Screening of Norharmane from Seven Cyanobacteria by High-performance Liquid Chromatography

BACKGROUND: Cyanobacteria, including pharmaceutically and medicinally valuable compounds attract the great attention lately. Norharmane (9H-pyrido (3,4-b) indole found in some cyanobacteria revealed a great number of biological effects. OBJECTIVE: Seven cyanobacteria were isolated and identified from Yesilirmak River and Gaziosmanpasa University Campus to determine the norharmane content. MATERIALS AND METHODS: Cyanobacteria collected from Tokat, Turkey were isolated and identified by morphologically. Norharmane (9H-pyrido [3,4-b] indole) quantities were presented for seven cyanobacteria, Chroococcus minutus (Kütz.) Nägeli, Geitlerinema carotinosum (Geitler) Anagnostidis, Nostoc linckia Bornet ex Bornet and Flahault, Anabaena oryzae F. E. Fritsch, Oscillatoria limnetica Lemmermann, Phormidium sp. Kützing ex Gomont, and Cylindrospermum sp. Kutzing ex E. Bornet and C. Flahault by high-performance liquid chromatography. RESULTS: The norharmane amount indicated for cyanobacterial culture media altered in a species-dependent kind in the range of 0.81–10.87 μg/g. C. minutus produced the most norharmane among the investigated cyanobacteria as 10.87 μg/g. CONCLUSION: Cyanobacteria could be an important source of norharmane as well as pharmaceutically valuable compounds. SUMMARY: Seven cyanobacteria were isolated and identified from Yesilirmak River. Quantitative analysis of norharmane was executed on isolated cyanobacteria. Four cyanobecteria species included the norharmane. Chroococcus minutus contained the most norharmane (10.87 μg/g). [Image: see text] Abbreviations used: HPLC: High performance liquid chromatograph.