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Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP

Poisonous Entoloma rhodopolium and other similar species including edible E. sarcopum are morphologically diverse. People mistake poisonous species for edible species. Classification and the detection method of these species need to be defined. The morphological and phylogenetic studies have been re...

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Autores principales: Kondo, Kazunari, Nakamura, Kosuke, Ishigaki, Takumi, Sakata, Kozue, Obitsu, Saemi, Noguchi, Akio, Fukuda, Nozomi, Nagasawa, Eiji, Teshima, Reiko, Nishimaki-Mogami, Tomoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668239/
https://www.ncbi.nlm.nih.gov/pubmed/29097736
http://dx.doi.org/10.1038/s41598-017-14466-x
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author Kondo, Kazunari
Nakamura, Kosuke
Ishigaki, Takumi
Sakata, Kozue
Obitsu, Saemi
Noguchi, Akio
Fukuda, Nozomi
Nagasawa, Eiji
Teshima, Reiko
Nishimaki-Mogami, Tomoko
author_facet Kondo, Kazunari
Nakamura, Kosuke
Ishigaki, Takumi
Sakata, Kozue
Obitsu, Saemi
Noguchi, Akio
Fukuda, Nozomi
Nagasawa, Eiji
Teshima, Reiko
Nishimaki-Mogami, Tomoko
author_sort Kondo, Kazunari
collection PubMed
description Poisonous Entoloma rhodopolium and other similar species including edible E. sarcopum are morphologically diverse. People mistake poisonous species for edible species. Classification and the detection method of these species need to be defined. The morphological and phylogenetic studies have been reported in northern Europe. In Japan, the genetic study remains unsolved. Thus, phylogenetic analysis of E. rhodopolium was conducted using ITS and RPB2 sequences, and the result was compared with that of European species. Japanese E. rhodopolium was classified into three clades, none of which belonged to the true European E. rhodopolium and other known species. Three species were defined as new species. Entoloma rhodopolium clade-I (named E. lacus) was genetically close to but morphologically separated from E. majaloides. Clade-II (E. subrhodopolium) was classified to the same group as E. sinuatum and E. subsinuatum, but distinct from these species. Clade-III was segregated from known Entoloma species including E. lupinum, and named E. pseudorhodopolium. Based on the classification, a simple identification method PCR-RFLP was developed to discriminate between poisonous species and edible E. sarcopum, which is very similar in morphology. The study can help to clarify the taxonomy of complex E. rhodopolium-related species, and to prevent food poisoning.
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spelling pubmed-56682392017-11-08 Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP Kondo, Kazunari Nakamura, Kosuke Ishigaki, Takumi Sakata, Kozue Obitsu, Saemi Noguchi, Akio Fukuda, Nozomi Nagasawa, Eiji Teshima, Reiko Nishimaki-Mogami, Tomoko Sci Rep Article Poisonous Entoloma rhodopolium and other similar species including edible E. sarcopum are morphologically diverse. People mistake poisonous species for edible species. Classification and the detection method of these species need to be defined. The morphological and phylogenetic studies have been reported in northern Europe. In Japan, the genetic study remains unsolved. Thus, phylogenetic analysis of E. rhodopolium was conducted using ITS and RPB2 sequences, and the result was compared with that of European species. Japanese E. rhodopolium was classified into three clades, none of which belonged to the true European E. rhodopolium and other known species. Three species were defined as new species. Entoloma rhodopolium clade-I (named E. lacus) was genetically close to but morphologically separated from E. majaloides. Clade-II (E. subrhodopolium) was classified to the same group as E. sinuatum and E. subsinuatum, but distinct from these species. Clade-III was segregated from known Entoloma species including E. lupinum, and named E. pseudorhodopolium. Based on the classification, a simple identification method PCR-RFLP was developed to discriminate between poisonous species and edible E. sarcopum, which is very similar in morphology. The study can help to clarify the taxonomy of complex E. rhodopolium-related species, and to prevent food poisoning. Nature Publishing Group UK 2017-11-02 /pmc/articles/PMC5668239/ /pubmed/29097736 http://dx.doi.org/10.1038/s41598-017-14466-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kondo, Kazunari
Nakamura, Kosuke
Ishigaki, Takumi
Sakata, Kozue
Obitsu, Saemi
Noguchi, Akio
Fukuda, Nozomi
Nagasawa, Eiji
Teshima, Reiko
Nishimaki-Mogami, Tomoko
Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP
title Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP
title_full Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP
title_fullStr Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP
title_full_unstemmed Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP
title_short Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP
title_sort molecular phylogenetic analysis of new entoloma rhodopolium-related species in japan and its identification method using pcr-rflp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668239/
https://www.ncbi.nlm.nih.gov/pubmed/29097736
http://dx.doi.org/10.1038/s41598-017-14466-x
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