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Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target
BACKGROUND: SLC38A1/SNAT1 has been found to play an essential role in human development, but its role in osteosarcoma (OS) has yet to be evaluated. The purpose of this study was to assess the expression of SLC38A1/SNAT1 in patients with OS, and further investigate the mechanisms by which it affects...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668009/ https://www.ncbi.nlm.nih.gov/pubmed/29108276 http://dx.doi.org/10.18632/oncotarget.20693 |
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author | Wang, Miaomiao Liu, Ying Fang, Wenzheng Liu, Ke Jiao, Xiaodong Wang, Zhan Wang, Jiejun Zang, Yuan-Sheng |
author_facet | Wang, Miaomiao Liu, Ying Fang, Wenzheng Liu, Ke Jiao, Xiaodong Wang, Zhan Wang, Jiejun Zang, Yuan-Sheng |
author_sort | Wang, Miaomiao |
collection | PubMed |
description | BACKGROUND: SLC38A1/SNAT1 has been found to play an essential role in human development, but its role in osteosarcoma (OS) has yet to be evaluated. The purpose of this study was to assess the expression of SLC38A1/SNAT1 in patients with OS, and further investigate the mechanisms by which it affects tumor growth and metastasis. METHODS: Tissue microarray blocks and immunohistochemical studies were carried out to assess the expression of SNAT1 in 165 OS specimens. Its correlation with clinicopathological characteristics was then analyzed. The function of SNAT1 in OS cells was investigated by silencing SNAT1 using SNAT1-shRNA in vitro and in vivo. RESULTS: SNAT1 was highly expressed in 85% OS and significantly closely associated with pulmonary metastasis. Patients with high SNAT1 expression survived for shorter periods than those with low SNAT1 expression. Suppression of endogenous SNAT1 led to inhibition of cell proliferation, cell colony formation, and cell migration in vitro, and retarded tumor growth in xenograft models. Silencing SNAT1 reduced expression of MMP9, vimentin, fibronectin, p-Akt, p-mTOR, and VEGF. CONCLUSIONS: Our results indicated that increased expression of SNAT1 is a common event in OS. SNAT1 played an essential role in the development and progression of osteosarcoma, which may serve as a prognostic and therapeutic marker of OS. |
format | Online Article Text |
id | pubmed-5668009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56680092017-11-04 Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target Wang, Miaomiao Liu, Ying Fang, Wenzheng Liu, Ke Jiao, Xiaodong Wang, Zhan Wang, Jiejun Zang, Yuan-Sheng Oncotarget Research Paper BACKGROUND: SLC38A1/SNAT1 has been found to play an essential role in human development, but its role in osteosarcoma (OS) has yet to be evaluated. The purpose of this study was to assess the expression of SLC38A1/SNAT1 in patients with OS, and further investigate the mechanisms by which it affects tumor growth and metastasis. METHODS: Tissue microarray blocks and immunohistochemical studies were carried out to assess the expression of SNAT1 in 165 OS specimens. Its correlation with clinicopathological characteristics was then analyzed. The function of SNAT1 in OS cells was investigated by silencing SNAT1 using SNAT1-shRNA in vitro and in vivo. RESULTS: SNAT1 was highly expressed in 85% OS and significantly closely associated with pulmonary metastasis. Patients with high SNAT1 expression survived for shorter periods than those with low SNAT1 expression. Suppression of endogenous SNAT1 led to inhibition of cell proliferation, cell colony formation, and cell migration in vitro, and retarded tumor growth in xenograft models. Silencing SNAT1 reduced expression of MMP9, vimentin, fibronectin, p-Akt, p-mTOR, and VEGF. CONCLUSIONS: Our results indicated that increased expression of SNAT1 is a common event in OS. SNAT1 played an essential role in the development and progression of osteosarcoma, which may serve as a prognostic and therapeutic marker of OS. Impact Journals LLC 2017-09-05 /pmc/articles/PMC5668009/ /pubmed/29108276 http://dx.doi.org/10.18632/oncotarget.20693 Text en Copyright: © 2017 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Miaomiao Liu, Ying Fang, Wenzheng Liu, Ke Jiao, Xiaodong Wang, Zhan Wang, Jiejun Zang, Yuan-Sheng Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target |
title | Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target |
title_full | Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target |
title_fullStr | Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target |
title_full_unstemmed | Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target |
title_short | Increased SNAT1 is a marker of human osteosarcoma and potential therapeutic target |
title_sort | increased snat1 is a marker of human osteosarcoma and potential therapeutic target |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668009/ https://www.ncbi.nlm.nih.gov/pubmed/29108276 http://dx.doi.org/10.18632/oncotarget.20693 |
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