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A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)

Abstract. An account is given of the karyotypes and male meiosis of the Water Scorpion Nepa cinerea Linnaeus, 1758 and the Water Stick Insect Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepomorpha, Nepidae). A number of different approaches and techniques were tried: the employment of both male...

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Autores principales: Angus, Robert B., Jeangirard, Constance, Stoianova, Desislava, Grozeva, Snejana, Kuznetsova, Valentina G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5672273/
https://www.ncbi.nlm.nih.gov/pubmed/29114353
http://dx.doi.org/10.3897/CompCytogen.v11i4.14928
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author Angus, Robert B.
Jeangirard, Constance
Stoianova, Desislava
Grozeva, Snejana
Kuznetsova, Valentina G.
author_facet Angus, Robert B.
Jeangirard, Constance
Stoianova, Desislava
Grozeva, Snejana
Kuznetsova, Valentina G.
author_sort Angus, Robert B.
collection PubMed
description Abstract. An account is given of the karyotypes and male meiosis of the Water Scorpion Nepa cinerea Linnaeus, 1758 and the Water Stick Insect Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepomorpha, Nepidae). A number of different approaches and techniques were tried: the employment of both male and female gonads and mid-guts as the sources of chromosomes, squash and air-drying methods for chromosome preparations, C-banding and fluorescence in situ hybridization (FISH) for chromosome study. We found that N. cinerea had a karyotype comprising 14 pairs of autosomes and a multiple sex chromosome system, which is X(1)X(2)X(3)X(4)Y (♂) / X(1)X(1)X(2)X(2)X(3)X(3)X(4)X(4) (♀), whereas R. linearis had a karyotype comprising 19 pairs of autosomes and a multiple sex chromosome system X(1)X(2)X(3)X(4)Y (♂) / X(1)X(1)X(2)X(2)X(3)X(3)X(4)X(4) (♀). In both N. cinerea and R. linearis, the autosomes formed chiasmate bivalents in spermatogenesis, and the sex chromosome univalents divided during the first meiotic division and segregated during the second one suggesting thus a post-reductional type of behaviour. These results confirm and amplify those of Steopoe (1925, 1927, 1931, 1932) but are inconsistent with those of other researchers. C-banding appeared helpful in pairing up the autosomes for karyotype assembly; however in R. linearis the chromosomes were much more uniform in size and general appearance than in N. cinerea. FISH for 18S ribosomal DNA (major rDNA) revealed hybridization signals on two of the five sex chromosomes in N. cinerea. In R. linearis, rDNA location was less obvious than in N. cinerea; however it is suggested to be similar. We have detected the presence of the canonical “insect” (TTAGG)(n) telomeric repeat in chromosomes of these species. This is the first application of C-banding and FISH in the family Nepidae.
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spelling pubmed-56722732017-11-07 A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae) Angus, Robert B. Jeangirard, Constance Stoianova, Desislava Grozeva, Snejana Kuznetsova, Valentina G. Comp Cytogenet Research Article Abstract. An account is given of the karyotypes and male meiosis of the Water Scorpion Nepa cinerea Linnaeus, 1758 and the Water Stick Insect Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepomorpha, Nepidae). A number of different approaches and techniques were tried: the employment of both male and female gonads and mid-guts as the sources of chromosomes, squash and air-drying methods for chromosome preparations, C-banding and fluorescence in situ hybridization (FISH) for chromosome study. We found that N. cinerea had a karyotype comprising 14 pairs of autosomes and a multiple sex chromosome system, which is X(1)X(2)X(3)X(4)Y (♂) / X(1)X(1)X(2)X(2)X(3)X(3)X(4)X(4) (♀), whereas R. linearis had a karyotype comprising 19 pairs of autosomes and a multiple sex chromosome system X(1)X(2)X(3)X(4)Y (♂) / X(1)X(1)X(2)X(2)X(3)X(3)X(4)X(4) (♀). In both N. cinerea and R. linearis, the autosomes formed chiasmate bivalents in spermatogenesis, and the sex chromosome univalents divided during the first meiotic division and segregated during the second one suggesting thus a post-reductional type of behaviour. These results confirm and amplify those of Steopoe (1925, 1927, 1931, 1932) but are inconsistent with those of other researchers. C-banding appeared helpful in pairing up the autosomes for karyotype assembly; however in R. linearis the chromosomes were much more uniform in size and general appearance than in N. cinerea. FISH for 18S ribosomal DNA (major rDNA) revealed hybridization signals on two of the five sex chromosomes in N. cinerea. In R. linearis, rDNA location was less obvious than in N. cinerea; however it is suggested to be similar. We have detected the presence of the canonical “insect” (TTAGG)(n) telomeric repeat in chromosomes of these species. This is the first application of C-banding and FISH in the family Nepidae. Pensoft Publishers 2017-09-14 /pmc/articles/PMC5672273/ /pubmed/29114353 http://dx.doi.org/10.3897/CompCytogen.v11i4.14928 Text en Robert B. Angus, Constance Jeangirard, Desislava Stoianova, Snejana Grozeva, Valentina G. Kuznetsova http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Angus, Robert B.
Jeangirard, Constance
Stoianova, Desislava
Grozeva, Snejana
Kuznetsova, Valentina G.
A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)
title A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)
title_full A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)
title_fullStr A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)
title_full_unstemmed A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)
title_short A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)
title_sort chromosomal analysis of nepa cinerea linnaeus, 1758 and ranatra linearis (linnaeus, 1758) (heteroptera, nepidae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5672273/
https://www.ncbi.nlm.nih.gov/pubmed/29114353
http://dx.doi.org/10.3897/CompCytogen.v11i4.14928
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