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Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia
BACKGROUND: Plumeria acutifolia (Apocynaceae) is an ornamental plant, used in the traditional medicine and known to have a variety of constituents as alkaloids, flavonoids, and iridoids. Extracts of this plant were proved to have antimicrobial and anticancer activities. OBJECTIVES: This research was...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669089/ https://www.ncbi.nlm.nih.gov/pubmed/29142406 http://dx.doi.org/10.4103/pm.pm_22_17 |
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author | Alhozaimy, Ghadah A. Al-Sheddi, Ebtesam Saad Ibrahim, Taghreed A. |
author_facet | Alhozaimy, Ghadah A. Al-Sheddi, Ebtesam Saad Ibrahim, Taghreed A. |
author_sort | Alhozaimy, Ghadah A. |
collection | PubMed |
description | BACKGROUND: Plumeria acutifolia (Apocynaceae) is an ornamental plant, used in the traditional medicine and known to have a variety of constituents as alkaloids, flavonoids, and iridoids. Extracts of this plant were proved to have antimicrobial and anticancer activities. OBJECTIVES: This research was conducted for the evaluation of the biological activities of P. acutifolia stem bark and isolation and structural elucidation of various chemical compounds from the biologically active fractions. MATERIALS AND METHODS: Methanol extract of stem bark of P. acutifolia was successively fractionated with petroleum ether, dichloromethane, ethyl acetate, and n-butanol. The fractions were evaluated for their antimicrobial, cytotoxic, and antioxidant activities. Fractions with promising biological activities were subjected to chromatographic techniques for the isolation of compounds, followed by structural elucidation using several spectroscopic techniques. RESULTS: P. acutifolia stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against Syncephalastrum racemosum (7.81 μg/ml) and Escherichia coli (3.9 μg/ml). The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction, using concentrations of 1, 2.5, 5, and 10 μl/ml. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect, with an IC(50) of 197.1 μg/ml. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5). CONCLUSION: P. acutifolia was proved to have significant antimicrobial, cytotoxic, and antioxidant activities; the isolated compounds were flavonoids, iridoids, and secoiridoid, some of which were reported for the first time in genus Plumeria and/or family Apocynaceae. SUMMARY: P. acutifolia stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against Syncephalastrum racemosum and Escherichia coli. The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5). [Image: see text] Abbreviations used: mp: Melting point, NMR: Nuclear magnetic resonance, s: Singlet, d: Double, t: Triplet, q: Quartet, dd: Double-double, m: Multiplet, br: Broad. |
format | Online Article Text |
id | pubmed-5669089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56690892017-11-15 Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia Alhozaimy, Ghadah A. Al-Sheddi, Ebtesam Saad Ibrahim, Taghreed A. Pharmacogn Mag Original Article BACKGROUND: Plumeria acutifolia (Apocynaceae) is an ornamental plant, used in the traditional medicine and known to have a variety of constituents as alkaloids, flavonoids, and iridoids. Extracts of this plant were proved to have antimicrobial and anticancer activities. OBJECTIVES: This research was conducted for the evaluation of the biological activities of P. acutifolia stem bark and isolation and structural elucidation of various chemical compounds from the biologically active fractions. MATERIALS AND METHODS: Methanol extract of stem bark of P. acutifolia was successively fractionated with petroleum ether, dichloromethane, ethyl acetate, and n-butanol. The fractions were evaluated for their antimicrobial, cytotoxic, and antioxidant activities. Fractions with promising biological activities were subjected to chromatographic techniques for the isolation of compounds, followed by structural elucidation using several spectroscopic techniques. RESULTS: P. acutifolia stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against Syncephalastrum racemosum (7.81 μg/ml) and Escherichia coli (3.9 μg/ml). The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction, using concentrations of 1, 2.5, 5, and 10 μl/ml. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect, with an IC(50) of 197.1 μg/ml. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5). CONCLUSION: P. acutifolia was proved to have significant antimicrobial, cytotoxic, and antioxidant activities; the isolated compounds were flavonoids, iridoids, and secoiridoid, some of which were reported for the first time in genus Plumeria and/or family Apocynaceae. SUMMARY: P. acutifolia stem bark showed a significant antimicrobial activity, where the ethyl acetate fraction was active against Syncephalastrum racemosum and Escherichia coli. The cytotoxic activity against HEPG-2, HCT-116, and MCF-7 cell lines was highest in the petroleum ether fraction. The antioxidant activity was concentration dependent; ethyl acetate fraction showed the most predominant effect. Five compounds were identified as narcissin (1), quercitrin (2), sweroside (3), gaertneroside (4), and plumieride (5). [Image: see text] Abbreviations used: mp: Melting point, NMR: Nuclear magnetic resonance, s: Singlet, d: Double, t: Triplet, q: Quartet, dd: Double-double, m: Multiplet, br: Broad. Medknow Publications & Media Pvt Ltd 2017-10 2017-10-11 /pmc/articles/PMC5669089/ /pubmed/29142406 http://dx.doi.org/10.4103/pm.pm_22_17 Text en Copyright: © 2017 Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Alhozaimy, Ghadah A. Al-Sheddi, Ebtesam Saad Ibrahim, Taghreed A. Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia |
title | Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia |
title_full | Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia |
title_fullStr | Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia |
title_full_unstemmed | Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia |
title_short | Biological Activity and Isolation of Compounds from Stem Bark of Plumeria acutifolia |
title_sort | biological activity and isolation of compounds from stem bark of plumeria acutifolia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669089/ https://www.ncbi.nlm.nih.gov/pubmed/29142406 http://dx.doi.org/10.4103/pm.pm_22_17 |
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