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Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection

OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA...

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Autores principales: Zou, Meisheng, Huang, Chixiong, Li, Xinzhong, He, Xiang, Chen, Yanmei, Liao, Wangjun, Liao, Yulin, Sun, Jie, Liu, Ze, Zhong, Lintao, Bin, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669851/
https://www.ncbi.nlm.nih.gov/pubmed/29137225
http://dx.doi.org/10.18632/oncotarget.18998
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author Zou, Meisheng
Huang, Chixiong
Li, Xinzhong
He, Xiang
Chen, Yanmei
Liao, Wangjun
Liao, Yulin
Sun, Jie
Liu, Ze
Zhong, Lintao
Bin, Jianping
author_facet Zou, Meisheng
Huang, Chixiong
Li, Xinzhong
He, Xiang
Chen, Yanmei
Liao, Wangjun
Liao, Yulin
Sun, Jie
Liu, Ze
Zhong, Lintao
Bin, Jianping
author_sort Zou, Meisheng
collection PubMed
description OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA; n=3) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was used to validate the expression pattern of circRNAs, parental genes, and hsa-miR-320a; Western blotting confirmed MMP9 expression with additional samples. Bioinformatic tools including network analysis, Gene ontology, and KEGG pathway analysis were utilized. RESULTS: Among 8,173 detected circRNA genes, 156 upregulated and 106 downregulated significantly in human TAD as compared to NA (P£0.05). Quantitative real-time PCR showed an elevated expression of the upregulated hsa_circRNA_101238, hsa_circRNA_104634, hsa_circRNA_002271, hsa_circRNA_102771, hsa_circRNA_104349, COL1A1, and COL6A3 and reduced of the downregulated hsa_circRNA_102683, hsa_circRNA_005525, hsa_circRNA_103458, and FLNA. Gene ontology analysis revealed that the parental genes favored several pathological processes, such as negative regulation of cell proliferation and extracellular matrix organization. The circRNA-miRNA co-expression network predicted that 33 circRNAs might interact with at least one target miRNAs altered in TAD. KEGG pathway analysis revealed that 28 altered miRNAs were enriched on focal adhesion and vascular smooth muscle contraction. The hsa_circRNA_101238-miRNA-mRNA network indicated the highest degree of hsa-miR-320a. Quantitative real-time PCR and Western blot manifested the low expression of hsa-miR-320a and high of MMP9 in human TAD tissues, respectively. CONCLUSIONS: This study revealed hundreds of differentially expressed circular RNAs in human TAD, suggesting that hsa_circRNA_101238 might inhibit the expression of hsa-miR-320a and increase that of MMP9 in TAD.
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spelling pubmed-56698512017-11-09 Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection Zou, Meisheng Huang, Chixiong Li, Xinzhong He, Xiang Chen, Yanmei Liao, Wangjun Liao, Yulin Sun, Jie Liu, Ze Zhong, Lintao Bin, Jianping Oncotarget Research Paper: Pathology OBJECTIVE: To assess the circular RNAs (circRNAs) expression profile and explore the potential functions in human thoracic aortic dissection (TAD). METHODS: The differentially expressed circRNAs profiles of the aortic segments between human type A TAD patients (n=3) and age-matched normal donors (NA; n=3) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was used to validate the expression pattern of circRNAs, parental genes, and hsa-miR-320a; Western blotting confirmed MMP9 expression with additional samples. Bioinformatic tools including network analysis, Gene ontology, and KEGG pathway analysis were utilized. RESULTS: Among 8,173 detected circRNA genes, 156 upregulated and 106 downregulated significantly in human TAD as compared to NA (P£0.05). Quantitative real-time PCR showed an elevated expression of the upregulated hsa_circRNA_101238, hsa_circRNA_104634, hsa_circRNA_002271, hsa_circRNA_102771, hsa_circRNA_104349, COL1A1, and COL6A3 and reduced of the downregulated hsa_circRNA_102683, hsa_circRNA_005525, hsa_circRNA_103458, and FLNA. Gene ontology analysis revealed that the parental genes favored several pathological processes, such as negative regulation of cell proliferation and extracellular matrix organization. The circRNA-miRNA co-expression network predicted that 33 circRNAs might interact with at least one target miRNAs altered in TAD. KEGG pathway analysis revealed that 28 altered miRNAs were enriched on focal adhesion and vascular smooth muscle contraction. The hsa_circRNA_101238-miRNA-mRNA network indicated the highest degree of hsa-miR-320a. Quantitative real-time PCR and Western blot manifested the low expression of hsa-miR-320a and high of MMP9 in human TAD tissues, respectively. CONCLUSIONS: This study revealed hundreds of differentially expressed circular RNAs in human TAD, suggesting that hsa_circRNA_101238 might inhibit the expression of hsa-miR-320a and increase that of MMP9 in TAD. Impact Journals LLC 2017-07-05 /pmc/articles/PMC5669851/ /pubmed/29137225 http://dx.doi.org/10.18632/oncotarget.18998 Text en Copyright: © 2017 Zou et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Pathology
Zou, Meisheng
Huang, Chixiong
Li, Xinzhong
He, Xiang
Chen, Yanmei
Liao, Wangjun
Liao, Yulin
Sun, Jie
Liu, Ze
Zhong, Lintao
Bin, Jianping
Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
title Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
title_full Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
title_fullStr Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
title_full_unstemmed Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
title_short Circular RNA expression profile and potential function of hsa_circRNA_101238 in human thoracic aortic dissection
title_sort circular rna expression profile and potential function of hsa_circrna_101238 in human thoracic aortic dissection
topic Research Paper: Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669851/
https://www.ncbi.nlm.nih.gov/pubmed/29137225
http://dx.doi.org/10.18632/oncotarget.18998
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