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Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng

Sclerotinia ginseng is a necrotrophic soil pathogen that mainly infects the root and basal stem of ginseng, causing serious commercial losses. Sclerotia, which are important in the fungal life cycle, are hard, asexual, resting structures that can survive in soil for several years. Generally, sclerot...

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Autores principales: Wang, Dan, Fu, Jun Fan, Zhou, Ru Jun, Li, Zi Bo, Xie, Yu Jiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669253/
https://www.ncbi.nlm.nih.gov/pubmed/29104825
http://dx.doi.org/10.7717/peerj.3979
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author Wang, Dan
Fu, Jun Fan
Zhou, Ru Jun
Li, Zi Bo
Xie, Yu Jiao
author_facet Wang, Dan
Fu, Jun Fan
Zhou, Ru Jun
Li, Zi Bo
Xie, Yu Jiao
author_sort Wang, Dan
collection PubMed
description Sclerotinia ginseng is a necrotrophic soil pathogen that mainly infects the root and basal stem of ginseng, causing serious commercial losses. Sclerotia, which are important in the fungal life cycle, are hard, asexual, resting structures that can survive in soil for several years. Generally, sclerotium development is accompanied by the exudation of droplets. Here, the yellowish droplets of S. ginseng were first examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the proteome was identified by a combination of different analytical platforms. A total of 59 proteins were identified and classified into six categories: carbohydrate metabolism (39%), oxidation-reduction process (12%), transport and catabolism (5%), amino acid metabolism (3%), other functions (18%), and unknown protein (23%), which exhibited considerable differences in protein composition compared with droplets of S. sclerotium. In the carbohydrate metabolism group, several proteins were associated with sclerotium development, particularly fungal cell wall formation. The pathogenicity and virulence of the identified proteins are also discussed in this report. The findings of this study may improve our understanding of the function of exudate droplets as well as the life cycle and pathogenesis of S. ginseng.
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spelling pubmed-56692532017-11-03 Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng Wang, Dan Fu, Jun Fan Zhou, Ru Jun Li, Zi Bo Xie, Yu Jiao PeerJ Agricultural Science Sclerotinia ginseng is a necrotrophic soil pathogen that mainly infects the root and basal stem of ginseng, causing serious commercial losses. Sclerotia, which are important in the fungal life cycle, are hard, asexual, resting structures that can survive in soil for several years. Generally, sclerotium development is accompanied by the exudation of droplets. Here, the yellowish droplets of S. ginseng were first examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the proteome was identified by a combination of different analytical platforms. A total of 59 proteins were identified and classified into six categories: carbohydrate metabolism (39%), oxidation-reduction process (12%), transport and catabolism (5%), amino acid metabolism (3%), other functions (18%), and unknown protein (23%), which exhibited considerable differences in protein composition compared with droplets of S. sclerotium. In the carbohydrate metabolism group, several proteins were associated with sclerotium development, particularly fungal cell wall formation. The pathogenicity and virulence of the identified proteins are also discussed in this report. The findings of this study may improve our understanding of the function of exudate droplets as well as the life cycle and pathogenesis of S. ginseng. PeerJ Inc. 2017-10-31 /pmc/articles/PMC5669253/ /pubmed/29104825 http://dx.doi.org/10.7717/peerj.3979 Text en ©2017 Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Wang, Dan
Fu, Jun Fan
Zhou, Ru Jun
Li, Zi Bo
Xie, Yu Jiao
Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng
title Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng
title_full Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng
title_fullStr Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng
title_full_unstemmed Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng
title_short Proteomics research and related functional classification of liquid sclerotial exudates of Sclerotinia ginseng
title_sort proteomics research and related functional classification of liquid sclerotial exudates of sclerotinia ginseng
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669253/
https://www.ncbi.nlm.nih.gov/pubmed/29104825
http://dx.doi.org/10.7717/peerj.3979
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