Cargando…
Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups
Several plant and animal species of agricultural importance are commercialized as hybrids to take advantage of the heterosis phenomenon. Understanding the genetic architecture of hybrid performances is therefore of key importance. We developed two multiparental maize (Zea mays L.) populations, each...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669627/ https://www.ncbi.nlm.nih.gov/pubmed/28971957 http://dx.doi.org/10.1534/genetics.117.300305 |
_version_ | 1783275873521106944 |
---|---|
author | Giraud, Héloïse Bauland, Cyril Falque, Matthieu Madur, Delphine Combes, Valérie Jamin, Philippe Monteil, Cécile Laborde, Jacques Palaffre, Carine Gaillard, Antoine Blanchard, Philippe Charcosset, Alain Moreau, Laurence |
author_facet | Giraud, Héloïse Bauland, Cyril Falque, Matthieu Madur, Delphine Combes, Valérie Jamin, Philippe Monteil, Cécile Laborde, Jacques Palaffre, Carine Gaillard, Antoine Blanchard, Philippe Charcosset, Alain Moreau, Laurence |
author_sort | Giraud, Héloïse |
collection | PubMed |
description | Several plant and animal species of agricultural importance are commercialized as hybrids to take advantage of the heterosis phenomenon. Understanding the genetic architecture of hybrid performances is therefore of key importance. We developed two multiparental maize (Zea mays L.) populations, each corresponding to an important heterotic group (dent or flint) and comprised of six connected biparental segregating populations of inbred lines (802 and 822 lines for each group, respectively) issued from four founder lines. Instead of using “testers” to evaluate their hybrid values, segregating lines were crossed according to an incomplete factorial design to produce 951 dent–flint hybrids, evaluated for four biomass production traits in eight environments. QTL detection was carried out for the general-combining-ability (GCA) and specific-combining-ability (SCA) components of hybrid value, considering allelic effects transmitted from each founder line. In total, 42 QTL were detected across traits. We detected mostly QTL affecting GCA, 31% (41% for dry matter yield) of which also had mild effects on SCA. The small impact of dominant effects is consistent with the known differentiation between the dent and flint heterotic groups and the small percentage of hybrid variance due to SCA observed in our design (∼20% for the different traits). Furthermore, most (80%) of GCA QTL were segregating in only one of the two heterotic groups. Relative to tester-based designs, use of hybrids between two multiparental populations appears highly cost efficient to detect QTL in two heterotic groups simultaneously. This presents new prospects for selecting superior hybrid combinations with markers. |
format | Online Article Text |
id | pubmed-5669627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-56696272017-11-09 Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups Giraud, Héloïse Bauland, Cyril Falque, Matthieu Madur, Delphine Combes, Valérie Jamin, Philippe Monteil, Cécile Laborde, Jacques Palaffre, Carine Gaillard, Antoine Blanchard, Philippe Charcosset, Alain Moreau, Laurence Genetics Investigations Several plant and animal species of agricultural importance are commercialized as hybrids to take advantage of the heterosis phenomenon. Understanding the genetic architecture of hybrid performances is therefore of key importance. We developed two multiparental maize (Zea mays L.) populations, each corresponding to an important heterotic group (dent or flint) and comprised of six connected biparental segregating populations of inbred lines (802 and 822 lines for each group, respectively) issued from four founder lines. Instead of using “testers” to evaluate their hybrid values, segregating lines were crossed according to an incomplete factorial design to produce 951 dent–flint hybrids, evaluated for four biomass production traits in eight environments. QTL detection was carried out for the general-combining-ability (GCA) and specific-combining-ability (SCA) components of hybrid value, considering allelic effects transmitted from each founder line. In total, 42 QTL were detected across traits. We detected mostly QTL affecting GCA, 31% (41% for dry matter yield) of which also had mild effects on SCA. The small impact of dominant effects is consistent with the known differentiation between the dent and flint heterotic groups and the small percentage of hybrid variance due to SCA observed in our design (∼20% for the different traits). Furthermore, most (80%) of GCA QTL were segregating in only one of the two heterotic groups. Relative to tester-based designs, use of hybrids between two multiparental populations appears highly cost efficient to detect QTL in two heterotic groups simultaneously. This presents new prospects for selecting superior hybrid combinations with markers. Genetics Society of America 2017-11 2017-10-25 /pmc/articles/PMC5669627/ /pubmed/28971957 http://dx.doi.org/10.1534/genetics.117.300305 Text en Copyright © 2017 by the Genetics Society of America Available freely online through the author-supported open access option. |
spellingShingle | Investigations Giraud, Héloïse Bauland, Cyril Falque, Matthieu Madur, Delphine Combes, Valérie Jamin, Philippe Monteil, Cécile Laborde, Jacques Palaffre, Carine Gaillard, Antoine Blanchard, Philippe Charcosset, Alain Moreau, Laurence Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups |
title | Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups |
title_full | Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups |
title_fullStr | Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups |
title_full_unstemmed | Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups |
title_short | Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups |
title_sort | reciprocal genetics: identifying qtl for general and specific combining abilities in hybrids between multiparental populations from two maize (zea mays l.) heterotic groups |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669627/ https://www.ncbi.nlm.nih.gov/pubmed/28971957 http://dx.doi.org/10.1534/genetics.117.300305 |
work_keys_str_mv | AT giraudheloise reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT baulandcyril reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT falquematthieu reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT madurdelphine reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT combesvalerie reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT jaminphilippe reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT monteilcecile reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT labordejacques reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT palaffrecarine reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT gaillardantoine reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT blanchardphilippe reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT charcossetalain reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups AT moreaulaurence reciprocalgeneticsidentifyingqtlforgeneralandspecificcombiningabilitiesinhybridsbetweenmultiparentalpopulationsfromtwomaizezeamayslheteroticgroups |