Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties

Alkaline protease from brinjal leaf (Solanum melongena) having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg. SDS-PAGE of the final preparation revealed a single protein band of approx 14 kDa. Purified enzyme was characterized and was successfully i...

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Autores principales: Kumari, Arpana, Kaur, Balwinder, Srivastava, Rajendra, Sangwan, Rajender S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668643/
https://www.ncbi.nlm.nih.gov/pubmed/29124151
http://dx.doi.org/10.1016/j.bbrep.2015.05.009
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author Kumari, Arpana
Kaur, Balwinder
Srivastava, Rajendra
Sangwan, Rajender S.
author_facet Kumari, Arpana
Kaur, Balwinder
Srivastava, Rajendra
Sangwan, Rajender S.
author_sort Kumari, Arpana
collection PubMed
description Alkaline protease from brinjal leaf (Solanum melongena) having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg. SDS-PAGE of the final preparation revealed a single protein band of approx 14 kDa. Purified enzyme was characterized and was successfully immobilized into the amorphous mesoporous silica (SBA-15) and crystalline mesoporous zeolite (Nano-ZSM-5) using entrapment method. Maximum immobilization of 63.5% and 79.77% was obtained with SBA-15 and Nano-ZSM-5, respectively. This protocol serves as a novel approach for bioprocesses, mainly as milk coagulant for local dairy products and particularly, cheese making, and opens the new dimension of further research and other innovation.
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spelling pubmed-56686432017-11-09 Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties Kumari, Arpana Kaur, Balwinder Srivastava, Rajendra Sangwan, Rajender S. Biochem Biophys Rep Research Article Alkaline protease from brinjal leaf (Solanum melongena) having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 U/mg. SDS-PAGE of the final preparation revealed a single protein band of approx 14 kDa. Purified enzyme was characterized and was successfully immobilized into the amorphous mesoporous silica (SBA-15) and crystalline mesoporous zeolite (Nano-ZSM-5) using entrapment method. Maximum immobilization of 63.5% and 79.77% was obtained with SBA-15 and Nano-ZSM-5, respectively. This protocol serves as a novel approach for bioprocesses, mainly as milk coagulant for local dairy products and particularly, cheese making, and opens the new dimension of further research and other innovation. Elsevier 2015-05-29 /pmc/articles/PMC5668643/ /pubmed/29124151 http://dx.doi.org/10.1016/j.bbrep.2015.05.009 Text en © 2015 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Kumari, Arpana
Kaur, Balwinder
Srivastava, Rajendra
Sangwan, Rajender S.
Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties
title Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties
title_full Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties
title_fullStr Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties
title_full_unstemmed Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties
title_short Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties
title_sort isolation and immobilization of alkaline protease on mesoporous silica and mesoporous zsm-5 zeolite materials for improved catalytic properties
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668643/
https://www.ncbi.nlm.nih.gov/pubmed/29124151
http://dx.doi.org/10.1016/j.bbrep.2015.05.009
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