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FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis
BACKGROUND: Despite the broad use of FRET techniques, available methods for analyzing protein-protein interaction are subject to high labor and lack of systematic analysis. We propose an open source software allowing the quantitative analysis of fluorescence lifetime imaging (FLIM) while integrating...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670528/ https://www.ncbi.nlm.nih.gov/pubmed/29142589 http://dx.doi.org/10.1186/s13029-017-0067-0 |
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author | Kim, Jiho Tsoy, Yury Persson, Jan Grailhe, Regis |
author_facet | Kim, Jiho Tsoy, Yury Persson, Jan Grailhe, Regis |
author_sort | Kim, Jiho |
collection | PubMed |
description | BACKGROUND: Despite the broad use of FRET techniques, available methods for analyzing protein-protein interaction are subject to high labor and lack of systematic analysis. We propose an open source software allowing the quantitative analysis of fluorescence lifetime imaging (FLIM) while integrating the steady-state fluorescence intensity information for protein-protein interaction studies. FINDINGS: Our developed open source software is dedicated to fluorescence lifetime imaging microscopy (FLIM) data obtained from Becker & Hickl SPC-830. FLIM-FRET analyzer includes: a user-friendly interface enabling automated intensity-based segmentation into single cells, time-resolved fluorescence data fitting to lifetime value for each segmented objects, batch capability, and data representation with donor lifetime versus acceptor/donor intensity quantification as a measure of protein-protein interactions. CONCLUSIONS: The FLIM-FRET analyzer software is a flexible application for lifetime-based FRET analysis. The application, the C#. NET source code, and detailed documentation are freely available at the following URL: http://FLIM-analyzer.ip-korea.org. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13029-017-0067-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5670528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56705282017-11-15 FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis Kim, Jiho Tsoy, Yury Persson, Jan Grailhe, Regis Source Code Biol Med Brief Report BACKGROUND: Despite the broad use of FRET techniques, available methods for analyzing protein-protein interaction are subject to high labor and lack of systematic analysis. We propose an open source software allowing the quantitative analysis of fluorescence lifetime imaging (FLIM) while integrating the steady-state fluorescence intensity information for protein-protein interaction studies. FINDINGS: Our developed open source software is dedicated to fluorescence lifetime imaging microscopy (FLIM) data obtained from Becker & Hickl SPC-830. FLIM-FRET analyzer includes: a user-friendly interface enabling automated intensity-based segmentation into single cells, time-resolved fluorescence data fitting to lifetime value for each segmented objects, batch capability, and data representation with donor lifetime versus acceptor/donor intensity quantification as a measure of protein-protein interactions. CONCLUSIONS: The FLIM-FRET analyzer software is a flexible application for lifetime-based FRET analysis. The application, the C#. NET source code, and detailed documentation are freely available at the following URL: http://FLIM-analyzer.ip-korea.org. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13029-017-0067-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-03 /pmc/articles/PMC5670528/ /pubmed/29142589 http://dx.doi.org/10.1186/s13029-017-0067-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Brief Report Kim, Jiho Tsoy, Yury Persson, Jan Grailhe, Regis FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis |
title | FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis |
title_full | FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis |
title_fullStr | FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis |
title_full_unstemmed | FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis |
title_short | FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis |
title_sort | flim-fret analyzer: open source software for automation of lifetime-based fret analysis |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670528/ https://www.ncbi.nlm.nih.gov/pubmed/29142589 http://dx.doi.org/10.1186/s13029-017-0067-0 |
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