Cargando…

The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer

SCO (Synthesis of Cytochrome c Oxidase) proteins are present in prokaryotic and eukaryotic cells, and are often required for efficient synthesis of the respiratory enzyme cytochrome c oxidase. The Bacillus subtilis version of SCO (i.e., BsSCO) has much greater affinity for Cu(II) than it does for Cu...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Shuai, Andrews, Diann, Hill, Bruce C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668878/
https://www.ncbi.nlm.nih.gov/pubmed/29124182
http://dx.doi.org/10.1016/j.bbrep.2015.08.010
_version_ 1783275751833862144
author Xu, Shuai
Andrews, Diann
Hill, Bruce C.
author_facet Xu, Shuai
Andrews, Diann
Hill, Bruce C.
author_sort Xu, Shuai
collection PubMed
description SCO (Synthesis of Cytochrome c Oxidase) proteins are present in prokaryotic and eukaryotic cells, and are often required for efficient synthesis of the respiratory enzyme cytochrome c oxidase. The Bacillus subtilis version of SCO (i.e., BsSCO) has much greater affinity for Cu(II) than it does for Cu(I) (Davidson and Hill, 2009), and this has been contrasted to mitochondrial SCO proteins that are characterized as being specific for Cu(I) (Nittis, George and Winge, 2001). This differential affinity has been proposed to reflect the different physiological environments in which these two members of the SCO protein family reside. In this study the affinity of mitochondrial SCO1 from yeast is compared directly to that of BsSCO in vitro. We find that the yeast SCO1 protein has similar preference for Cu(II) over Cu(I), as does BsSCO. We propose a mechanism for SCO function which would involve high-affinity binding to capture Cu(II), and relatively weak binding of Cu(I) to facilitate copper transfer.
format Online
Article
Text
id pubmed-5668878
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-56688782017-11-09 The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer Xu, Shuai Andrews, Diann Hill, Bruce C. Biochem Biophys Rep Research Article SCO (Synthesis of Cytochrome c Oxidase) proteins are present in prokaryotic and eukaryotic cells, and are often required for efficient synthesis of the respiratory enzyme cytochrome c oxidase. The Bacillus subtilis version of SCO (i.e., BsSCO) has much greater affinity for Cu(II) than it does for Cu(I) (Davidson and Hill, 2009), and this has been contrasted to mitochondrial SCO proteins that are characterized as being specific for Cu(I) (Nittis, George and Winge, 2001). This differential affinity has been proposed to reflect the different physiological environments in which these two members of the SCO protein family reside. In this study the affinity of mitochondrial SCO1 from yeast is compared directly to that of BsSCO in vitro. We find that the yeast SCO1 protein has similar preference for Cu(II) over Cu(I), as does BsSCO. We propose a mechanism for SCO function which would involve high-affinity binding to capture Cu(II), and relatively weak binding of Cu(I) to facilitate copper transfer. Elsevier 2015-08-14 /pmc/articles/PMC5668878/ /pubmed/29124182 http://dx.doi.org/10.1016/j.bbrep.2015.08.010 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Xu, Shuai
Andrews, Diann
Hill, Bruce C.
The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer
title The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer
title_full The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer
title_fullStr The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer
title_full_unstemmed The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer
title_short The affinity of yeast and bacterial SCO proteins for CU(I) and CU(II). A capture and release strategy for copper transfer
title_sort affinity of yeast and bacterial sco proteins for cu(i) and cu(ii). a capture and release strategy for copper transfer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668878/
https://www.ncbi.nlm.nih.gov/pubmed/29124182
http://dx.doi.org/10.1016/j.bbrep.2015.08.010
work_keys_str_mv AT xushuai theaffinityofyeastandbacterialscoproteinsforcuiandcuiiacaptureandreleasestrategyforcoppertransfer
AT andrewsdiann theaffinityofyeastandbacterialscoproteinsforcuiandcuiiacaptureandreleasestrategyforcoppertransfer
AT hillbrucec theaffinityofyeastandbacterialscoproteinsforcuiandcuiiacaptureandreleasestrategyforcoppertransfer
AT xushuai affinityofyeastandbacterialscoproteinsforcuiandcuiiacaptureandreleasestrategyforcoppertransfer
AT andrewsdiann affinityofyeastandbacterialscoproteinsforcuiandcuiiacaptureandreleasestrategyforcoppertransfer
AT hillbrucec affinityofyeastandbacterialscoproteinsforcuiandcuiiacaptureandreleasestrategyforcoppertransfer