Cargando…
Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer
The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this imp...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668052/ https://www.ncbi.nlm.nih.gov/pubmed/29108319 http://dx.doi.org/10.18632/oncotarget.18159 |
_version_ | 1783275604929413120 |
---|---|
author | Goto, Taichiro Hirotsu, Yosuke Nakagomi, Takahiro Shikata, Daichi Yokoyama, Yujiro Amemiya, Kenji Tsutsui, Toshiharu Kakizaki, Yumiko Oyama, Toshio Mochizuki, Hitoshi Miyashita, Yoshihiro Omata, Masao |
author_facet | Goto, Taichiro Hirotsu, Yosuke Nakagomi, Takahiro Shikata, Daichi Yokoyama, Yujiro Amemiya, Kenji Tsutsui, Toshiharu Kakizaki, Yumiko Oyama, Toshio Mochizuki, Hitoshi Miyashita, Yoshihiro Omata, Masao |
author_sort | Goto, Taichiro |
collection | PubMed |
description | The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this improves the accuracy of bronchoscopy for diagnosing lung cancer and to identify factors influencing sensitivity. The bronchoscopic sensitivity for diagnosing lung cancer was initially evaluated in 191 patients who underwent lobectomy after bronchoscopy at our hospital. Sputum, bronchial wash fluid, and resected lung cancer specimens were subsequently collected from 18 patients with peripheral small cell lung cancer for genomic analysis. DNA was extracted from formalin-fixed, paraffin-embedded surgical tissue specimens and the supernatant and cell fractions of sputum and bronchial wash fluid. Deep sequencing was performed using a lung cancer panel covering all exons of 53 lung cancer-related genes. The bronchoscopic sensitivity for diagnosing lung cancer at our hospital was 60.7%. Multivariate analysis revealed that this was influenced by tumor size and location, but not histological type or lymph node metastasis. The sensitivity was the highest for biopsy followed by curettage and bronchial wash specimens. DNA mutations homologous to those identified in the primary lesions were detected in the bronchial wash fluid of 10 patients (55.6%), while only 2 patients (11.1%) were diagnosed with lung cancer based on conventional cytological examinations. In conclusion, the addition of genomic analysis to routine pathological examinations improves the diagnostic accuracy of bronchoscopy. |
format | Online Article Text |
id | pubmed-5668052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56680522017-11-04 Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer Goto, Taichiro Hirotsu, Yosuke Nakagomi, Takahiro Shikata, Daichi Yokoyama, Yujiro Amemiya, Kenji Tsutsui, Toshiharu Kakizaki, Yumiko Oyama, Toshio Mochizuki, Hitoshi Miyashita, Yoshihiro Omata, Masao Oncotarget Clinical Research Paper The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this improves the accuracy of bronchoscopy for diagnosing lung cancer and to identify factors influencing sensitivity. The bronchoscopic sensitivity for diagnosing lung cancer was initially evaluated in 191 patients who underwent lobectomy after bronchoscopy at our hospital. Sputum, bronchial wash fluid, and resected lung cancer specimens were subsequently collected from 18 patients with peripheral small cell lung cancer for genomic analysis. DNA was extracted from formalin-fixed, paraffin-embedded surgical tissue specimens and the supernatant and cell fractions of sputum and bronchial wash fluid. Deep sequencing was performed using a lung cancer panel covering all exons of 53 lung cancer-related genes. The bronchoscopic sensitivity for diagnosing lung cancer at our hospital was 60.7%. Multivariate analysis revealed that this was influenced by tumor size and location, but not histological type or lymph node metastasis. The sensitivity was the highest for biopsy followed by curettage and bronchial wash specimens. DNA mutations homologous to those identified in the primary lesions were detected in the bronchial wash fluid of 10 patients (55.6%), while only 2 patients (11.1%) were diagnosed with lung cancer based on conventional cytological examinations. In conclusion, the addition of genomic analysis to routine pathological examinations improves the diagnostic accuracy of bronchoscopy. Impact Journals LLC 2017-05-24 /pmc/articles/PMC5668052/ /pubmed/29108319 http://dx.doi.org/10.18632/oncotarget.18159 Text en Copyright: © 2017 Goto et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Clinical Research Paper Goto, Taichiro Hirotsu, Yosuke Nakagomi, Takahiro Shikata, Daichi Yokoyama, Yujiro Amemiya, Kenji Tsutsui, Toshiharu Kakizaki, Yumiko Oyama, Toshio Mochizuki, Hitoshi Miyashita, Yoshihiro Omata, Masao Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
title | Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
title_full | Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
title_fullStr | Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
title_full_unstemmed | Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
title_short | Detection of tumor-derived DNA dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
title_sort | detection of tumor-derived dna dispersed in the airway improves the diagnostic accuracy of bronchoscopy for lung cancer |
topic | Clinical Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668052/ https://www.ncbi.nlm.nih.gov/pubmed/29108319 http://dx.doi.org/10.18632/oncotarget.18159 |
work_keys_str_mv | AT gototaichiro detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT hirotsuyosuke detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT nakagomitakahiro detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT shikatadaichi detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT yokoyamayujiro detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT amemiyakenji detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT tsutsuitoshiharu detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT kakizakiyumiko detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT oyamatoshio detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT mochizukihitoshi detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT miyashitayoshihiro detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer AT omatamasao detectionoftumorderiveddnadispersedintheairwayimprovesthediagnosticaccuracyofbronchoscopyforlungcancer |