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Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia

BACKGROUND: Cdc42 is a member of the Rho GTPase family and functions as a molecular switch in regulating cytoskeleton remodeling and cell polarity establishment. Inactivating Cdc42 in cardiomyocytes resulted in embryonic lethality with heart developmental defects, including ventricular septum defect...

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Autores principales: Liu, Yang, Wang, Jian, Li, Jieli, Wang, Rui, Tharakan, Binu, Zhang, Shenyuan L., Tong, Carl W., Peng, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670094/
https://www.ncbi.nlm.nih.gov/pubmed/29101495
http://dx.doi.org/10.1186/s40169-017-0171-4
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author Liu, Yang
Wang, Jian
Li, Jieli
Wang, Rui
Tharakan, Binu
Zhang, Shenyuan L.
Tong, Carl W.
Peng, Xu
author_facet Liu, Yang
Wang, Jian
Li, Jieli
Wang, Rui
Tharakan, Binu
Zhang, Shenyuan L.
Tong, Carl W.
Peng, Xu
author_sort Liu, Yang
collection PubMed
description BACKGROUND: Cdc42 is a member of the Rho GTPase family and functions as a molecular switch in regulating cytoskeleton remodeling and cell polarity establishment. Inactivating Cdc42 in cardiomyocytes resulted in embryonic lethality with heart developmental defects, including ventricular septum defects and thin ventricle wall syndrome. FINDINGS: In this study, we have generated a Cdc42 cardiomyocyte knockout mouse line by crossing Cdc42/flox mice with myosin light chain 2a (MLC2a)-Cre mice. We found that the deletion of Cdc42 in embryonic cardiomyocytes resulted in an underdeveloped right ventricle. Microarray analysis and real-time PCR data analysis displayed that the deletion of Cdc42 decreased dHand expression level. In addition, we found evaginations in the ventricle walls of Cdc42 knockout hearts. CONCLUSION: We concluded that Cdc42 plays an essential role in right ventricle growth.
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spelling pubmed-56700942017-11-20 Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia Liu, Yang Wang, Jian Li, Jieli Wang, Rui Tharakan, Binu Zhang, Shenyuan L. Tong, Carl W. Peng, Xu Clin Transl Med Short Report BACKGROUND: Cdc42 is a member of the Rho GTPase family and functions as a molecular switch in regulating cytoskeleton remodeling and cell polarity establishment. Inactivating Cdc42 in cardiomyocytes resulted in embryonic lethality with heart developmental defects, including ventricular septum defects and thin ventricle wall syndrome. FINDINGS: In this study, we have generated a Cdc42 cardiomyocyte knockout mouse line by crossing Cdc42/flox mice with myosin light chain 2a (MLC2a)-Cre mice. We found that the deletion of Cdc42 in embryonic cardiomyocytes resulted in an underdeveloped right ventricle. Microarray analysis and real-time PCR data analysis displayed that the deletion of Cdc42 decreased dHand expression level. In addition, we found evaginations in the ventricle walls of Cdc42 knockout hearts. CONCLUSION: We concluded that Cdc42 plays an essential role in right ventricle growth. Springer Berlin Heidelberg 2017-11-03 /pmc/articles/PMC5670094/ /pubmed/29101495 http://dx.doi.org/10.1186/s40169-017-0171-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Short Report
Liu, Yang
Wang, Jian
Li, Jieli
Wang, Rui
Tharakan, Binu
Zhang, Shenyuan L.
Tong, Carl W.
Peng, Xu
Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
title Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
title_full Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
title_fullStr Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
title_full_unstemmed Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
title_short Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
title_sort deletion of cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670094/
https://www.ncbi.nlm.nih.gov/pubmed/29101495
http://dx.doi.org/10.1186/s40169-017-0171-4
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