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Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)

BACKGROUND: Plum pox virus (PPV), agent of Sharka disease, is the most important quarantine pathogen of peach (P. persica L. Batsch). Extensive evaluation of peach germplasm has highlighted the lack of resistant sources, while suggesting the presence of a quantitative disease resistance, expressed a...

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Autores principales: Cirilli, Marco, Rossini, Laura, Geuna, Filippo, Palmisano, Francesco, Minafra, Angelantonio, Castrignanò, Tiziana, Gattolin, Stefano, Ciacciulli, Angelo, Babini, Anna Rosa, Liverani, Alessandro, Bassi, Daniele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670703/
https://www.ncbi.nlm.nih.gov/pubmed/29100531
http://dx.doi.org/10.1186/s12870-017-1117-0
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author Cirilli, Marco
Rossini, Laura
Geuna, Filippo
Palmisano, Francesco
Minafra, Angelantonio
Castrignanò, Tiziana
Gattolin, Stefano
Ciacciulli, Angelo
Babini, Anna Rosa
Liverani, Alessandro
Bassi, Daniele
author_facet Cirilli, Marco
Rossini, Laura
Geuna, Filippo
Palmisano, Francesco
Minafra, Angelantonio
Castrignanò, Tiziana
Gattolin, Stefano
Ciacciulli, Angelo
Babini, Anna Rosa
Liverani, Alessandro
Bassi, Daniele
author_sort Cirilli, Marco
collection PubMed
description BACKGROUND: Plum pox virus (PPV), agent of Sharka disease, is the most important quarantine pathogen of peach (P. persica L. Batsch). Extensive evaluation of peach germplasm has highlighted the lack of resistant sources, while suggesting the presence of a quantitative disease resistance, expressed as reduction in the intensity of symptoms. Unravelling the genetic architecture of peach response to PPV infection is essential for pyramiding resistant genes and for developing more tolerant varieties. For this purpose, a genome-wide association (GWA) approach was applied in a panel of accessions phenotyped for virus susceptibility and genotyped with the IPSC peach 9 K SNP Array, and coupled with an high-coverage resequencing of the tolerant accession ‘Kamarat’. RESULTS: Genome-wide association identified three highly significant associated loci on chromosome 2 and 3, accounting for most of the reduction in PPV-M susceptibility within the analysed peach population. The exploration of associated intervals through whole-genome comparison of the tolerant accession ‘Kamarat’ and other susceptible accessions, including the PPV-resistant wild-related species P. davidiana, allow the identification of allelic variants in promising candidate genes, including an RTM2-like gene already characterized in A. thaliana. CONCLUSIONS: The present study is the first effort to identify genetic factors involved in Sharka disease in peach germplasm through a GWA approach. We provide evidence of the presence of quantitative resistant loci in a collection of peach accessions, identifying major loci and highly informative SNPs that could be useful for marker assisted selection. These results could serve as reference bases for future research aimed at the comprehension of genetic mechanism regulating the complex peach-PPV interaction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-017-1117-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-56707032017-11-15 Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch) Cirilli, Marco Rossini, Laura Geuna, Filippo Palmisano, Francesco Minafra, Angelantonio Castrignanò, Tiziana Gattolin, Stefano Ciacciulli, Angelo Babini, Anna Rosa Liverani, Alessandro Bassi, Daniele BMC Plant Biol Research Article BACKGROUND: Plum pox virus (PPV), agent of Sharka disease, is the most important quarantine pathogen of peach (P. persica L. Batsch). Extensive evaluation of peach germplasm has highlighted the lack of resistant sources, while suggesting the presence of a quantitative disease resistance, expressed as reduction in the intensity of symptoms. Unravelling the genetic architecture of peach response to PPV infection is essential for pyramiding resistant genes and for developing more tolerant varieties. For this purpose, a genome-wide association (GWA) approach was applied in a panel of accessions phenotyped for virus susceptibility and genotyped with the IPSC peach 9 K SNP Array, and coupled with an high-coverage resequencing of the tolerant accession ‘Kamarat’. RESULTS: Genome-wide association identified three highly significant associated loci on chromosome 2 and 3, accounting for most of the reduction in PPV-M susceptibility within the analysed peach population. The exploration of associated intervals through whole-genome comparison of the tolerant accession ‘Kamarat’ and other susceptible accessions, including the PPV-resistant wild-related species P. davidiana, allow the identification of allelic variants in promising candidate genes, including an RTM2-like gene already characterized in A. thaliana. CONCLUSIONS: The present study is the first effort to identify genetic factors involved in Sharka disease in peach germplasm through a GWA approach. We provide evidence of the presence of quantitative resistant loci in a collection of peach accessions, identifying major loci and highly informative SNPs that could be useful for marker assisted selection. These results could serve as reference bases for future research aimed at the comprehension of genetic mechanism regulating the complex peach-PPV interaction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-017-1117-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-03 /pmc/articles/PMC5670703/ /pubmed/29100531 http://dx.doi.org/10.1186/s12870-017-1117-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cirilli, Marco
Rossini, Laura
Geuna, Filippo
Palmisano, Francesco
Minafra, Angelantonio
Castrignanò, Tiziana
Gattolin, Stefano
Ciacciulli, Angelo
Babini, Anna Rosa
Liverani, Alessandro
Bassi, Daniele
Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)
title Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)
title_full Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)
title_fullStr Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)
title_full_unstemmed Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)
title_short Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)
title_sort genetic dissection of sharka disease tolerance in peach (p. persica l. batsch)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670703/
https://www.ncbi.nlm.nih.gov/pubmed/29100531
http://dx.doi.org/10.1186/s12870-017-1117-0
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