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The Role of Cholecystokinin in Peripheral Taste Signaling in Mice
Cholecystokinin (CCK) is a gut hormone released from enteroendocrine cells. CCK functions as an anorexigenic factor by acting on CCK receptors expressed on the vagal afferent nerve and hypothalamus with a synergistic interaction between leptin. In the gut, tastants such as amino acids and bitter com...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671461/ https://www.ncbi.nlm.nih.gov/pubmed/29163209 http://dx.doi.org/10.3389/fphys.2017.00866 |
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author | Yoshida, Ryusuke Shin, Misa Yasumatsu, Keiko Takai, Shingo Inoue, Mayuko Shigemura, Noriatsu Takiguchi, Soichi Nakamura, Seiji Ninomiya, Yuzo |
author_facet | Yoshida, Ryusuke Shin, Misa Yasumatsu, Keiko Takai, Shingo Inoue, Mayuko Shigemura, Noriatsu Takiguchi, Soichi Nakamura, Seiji Ninomiya, Yuzo |
author_sort | Yoshida, Ryusuke |
collection | PubMed |
description | Cholecystokinin (CCK) is a gut hormone released from enteroendocrine cells. CCK functions as an anorexigenic factor by acting on CCK receptors expressed on the vagal afferent nerve and hypothalamus with a synergistic interaction between leptin. In the gut, tastants such as amino acids and bitter compounds stimulate CCK release from enteroendocrine cells via activation of taste transduction pathways. CCK is also expressed in taste buds, suggesting potential roles of CCK in taste signaling in the peripheral taste organ. In the present study, we focused on the function of CCK in the initial responses to taste stimulation. CCK was coexpressed with type II taste cell markers such as Gα-gustducin, phospholipase Cβ2, and transient receptor potential channel M5. Furthermore, a small subset (~30%) of CCK-expressing taste cells expressed a sweet/umami taste receptor component, taste receptor type 1 member 3, in taste buds. Because type II taste cells are sweet, umami or bitter taste cells, the majority of CCK-expressing taste cells may be bitter taste cells. CCK-A and -B receptors were expressed in both taste cells and gustatory neurons. CCK receptor knockout mice showed reduced neural responses to bitter compounds compared with wild-type mice. Consistently, intravenous injection of CCK-Ar antagonist lorglumide selectively suppressed gustatory nerve responses to bitter compounds. Intravenous injection of CCK-8 transiently increased gustatory nerve activities in a dose-dependent manner whereas administration of CCK-8 did not affect activities of bitter-sensitive taste cells. Collectively, CCK may be a functionally important neurotransmitter or neuromodulator to activate bitter nerve fibers in peripheral taste tissues. |
format | Online Article Text |
id | pubmed-5671461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56714612017-11-21 The Role of Cholecystokinin in Peripheral Taste Signaling in Mice Yoshida, Ryusuke Shin, Misa Yasumatsu, Keiko Takai, Shingo Inoue, Mayuko Shigemura, Noriatsu Takiguchi, Soichi Nakamura, Seiji Ninomiya, Yuzo Front Physiol Physiology Cholecystokinin (CCK) is a gut hormone released from enteroendocrine cells. CCK functions as an anorexigenic factor by acting on CCK receptors expressed on the vagal afferent nerve and hypothalamus with a synergistic interaction between leptin. In the gut, tastants such as amino acids and bitter compounds stimulate CCK release from enteroendocrine cells via activation of taste transduction pathways. CCK is also expressed in taste buds, suggesting potential roles of CCK in taste signaling in the peripheral taste organ. In the present study, we focused on the function of CCK in the initial responses to taste stimulation. CCK was coexpressed with type II taste cell markers such as Gα-gustducin, phospholipase Cβ2, and transient receptor potential channel M5. Furthermore, a small subset (~30%) of CCK-expressing taste cells expressed a sweet/umami taste receptor component, taste receptor type 1 member 3, in taste buds. Because type II taste cells are sweet, umami or bitter taste cells, the majority of CCK-expressing taste cells may be bitter taste cells. CCK-A and -B receptors were expressed in both taste cells and gustatory neurons. CCK receptor knockout mice showed reduced neural responses to bitter compounds compared with wild-type mice. Consistently, intravenous injection of CCK-Ar antagonist lorglumide selectively suppressed gustatory nerve responses to bitter compounds. Intravenous injection of CCK-8 transiently increased gustatory nerve activities in a dose-dependent manner whereas administration of CCK-8 did not affect activities of bitter-sensitive taste cells. Collectively, CCK may be a functionally important neurotransmitter or neuromodulator to activate bitter nerve fibers in peripheral taste tissues. Frontiers Media S.A. 2017-10-31 /pmc/articles/PMC5671461/ /pubmed/29163209 http://dx.doi.org/10.3389/fphys.2017.00866 Text en Copyright © 2017 Yoshida, Shin, Yasumatsu, Takai, Inoue, Shigemura, Takiguchi, Nakamura and Ninomiya. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Yoshida, Ryusuke Shin, Misa Yasumatsu, Keiko Takai, Shingo Inoue, Mayuko Shigemura, Noriatsu Takiguchi, Soichi Nakamura, Seiji Ninomiya, Yuzo The Role of Cholecystokinin in Peripheral Taste Signaling in Mice |
title | The Role of Cholecystokinin in Peripheral Taste Signaling in Mice |
title_full | The Role of Cholecystokinin in Peripheral Taste Signaling in Mice |
title_fullStr | The Role of Cholecystokinin in Peripheral Taste Signaling in Mice |
title_full_unstemmed | The Role of Cholecystokinin in Peripheral Taste Signaling in Mice |
title_short | The Role of Cholecystokinin in Peripheral Taste Signaling in Mice |
title_sort | role of cholecystokinin in peripheral taste signaling in mice |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671461/ https://www.ncbi.nlm.nih.gov/pubmed/29163209 http://dx.doi.org/10.3389/fphys.2017.00866 |
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