Cargando…
The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System
During pathogenic infection, hundreds of proteins that play vital roles in the Verticillium dahliae-host interaction are secreted. In this study, an integrated proteomic analysis of secreted V. dahliae proteins was performed, and a conserved secretory protein, designated VdCP1, was identified as a m...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671667/ https://www.ncbi.nlm.nih.gov/pubmed/29163605 http://dx.doi.org/10.3389/fpls.2017.01880 |
_version_ | 1783276283306704896 |
---|---|
author | Zhang, Yi Gao, Yuhan Liang, Yingbo Dong, Yijie Yang, Xiufen Yuan, Jingjing Qiu, Dewen |
author_facet | Zhang, Yi Gao, Yuhan Liang, Yingbo Dong, Yijie Yang, Xiufen Yuan, Jingjing Qiu, Dewen |
author_sort | Zhang, Yi |
collection | PubMed |
description | During pathogenic infection, hundreds of proteins that play vital roles in the Verticillium dahliae-host interaction are secreted. In this study, an integrated proteomic analysis of secreted V. dahliae proteins was performed, and a conserved secretory protein, designated VdCP1, was identified as a member of the SnodProt1 phytotoxin family. An expression analysis of the vdcp1 gene revealed that the transcript is present in every condition studied and displays elevated expression throughout the infection process. To investigate the natural role of VdCP1 in V. dahliae, two vdcp1 knockout mutants and their complementation strains were generated. Bioassays of these mutants revealed no obvious phenotypic differences from the wild-type (WT) in terms of mycelial growth, conidial production or mycelial/spore morphology. However, compared with the WT, the vdcp1 knockout mutants displayed attenuated pathogenicity in cotton plants. Furthermore, treating plants with purified recombinant VdCP1 protein expressed in Pichia pastoris induced the accumulation of reactive oxygen species (ROS), expression of several defense-related genes, leakage of ion electrolytes, enhancement of defense-related enzyme activity and production of salicylic acid. Moreover, VdCP1 conferred resistance to Botrytis cinerea and Pseudomonas syringae pv. tabaci in tobacco and to V. dahliae in cotton. Further research revealed that VdCP1 possesses chitin-binding properties and that the growth of vdcp1 knockout mutants was more affected by treatments with chitinase, indicating that VdCP1 could protect V. dahliae cell wall from enzymatic degradation, which suggests an effector role of VdCP1 in infecting hosts. |
format | Online Article Text |
id | pubmed-5671667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56716672017-11-21 The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System Zhang, Yi Gao, Yuhan Liang, Yingbo Dong, Yijie Yang, Xiufen Yuan, Jingjing Qiu, Dewen Front Plant Sci Plant Science During pathogenic infection, hundreds of proteins that play vital roles in the Verticillium dahliae-host interaction are secreted. In this study, an integrated proteomic analysis of secreted V. dahliae proteins was performed, and a conserved secretory protein, designated VdCP1, was identified as a member of the SnodProt1 phytotoxin family. An expression analysis of the vdcp1 gene revealed that the transcript is present in every condition studied and displays elevated expression throughout the infection process. To investigate the natural role of VdCP1 in V. dahliae, two vdcp1 knockout mutants and their complementation strains were generated. Bioassays of these mutants revealed no obvious phenotypic differences from the wild-type (WT) in terms of mycelial growth, conidial production or mycelial/spore morphology. However, compared with the WT, the vdcp1 knockout mutants displayed attenuated pathogenicity in cotton plants. Furthermore, treating plants with purified recombinant VdCP1 protein expressed in Pichia pastoris induced the accumulation of reactive oxygen species (ROS), expression of several defense-related genes, leakage of ion electrolytes, enhancement of defense-related enzyme activity and production of salicylic acid. Moreover, VdCP1 conferred resistance to Botrytis cinerea and Pseudomonas syringae pv. tabaci in tobacco and to V. dahliae in cotton. Further research revealed that VdCP1 possesses chitin-binding properties and that the growth of vdcp1 knockout mutants was more affected by treatments with chitinase, indicating that VdCP1 could protect V. dahliae cell wall from enzymatic degradation, which suggests an effector role of VdCP1 in infecting hosts. Frontiers Media S.A. 2017-10-31 /pmc/articles/PMC5671667/ /pubmed/29163605 http://dx.doi.org/10.3389/fpls.2017.01880 Text en Copyright © 2017 Zhang, Gao, Liang, Dong, Yang, Yuan and Qiu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Yi Gao, Yuhan Liang, Yingbo Dong, Yijie Yang, Xiufen Yuan, Jingjing Qiu, Dewen The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System |
title | The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System |
title_full | The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System |
title_fullStr | The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System |
title_full_unstemmed | The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System |
title_short | The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System |
title_sort | verticillium dahliae snodprot1-like protein vdcp1 contributes to virulence and triggers the plant immune system |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671667/ https://www.ncbi.nlm.nih.gov/pubmed/29163605 http://dx.doi.org/10.3389/fpls.2017.01880 |
work_keys_str_mv | AT zhangyi theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT gaoyuhan theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT liangyingbo theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT dongyijie theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT yangxiufen theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT yuanjingjing theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT qiudewen theverticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT zhangyi verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT gaoyuhan verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT liangyingbo verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT dongyijie verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT yangxiufen verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT yuanjingjing verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem AT qiudewen verticilliumdahliaesnodprot1likeproteinvdcp1contributestovirulenceandtriggerstheplantimmunesystem |