Cargando…

Structural and functional comparison of hexahistidine tagged and untagged forms of small multidrug resistance protein, EmrE

EmrE is a member of the small multidrug resistance (SMR) protein family in Escherichia coli. EmrE confers resistance to a wide variety of quaternary cation compounds (QCCs) as an efflux transporter driven by proton motive force. The purification yield of most membrane proteins are challenging becaus...

Descripción completa

Detalles Bibliográficos
Autores principales:	Qazi, S. Junaid S., Chew, Raymond, Bay, Denice C., Turner, Raymond J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668558/ https://www.ncbi.nlm.nih.gov/pubmed/29124131 http://dx.doi.org/10.1016/j.bbrep.2015.03.007

Ejemplares similares

Influence of quaternary cation compound on the size of the Escherichia coli small multidrug resistance protein, EmrE
por: Qazi, S. Junaid S., et al.
Publicado: (2018)

Diversity and evolution of the small multidrug resistance protein family
por: Bay, Denice C, et al.
Publicado: (2009)

Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis
por: Schoonen, Lise, et al.
Publicado: (2017)

An active site–tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase
por: Murphy, Jesse R., et al.
Publicado: (2015)

Jet production in untagged $\gamma\gamma$ collisions and single tagged inclusive hadron production at LEP with DELPHI
por: Kapusta, F
Publicado: (1992)

Electrostatic lock in the transport cycle of the multidrug resistance transporter EmrE
por: Vermaas, Josh V., et al.
Publicado: (2018)

Electron Crystallography Reveals Plasticity within the Drug Binding Site of the Small Multidrug Transporter EmrE
por: Korkhov, Vladimir M., et al.
Publicado: (2008)

Crystal structures of bacterial small multidrug resistance transporter EmrE in complex with structurally diverse substrates
por: Kermani, Ali A, et al.
Publicado: (2022)

ALICE measurements of inclusive untagged and heavy flavor-tagged jets in pp, p-Pb and Pb-Pb collisions
por: Isakov, Artem
Publicado: (2022)

Production of e, $\mu$ and $\tau$ Pairs in Untagged Two-Photon Collisions at LEP
por: Acciarri, M, et al.
Publicado: (1997)

Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA
por: Juma, Kevin Maafu, et al.
Publicado: (2022)

Intrinsic Conformational Plasticity of Native EmrE Provides a Pathway for Multidrug Resistance
por: Cho, Min-Kyu, et al.
Publicado: (2014)

Efficient Delivery of Antibodies Intracellularly by Co-Assembly with Hexahistidine-Metal Assemblies (HmA)
por: Wei, Shaoyin, et al.
Publicado: (2021)

Co-delivery of nigericin and decitabine using hexahistidine-metal nanocarriers for pyroptosis-induced immunotherapeutics
por: Niu, Qiang, et al.
Publicado: (2022)

Asymmetric protonation of EmrE
por: Morrison, Emma A., et al.
Publicado: (2015)

Charge neutralization of the active site glutamates does not limit substrate binding and transport by small multidrug resistance transporter EmrE
por: Spreacker, Peyton J., et al.
Publicado: (2022)

Expression and purification of untagged GlnK proteins from actinobacteria
por: Gerhardt, Edileusa C.M., et al.
Publicado: (2017)

NarJ subfamily system specific chaperone diversity and evolution is directed by respiratory enzyme associations
por: Bay, Denice C., et al.
Publicado: (2015)

An emerging consensus for the structure of EmrE
por: Korkhov, Vladimir M., et al.
Publicado: (2009)

Measurement of $\eta_{c}$ production in untagged two-photon collisions at LEP
por: Adriani, O, et al.
Publicado: (1993)

Untagged Analysis of B0s -> J/psi phi Channel
por: Charles, François
Publicado: (1999)

Fungal plant pathogen “mutagenomics” reveals tagged and untagged mutations in Zymoseptoria tritici and identifies SSK2 as key morphogenesis and stress-responsive virulence factor
por: Blyth, Hannah R., et al.
Publicado: (2023)

Development of a hexahistidine-3× FLAG-tandem affinity purification method for endogenous protein complexes in Pichia pastoris
por: Higo, Toshiaki, et al.
Publicado: (2014)

Streamlined, automated protocols for the production of milligram quantities of untagged recombinant human cyclophilin-A (hCypA) and untagged human proliferating cell nuclear antigen (hPCNA) using ÄKTAxpress™
por: Ludwig, Cornelia, et al.
Publicado: (2010)

Diversity and Evolution of Bacterial Twin Arginine Translocase Protein, TatC, Reveals a Protein Secretion System That Is Evolving to Fit Its Environmental Niche
por: Simone, Domenico, et al.
Publicado: (2013)

Untagged $B \to X_{(s+d)}$ gamma CP asymmetry as a probe for new physics
por: Hurth, Tobias, et al.
Publicado: (2003)

Measuring the CKM angle $\gamma$ at LHCb using untagged $B_s \to D \phi$ decays
por: Ricciardi, S
Publicado: (2010)

Perplexing new insight into the dynamics of the EmrE transporter
por: Sanders, Charles R.
Publicado: (2015)

Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein
por: Manuel-Cabrera, Carlos Alberto, et al.
Publicado: (2016)

A Genome-Wide Assessment of the Role of Untagged Copy Number Variants in Type 1 Diabetes
por: Zanda, Manuela, et al.
Publicado: (2014)

Substrate-Free Untagged Detection of miR393a Using an Ultrasensitive Electrochemical Biosensor
por: Nehra, Anuj, et al.
Publicado: (2022)

Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting
por: Boselli, Daniela, et al.
Publicado: (2023)

The level-1 charged particle trigger of L3 detector and the study of two-photon production of $e^{+}e^{-}$ and $\mu^{+}\mu^{-}$ pairs in untagged events
por: Hoorani, H
Publicado: (1996)

EMR in Eye Units
por: Camburu, Georgiana, et al.
Publicado: (2020)

EmrE reminds us to expect the unexpected in membrane transport
por: Grabe, Michael, et al.
Publicado: (2019)

The ‘Anchoring-EMR’ technique has already been described and named the ‘Tip-in EMR’ technique
por: Imai, Kenichiro, et al.
Publicado: (2020)

Combining structured and unstructured data in EMRs to create clinically-defined EMR-derived cohorts
por: Tam, Charmaine S., et al.
Publicado: (2021)

High-throughput Isolation and Characterization of Untagged Membrane Protein Complexes: Outer Membrane Complexes of Desulfovibrio vulgaris
por: Walian, Peter J., et al.
Publicado: (2012)

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
por: Reif, Andreas, et al.
Publicado: (2018)

Correction: Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting
por: Boselli, Daniela, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_curkjn3fte2et1jl3atoud8rbn