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Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR

Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic decisions, as overestimation or underestimation can lead to misguided treatment of a disease or incorrect labelling of the products. Digital PCR is one of the best tools for absolute nucleic-acid copy-number dete...

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Detalles Bibliográficos
Autores principales: Košir, Alexandra Bogožalec, Divieto, Carla, Pavšič, Jernej, Pavarelli, Stefano, Dobnik, David, Dreo, Tanja, Bellotti, Roberto, Sassi, Maria Paola, Žel, Jana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670190/
https://www.ncbi.nlm.nih.gov/pubmed/28921124
http://dx.doi.org/10.1007/s00216-017-0625-y