A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit

The seamless ligation cloning extract (SLiCE) method is a novel seamless DNA cloning tool that utilizes homologous recombination activities in Escherichia coli cell lysates to assemble DNA fragments into a vector. Several laboratory E. coli strains can be used as a source for the SLiCE extract; ther...

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Detalles Bibliográficos
Autores principales: Okegawa, Yuki, Motohashi, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668909/
https://www.ncbi.nlm.nih.gov/pubmed/29124198
http://dx.doi.org/10.1016/j.bbrep.2015.09.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668909/
https://www.ncbi.nlm.nih.gov/pubmed/29124198
http://dx.doi.org/10.1016/j.bbrep.2015.09.005

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