profiling of a patient's salivary gland adenoid cystic carcinoma (ACC) was performed, and anaplastic lymphoma kinase (ALK) mutation was detected. The extracted tumor was subcutaneously injected into mice, which were then treated either with radiation, the specific ALK inhibitor crizotinib, or a combination of therapies. One of these combinations, namely, ALK-targeted GNP (via crizotinib coating), was found to enhance radiation treatment, as demonstrated by a significant decrease in tumor volume over 24 days. In parallel, ALK-targeted GNP substantially augmented tumor visualization via computed tomography. The mechanism of radiosensitivity enhancement was mostly related to a diminished cell repair mechanism in tumors, as demonstrated by proliferating cell nuclear antigen staining. These findings indicate that personalized molecular profiling is an effective technique for enhancing cancer theranostics.
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spelling pubmed-56674182017-11-06 Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma Hazkani, Inbal Motiei, Menachem Betzer, Oshra Sadan, Tamar Bragilovski, Dimitri Lubimov, Leon Mizrachi, Aviram Hadar, Tuvia Levi, Mattan Ben-Aharon, Irit Haviv, Izhack Popovtzer, Rachela Popovtzer, Aron Theranostics Research Paper Personalized molecular profiling has an established role in selection of treatment for metastatic disease; however, its role in improving radiosensitivity and functional imaging has not been evaluated. In the current study, we examined molecular profiling as a tool for designing personalized targeted gold nanoparticles (GNP) to serve as dual-modal tumor radiosensitizers and functional imaging enhancers. To this end, molecular profiling of a patient's salivary gland adenoid cystic carcinoma (ACC) was performed, and anaplastic lymphoma kinase (ALK) mutation was detected. The extracted tumor was subcutaneously injected into mice, which were then treated either with radiation, the specific ALK inhibitor crizotinib, or a combination of therapies. One of these combinations, namely, ALK-targeted GNP (via crizotinib coating), was found to enhance radiation treatment, as demonstrated by a significant decrease in tumor volume over 24 days. In parallel, ALK-targeted GNP substantially augmented tumor visualization via computed tomography. The mechanism of radiosensitivity enhancement was mostly related to a diminished cell repair mechanism in tumors, as demonstrated by proliferating cell nuclear antigen staining. These findings indicate that personalized molecular profiling is an effective technique for enhancing cancer theranostics. Ivyspring International Publisher 2017-09-14 /pmc/articles/PMC5667418/ /pubmed/29109791 http://dx.doi.org/10.7150/thno.19615 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Hazkani, Inbal
Motiei, Menachem
Betzer, Oshra
Sadan, Tamar
Bragilovski, Dimitri
Lubimov, Leon
Mizrachi, Aviram
Hadar, Tuvia
Levi, Mattan
Ben-Aharon, Irit
Haviv, Izhack
Popovtzer, Rachela
Popovtzer, Aron
Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
title Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
title_full Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
title_fullStr Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
title_full_unstemmed Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
title_short Can molecular profiling enhance radiotherapy? Impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
title_sort can molecular profiling enhance radiotherapy? impact of personalized targeted gold nanoparticles on radiosensitivity and imaging of adenoid cystic carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667418/
https://www.ncbi.nlm.nih.gov/pubmed/29109791
http://dx.doi.org/10.7150/thno.19615
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