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Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation

Type 2 diabetes mellitus (T2DM) is characterized by oxidative stress that could lead to chronic micro- and macrovascular complications. We hypothesized that some of the target organ damage is mediated by oxidative alterations in epigenetic mechanisms involving DNA methylation (5mC) and DNA hydroxyme...

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Autores principales: Pinzón-Cortés, Jairo Arturo, Perna-Chaux, Angelina, Rojas-Villamizar, Nicolás Steven, Díaz-Basabe, Angélica, Polanía-Villanueva, Diana Carolina, Jácome, María Fernanda, Mendivil, Carlos Olimpo, Groot, Helena, López-Segura, Valeriano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670276/
https://www.ncbi.nlm.nih.gov/pubmed/28993426
http://dx.doi.org/10.1530/EC-17-0199
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author Pinzón-Cortés, Jairo Arturo
Perna-Chaux, Angelina
Rojas-Villamizar, Nicolás Steven
Díaz-Basabe, Angélica
Polanía-Villanueva, Diana Carolina
Jácome, María Fernanda
Mendivil, Carlos Olimpo
Groot, Helena
López-Segura, Valeriano
author_facet Pinzón-Cortés, Jairo Arturo
Perna-Chaux, Angelina
Rojas-Villamizar, Nicolás Steven
Díaz-Basabe, Angélica
Polanía-Villanueva, Diana Carolina
Jácome, María Fernanda
Mendivil, Carlos Olimpo
Groot, Helena
López-Segura, Valeriano
author_sort Pinzón-Cortés, Jairo Arturo
collection PubMed
description Type 2 diabetes mellitus (T2DM) is characterized by oxidative stress that could lead to chronic micro- and macrovascular complications. We hypothesized that some of the target organ damage is mediated by oxidative alterations in epigenetic mechanisms involving DNA methylation (5mC) and DNA hydroxymethylation (5hmC). We analyzed global DNA methylation and hydroxymethylation in peripheral blood cells in well-controlled and poorly controlled patients with T2DM and compared them with healthy controls. We also analyzed microarrays of DNA methylation and gene expression of other important tissues in the context of diabetes from the GEO database repository and then compared these results with our experimental gene expression data. DNA methylation and, more importantly, DNA hydroxymethylation levels were increased in poorly controlled patients compared to well-controlled and healthy individuals. Both 5mC and 5hmC measurements were correlated with the percentage of glycated hemoglobin, indicating a direct impact of hyperglycemia on changes over the epigenome. The analysis of methylation microarrays was concordant, and 5mC levels were increased in the peripheral blood of T2DM patients. However, the DNA methylation levels were the opposite of those in other tissues, such as the pancreas, adipose tissue and skeletal muscle. We hypothesize that a process of DNA oxidation associated with hyperglycemia may explain the DNA demethylation in which the activity of ten-eleven translocation (TET) proteins is not sufficient to complete the process. High levels of glucose lead to cellular oxidation, which triggers the process of DNA demethylation aided by TET enzymes, resulting in epigenetic dysregulation of the damaged tissues.
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spelling pubmed-56702762017-11-08 Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation Pinzón-Cortés, Jairo Arturo Perna-Chaux, Angelina Rojas-Villamizar, Nicolás Steven Díaz-Basabe, Angélica Polanía-Villanueva, Diana Carolina Jácome, María Fernanda Mendivil, Carlos Olimpo Groot, Helena López-Segura, Valeriano Endocr Connect Research Type 2 diabetes mellitus (T2DM) is characterized by oxidative stress that could lead to chronic micro- and macrovascular complications. We hypothesized that some of the target organ damage is mediated by oxidative alterations in epigenetic mechanisms involving DNA methylation (5mC) and DNA hydroxymethylation (5hmC). We analyzed global DNA methylation and hydroxymethylation in peripheral blood cells in well-controlled and poorly controlled patients with T2DM and compared them with healthy controls. We also analyzed microarrays of DNA methylation and gene expression of other important tissues in the context of diabetes from the GEO database repository and then compared these results with our experimental gene expression data. DNA methylation and, more importantly, DNA hydroxymethylation levels were increased in poorly controlled patients compared to well-controlled and healthy individuals. Both 5mC and 5hmC measurements were correlated with the percentage of glycated hemoglobin, indicating a direct impact of hyperglycemia on changes over the epigenome. The analysis of methylation microarrays was concordant, and 5mC levels were increased in the peripheral blood of T2DM patients. However, the DNA methylation levels were the opposite of those in other tissues, such as the pancreas, adipose tissue and skeletal muscle. We hypothesize that a process of DNA oxidation associated with hyperglycemia may explain the DNA demethylation in which the activity of ten-eleven translocation (TET) proteins is not sufficient to complete the process. High levels of glucose lead to cellular oxidation, which triggers the process of DNA demethylation aided by TET enzymes, resulting in epigenetic dysregulation of the damaged tissues. Bioscientifica Ltd 2017-09-28 /pmc/articles/PMC5670276/ /pubmed/28993426 http://dx.doi.org/10.1530/EC-17-0199 Text en © 2017 The authors http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Research
Pinzón-Cortés, Jairo Arturo
Perna-Chaux, Angelina
Rojas-Villamizar, Nicolás Steven
Díaz-Basabe, Angélica
Polanía-Villanueva, Diana Carolina
Jácome, María Fernanda
Mendivil, Carlos Olimpo
Groot, Helena
López-Segura, Valeriano
Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
title Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
title_full Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
title_fullStr Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
title_full_unstemmed Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
title_short Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation
title_sort effect of diabetes status and hyperglycemia on global dna methylation and hydroxymethylation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670276/
https://www.ncbi.nlm.nih.gov/pubmed/28993426
http://dx.doi.org/10.1530/EC-17-0199
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