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Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs

It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates the...

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Autores principales: Bit, Arindam, Kumar, Awanish, Singh, Abhishek Kumar, Rizvanov, Albert A., Kiassov, Andrey P., Patra, Pradeep Kumar, Kumar, Munish, Bissoyi, Akalabya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5672132/
https://www.ncbi.nlm.nih.gov/pubmed/29201057
http://dx.doi.org/10.1155/2017/1380304
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author Bit, Arindam
Kumar, Awanish
Singh, Abhishek Kumar
Rizvanov, Albert A.
Kiassov, Andrey P.
Patra, Pradeep Kumar
Kumar, Munish
Bissoyi, Akalabya
author_facet Bit, Arindam
Kumar, Awanish
Singh, Abhishek Kumar
Rizvanov, Albert A.
Kiassov, Andrey P.
Patra, Pradeep Kumar
Kumar, Munish
Bissoyi, Akalabya
author_sort Bit, Arindam
collection PubMed
description It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues.
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spelling pubmed-56721322017-12-03 Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs Bit, Arindam Kumar, Awanish Singh, Abhishek Kumar Rizvanov, Albert A. Kiassov, Andrey P. Patra, Pradeep Kumar Kumar, Munish Bissoyi, Akalabya Stem Cells Int Review Article It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues. Hindawi 2017 2017-10-19 /pmc/articles/PMC5672132/ /pubmed/29201057 http://dx.doi.org/10.1155/2017/1380304 Text en Copyright © 2017 Arindam Bit et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Bit, Arindam
Kumar, Awanish
Singh, Abhishek Kumar
Rizvanov, Albert A.
Kiassov, Andrey P.
Patra, Pradeep Kumar
Kumar, Munish
Bissoyi, Akalabya
Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
title Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
title_full Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
title_fullStr Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
title_full_unstemmed Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
title_short Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs
title_sort crosstalk between substrates and rho-associated kinase inhibitors in cryopreservation of tissue-engineered constructs
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5672132/
https://www.ncbi.nlm.nih.gov/pubmed/29201057
http://dx.doi.org/10.1155/2017/1380304
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