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Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood

BACKGROUND: Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglec...

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Autores principales: Mehlotra, Rajeev K., Blankenship, D’Arbra, Howes, Rosalind E., Rakotomanga, Tovonahary A., Ramiranirina, Brune, Ramboarina, Stephanie, Franchard, Thierry, Linger, Marlin H., Zikursh-Blood, Melinda, Ratsimbasoa, Arsène C., Zimmerman, Peter A., Grimberg, Brian T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670718/
https://www.ncbi.nlm.nih.gov/pubmed/29100506
http://dx.doi.org/10.1186/s12936-017-2090-7
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author Mehlotra, Rajeev K.
Blankenship, D’Arbra
Howes, Rosalind E.
Rakotomanga, Tovonahary A.
Ramiranirina, Brune
Ramboarina, Stephanie
Franchard, Thierry
Linger, Marlin H.
Zikursh-Blood, Melinda
Ratsimbasoa, Arsène C.
Zimmerman, Peter A.
Grimberg, Brian T.
author_facet Mehlotra, Rajeev K.
Blankenship, D’Arbra
Howes, Rosalind E.
Rakotomanga, Tovonahary A.
Ramiranirina, Brune
Ramboarina, Stephanie
Franchard, Thierry
Linger, Marlin H.
Zikursh-Blood, Melinda
Ratsimbasoa, Arsène C.
Zimmerman, Peter A.
Grimberg, Brian T.
author_sort Mehlotra, Rajeev K.
collection PubMed
description BACKGROUND: Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglected and under-studied tropical disease. Significant challenges bringing P. vivax into the laboratory, particularly the capacity for long-term propagation of well-characterized strains, have limited the study of this parasite’s red blood cell (RBC) invasion mechanism, blood-stage development, gene expression, and genetic manipulation. METHODS AND RESULTS: Patient isolates of P. vivax have been collected and cryopreserved in the rural community of Ampasimpotsy, located in the Tsiroanomandidy Health District of Madagascar. Periodic, monthly overland transport of these cryopreserved isolates to the country’s National Malaria Control Programme laboratory in Antananarivo preceded onward sample transfer to laboratories at Case Western Reserve University, USA. There, the P. vivax isolates have been cultured through propagation in the RBCs of Saimiri boliviensis. For the four patient isolates studied to-date, the median time interval between sample collection and in vitro culture has been 454 days (range 166–961 days). The median time in culture, continually documented by light microscopy, has been 159 days; isolate AMP2014.01 was continuously propagated for 233 days. Further studies show that the P. vivax parasites propagated in Saimiri RBCs retain their ability to invade human RBCs, and can be cryopreserved, thawed and successfully returned to productive in vitro culture. CONCLUSIONS/SIGNIFICANCE: Long-term culture of P. vivax is possible in the RBCs of Saimiri boliviensis. These studies provide an alternative to propagation of P. vivax in live animals that are becoming more restricted. In vitro culture of P. vivax in Saimiri RBCs provides an opening to stabilize patient isolates, which would serve as precious resources to apply new strategies for investigating the molecular and cellular biology of this important malaria parasite. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12936-017-2090-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-56707182017-11-15 Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood Mehlotra, Rajeev K. Blankenship, D’Arbra Howes, Rosalind E. Rakotomanga, Tovonahary A. Ramiranirina, Brune Ramboarina, Stephanie Franchard, Thierry Linger, Marlin H. Zikursh-Blood, Melinda Ratsimbasoa, Arsène C. Zimmerman, Peter A. Grimberg, Brian T. Malar J Research BACKGROUND: Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglected and under-studied tropical disease. Significant challenges bringing P. vivax into the laboratory, particularly the capacity for long-term propagation of well-characterized strains, have limited the study of this parasite’s red blood cell (RBC) invasion mechanism, blood-stage development, gene expression, and genetic manipulation. METHODS AND RESULTS: Patient isolates of P. vivax have been collected and cryopreserved in the rural community of Ampasimpotsy, located in the Tsiroanomandidy Health District of Madagascar. Periodic, monthly overland transport of these cryopreserved isolates to the country’s National Malaria Control Programme laboratory in Antananarivo preceded onward sample transfer to laboratories at Case Western Reserve University, USA. There, the P. vivax isolates have been cultured through propagation in the RBCs of Saimiri boliviensis. For the four patient isolates studied to-date, the median time interval between sample collection and in vitro culture has been 454 days (range 166–961 days). The median time in culture, continually documented by light microscopy, has been 159 days; isolate AMP2014.01 was continuously propagated for 233 days. Further studies show that the P. vivax parasites propagated in Saimiri RBCs retain their ability to invade human RBCs, and can be cryopreserved, thawed and successfully returned to productive in vitro culture. CONCLUSIONS/SIGNIFICANCE: Long-term culture of P. vivax is possible in the RBCs of Saimiri boliviensis. These studies provide an alternative to propagation of P. vivax in live animals that are becoming more restricted. In vitro culture of P. vivax in Saimiri RBCs provides an opening to stabilize patient isolates, which would serve as precious resources to apply new strategies for investigating the molecular and cellular biology of this important malaria parasite. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12936-017-2090-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-03 /pmc/articles/PMC5670718/ /pubmed/29100506 http://dx.doi.org/10.1186/s12936-017-2090-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Mehlotra, Rajeev K.
Blankenship, D’Arbra
Howes, Rosalind E.
Rakotomanga, Tovonahary A.
Ramiranirina, Brune
Ramboarina, Stephanie
Franchard, Thierry
Linger, Marlin H.
Zikursh-Blood, Melinda
Ratsimbasoa, Arsène C.
Zimmerman, Peter A.
Grimberg, Brian T.
Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
title Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
title_full Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
title_fullStr Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
title_full_unstemmed Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
title_short Long-term in vitro culture of Plasmodium vivax isolates from Madagascar maintained in Saimiri boliviensis blood
title_sort long-term in vitro culture of plasmodium vivax isolates from madagascar maintained in saimiri boliviensis blood
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5670718/
https://www.ncbi.nlm.nih.gov/pubmed/29100506
http://dx.doi.org/10.1186/s12936-017-2090-7
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