Cargando…
Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669851/ https://www.ncbi.nlm.nih.gov/pubmed/29137225 http://dx.doi.org/10.18632/oncotarget.18998 |
_version_ | 1783275913490726912 |
---|---|
author | Zou, Meisheng Huang, Chixiong Li, Xinzhong He, Xiang Chen, Yanmei Liao, Wangjun Liao, Yulin Sun, Jie Liu, Ze Zhong, Lintao Bin, Jianping |
author_facet | Zou, Meisheng Huang, Chixiong Li, Xinzhong He, Xiang Chen, Yanmei Liao, Wangjun Liao, Yulin Sun, Jie Liu, Ze Zhong, Lintao Bin, Jianping |
author_sort | Zou, Meisheng |
collection | PubMed |
description | OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA; n=3) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was used to validate the expression pattern of circRNAs, parental genes, and hsa-miR-320a; Western blotting confirmed MMP9 expression with additional samples. Bioinformatic tools including network analysis, Gene ontology, and KEGG pathway analysis were utilized. RESULTS: Among 8,173 detected circRNA genes, 156 upregulated and 106 downregulated significantly in human TAD as compared to NA (P£0.05). Quantitative real-time PCR showed an elevated expression of the upregulated hsa_circRNA_101238, hsa_circRNA_104634, hsa_circRNA_002271, hsa_circRNA_102771, hsa_circRNA_104349, COL1A1, and COL6A3 and reduced of the downregulated hsa_circRNA_102683, hsa_circRNA_005525, hsa_circRNA_103458, and FLNA. Gene ontology analysis revealed that the parental genes favored several pathological processes, such as negative regulation of cell proliferation and extracellular matrix organization. The circRNA-miRNA co-expression network predicted that 33 circRNAs might interact with at least one target miRNAs altered in TAD. KEGG pathway analysis revealed that 28 altered miRNAs were enriched on focal adhesion and vascular smooth muscle contraction. The hsa_circRNA_101238-miRNA-mRNA network indicated the highest degree of hsa-miR-320a. Quantitative real-time PCR and Western blot manifested the low expression of hsa-miR-320a and high of MMP9 in human TAD tissues, respectively. CONCLUSIONS: This study revealed hundreds of differentially expressed circular RNAs in human TAD, suggesting that hsa_circRNA_101238 might inhibit the expression of hsa-miR-320a and increase that of MMP9 in TAD. |
format | Online Article Text |
id | pubmed-5669851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56698512017-11-09 Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection Zou, Meisheng Huang, Chixiong Li, Xinzhong He, Xiang Chen, Yanmei Liao, Wangjun Liao, Yulin Sun, Jie Liu, Ze Zhong, Lintao Bin, Jianping Oncotarget Research Paper: Pathology OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA; n=3) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was used to validate the expression pattern of circRNAs, parental genes, and hsa-miR-320a; Western blotting confirmed MMP9 expression with additional samples. Bioinformatic tools including network analysis, Gene ontology, and KEGG pathway analysis were utilized. RESULTS: Among 8,173 detected circRNA genes, 156 upregulated and 106 downregulated significantly in human TAD as compared to NA (P£0.05). Quantitative real-time PCR showed an elevated expression of the upregulated hsa_circRNA_101238, hsa_circRNA_104634, hsa_circRNA_002271, hsa_circRNA_102771, hsa_circRNA_104349, COL1A1, and COL6A3 and reduced of the downregulated hsa_circRNA_102683, hsa_circRNA_005525, hsa_circRNA_103458, and FLNA. Gene ontology analysis revealed that the parental genes favored several pathological processes, such as negative regulation of cell proliferation and extracellular matrix organization. The circRNA-miRNA co-expression network predicted that 33 circRNAs might interact with at least one target miRNAs altered in TAD. KEGG pathway analysis revealed that 28 altered miRNAs were enriched on focal adhesion and vascular smooth muscle contraction. The hsa_circRNA_101238-miRNA-mRNA network indicated the highest degree of hsa-miR-320a. Quantitative real-time PCR and Western blot manifested the low expression of hsa-miR-320a and high of MMP9 in human TAD tissues, respectively. CONCLUSIONS: This study revealed hundreds of differentially expressed circular RNAs in human TAD, suggesting that hsa_circRNA_101238 might inhibit the expression of hsa-miR-320a and increase that of MMP9 in TAD. Impact Journals LLC 2017-07-05 /pmc/articles/PMC5669851/ /pubmed/29137225 http://dx.doi.org/10.18632/oncotarget.18998 Text en Copyright: © 2017 Zou et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Pathology Zou, Meisheng Huang, Chixiong Li, Xinzhong He, Xiang Chen, Yanmei Liao, Wangjun Liao, Yulin Sun, Jie Liu, Ze Zhong, Lintao Bin, Jianping Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection |
title | Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection |
title_full | Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection |
title_fullStr | Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection |
title_full_unstemmed | Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection |
title_short | Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection |
title_sort | circular rna expression profile and potential function of hsa_circrna_101238 in human thoracic aortic dissection |
topic | Research Paper: Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669851/ https://www.ncbi.nlm.nih.gov/pubmed/29137225 http://dx.doi.org/10.18632/oncotarget.18998 |
work_keys_str_mv | AT zoumeisheng circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT huangchixiong circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT lixinzhong circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT hexiang circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT chenyanmei circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT liaowangjun circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT liaoyulin circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT sunjie circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT liuze circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT zhonglintao circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection AT binjianping circularrnaexpressionprofileandpotentialfunctionofhsacircrna101238inhumanthoracicaorticdissection |