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Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer

The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this imp...

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Autores principales: Goto, Taichiro, Hirotsu, Yosuke, Nakagomi, Takahiro, Shikata, Daichi, Yokoyama, Yujiro, Amemiya, Kenji, Tsutsui, Toshiharu, Kakizaki, Yumiko, Oyama, Toshio, Mochizuki, Hitoshi, Miyashita, Yoshihiro, Omata, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668052/
https://www.ncbi.nlm.nih.gov/pubmed/29108319
http://dx.doi.org/10.18632/oncotarget.18159
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author Goto, Taichiro
Hirotsu, Yosuke
Nakagomi, Takahiro
Shikata, Daichi
Yokoyama, Yujiro
Amemiya, Kenji
Tsutsui, Toshiharu
Kakizaki, Yumiko
Oyama, Toshio
Mochizuki, Hitoshi
Miyashita, Yoshihiro
Omata, Masao
author_facet Goto, Taichiro
Hirotsu, Yosuke
Nakagomi, Takahiro
Shikata, Daichi
Yokoyama, Yujiro
Amemiya, Kenji
Tsutsui, Toshiharu
Kakizaki, Yumiko
Oyama, Toshio
Mochizuki, Hitoshi
Miyashita, Yoshihiro
Omata, Masao
author_sort Goto, Taichiro
collection PubMed
description The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this improves the accuracy of bronchoscopy for diagnosing lung cancer and to identify factors influencing sensitivity. The bronchoscopic sensitivity for diagnosing lung cancer was initially evaluated in 191 patients who underwent lobectomy after bronchoscopy at our hospital. Sputum, bronchial wash fluid, and resected lung cancer specimens were subsequently collected from 18 patients with peripheral small cell lung cancer for genomic analysis. DNA was extracted from formalin-fixed, paraffin-embedded surgical tissue specimens and the supernatant and cell fractions of sputum and bronchial wash fluid. Deep sequencing was performed using a lung cancer panel covering all exons of 53 lung cancer-related genes. The bronchoscopic sensitivity for diagnosing lung cancer at our hospital was 60.7%. Multivariate analysis revealed that this was influenced by tumor size and location, but not histological type or lymph node metastasis. The sensitivity was the highest for biopsy followed by curettage and bronchial wash specimens. DNA mutations homologous to those identified in the primary lesions were detected in the bronchial wash fluid of 10 patients (55.6%), while only 2 patients (11.1%) were diagnosed with lung cancer based on conventional cytological examinations. In conclusion, the addition of genomic analysis to routine pathological examinations improves the diagnostic accuracy of bronchoscopy.
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spelling pubmed-56680522017-11-04 Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer Goto, Taichiro Hirotsu, Yosuke Nakagomi, Takahiro Shikata, Daichi Yokoyama, Yujiro Amemiya, Kenji Tsutsui, Toshiharu Kakizaki, Yumiko Oyama, Toshio Mochizuki, Hitoshi Miyashita, Yoshihiro Omata, Masao Oncotarget Clinical Research Paper The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this improves the accuracy of bronchoscopy for diagnosing lung cancer and to identify factors influencing sensitivity. The bronchoscopic sensitivity for diagnosing lung cancer was initially evaluated in 191 patients who underwent lobectomy after bronchoscopy at our hospital. Sputum, bronchial wash fluid, and resected lung cancer specimens were subsequently collected from 18 patients with peripheral small cell lung cancer for genomic analysis. DNA was extracted from formalin-fixed, paraffin-embedded surgical tissue specimens and the supernatant and cell fractions of sputum and bronchial wash fluid. Deep sequencing was performed using a lung cancer panel covering all exons of 53 lung cancer-related genes. The bronchoscopic sensitivity for diagnosing lung cancer at our hospital was 60.7%. Multivariate analysis revealed that this was influenced by tumor size and location, but not histological type or lymph node metastasis. The sensitivity was the highest for biopsy followed by curettage and bronchial wash specimens. DNA mutations homologous to those identified in the primary lesions were detected in the bronchial wash fluid of 10 patients (55.6%), while only 2 patients (11.1%) were diagnosed with lung cancer based on conventional cytological examinations. In conclusion, the addition of genomic analysis to routine pathological examinations improves the diagnostic accuracy of bronchoscopy. Impact Journals LLC 2017-05-24 /pmc/articles/PMC5668052/ /pubmed/29108319 http://dx.doi.org/10.18632/oncotarget.18159 Text en Copyright: © 2017 Goto et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Clinical Research Paper
Goto, Taichiro
Hirotsu, Yosuke
Nakagomi, Takahiro
Shikata, Daichi
Yokoyama, Yujiro
Amemiya, Kenji
Tsutsui, Toshiharu
Kakizaki, Yumiko
Oyama, Toshio
Mochizuki, Hitoshi
Miyashita, Yoshihiro
Omata, Masao
Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
title Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
title_full Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
title_fullStr Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
title_full_unstemmed Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
title_short Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
title_sort detection of tumor-derived dna dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
topic Clinical Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668052/
https://www.ncbi.nlm.nih.gov/pubmed/29108319
http://dx.doi.org/10.18632/oncotarget.18159
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