Cargando…
Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells
Interferonε (IFNε) is a unique type I IFN that has distinct functions from IFNα/β. IFNε is constitutively expressed at mucosal tissues, including the female genital mucosa, and is reported to be modulated by estrogen and seminal plasma. However, its regulation by cytokines, including TNFα, IL-1β, IL...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667464/ https://www.ncbi.nlm.nih.gov/pubmed/29118969 http://dx.doi.org/10.1186/s13578-017-0185-z |
_version_ | 1783275491495510016 |
---|---|
author | Couret, Jennifer Tasker, Carley Kim, Jaeha Sihvonen, Tiina Fruitwala, Saahil Quayle, Alison J. Lespinasse, Pierre Heller, Debra S. Chang, Theresa L. |
author_facet | Couret, Jennifer Tasker, Carley Kim, Jaeha Sihvonen, Tiina Fruitwala, Saahil Quayle, Alison J. Lespinasse, Pierre Heller, Debra S. Chang, Theresa L. |
author_sort | Couret, Jennifer |
collection | PubMed |
description | Interferonε (IFNε) is a unique type I IFN that has distinct functions from IFNα/β. IFNε is constitutively expressed at mucosal tissues, including the female genital mucosa, and is reported to be modulated by estrogen and seminal plasma. However, its regulation by cytokines, including TNFα, IL-1β, IL-6, IL-8, IL-17, IL-22 and IFNα, which are commonly present in the female genital mucosa, is not well documented in freshly isolated primary cervical cells from tissues. We determined the effect of these cytokines on gene expression of type I IFNs in an immortalized endocervical epithelial cell line (A2EN) and in primary cervical epithelial cells. Several pro-inflammatory cytokines were found to induce IFNε, and TNFα induced the strongest response in both cell types. Pretreatment of cells with the IκB inhibitor, which blocks the NF-κB pathway, suppressed TNFα-mediated IFNε gene induction and promoter activation. Expression of IFNα, IFNβ, and IFNε was differentially regulated in response to various cytokines. Taken together, our results show that regulation of these IFNs depends on cell type, cytokine concentration, and incubation time, highlighting the complexity of the cytokine network in the cervical epithelium. |
format | Online Article Text |
id | pubmed-5667464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56674642017-11-08 Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells Couret, Jennifer Tasker, Carley Kim, Jaeha Sihvonen, Tiina Fruitwala, Saahil Quayle, Alison J. Lespinasse, Pierre Heller, Debra S. Chang, Theresa L. Cell Biosci Letter to the Editor Interferonε (IFNε) is a unique type I IFN that has distinct functions from IFNα/β. IFNε is constitutively expressed at mucosal tissues, including the female genital mucosa, and is reported to be modulated by estrogen and seminal plasma. However, its regulation by cytokines, including TNFα, IL-1β, IL-6, IL-8, IL-17, IL-22 and IFNα, which are commonly present in the female genital mucosa, is not well documented in freshly isolated primary cervical cells from tissues. We determined the effect of these cytokines on gene expression of type I IFNs in an immortalized endocervical epithelial cell line (A2EN) and in primary cervical epithelial cells. Several pro-inflammatory cytokines were found to induce IFNε, and TNFα induced the strongest response in both cell types. Pretreatment of cells with the IκB inhibitor, which blocks the NF-κB pathway, suppressed TNFα-mediated IFNε gene induction and promoter activation. Expression of IFNα, IFNβ, and IFNε was differentially regulated in response to various cytokines. Taken together, our results show that regulation of these IFNs depends on cell type, cytokine concentration, and incubation time, highlighting the complexity of the cytokine network in the cervical epithelium. BioMed Central 2017-11-02 /pmc/articles/PMC5667464/ /pubmed/29118969 http://dx.doi.org/10.1186/s13578-017-0185-z Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Letter to the Editor Couret, Jennifer Tasker, Carley Kim, Jaeha Sihvonen, Tiina Fruitwala, Saahil Quayle, Alison J. Lespinasse, Pierre Heller, Debra S. Chang, Theresa L. Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells |
title | Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells |
title_full | Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells |
title_fullStr | Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells |
title_full_unstemmed | Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells |
title_short | Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells |
title_sort | differential regulation of ifnα, ifnβ and ifnε gene expression in human cervical epithelial cells |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667464/ https://www.ncbi.nlm.nih.gov/pubmed/29118969 http://dx.doi.org/10.1186/s13578-017-0185-z |
work_keys_str_mv | AT couretjennifer differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT taskercarley differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT kimjaeha differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT sihvonentiina differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT fruitwalasaahil differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT quaylealisonj differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT lespinassepierre differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT hellerdebras differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells AT changtheresal differentialregulationofifnaifnbandifnegeneexpressioninhumancervicalepithelialcells |