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The trophoblast survival capacity in preeclampsia

BACKGROUND: Preeclampsia has become the world’s major maternal health problem putting a huge burden on mothers, newborns and also on the health systems. The pathogenesis of preeclampsia seems to include events in very early pregnancy affecting differentiation of placental villous trophoblast. The ar...

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Autores principales: Hutabarat, Martina, Wibowo, Noroyono, Huppertz, Berthold
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673174/
https://www.ncbi.nlm.nih.gov/pubmed/29107968
http://dx.doi.org/10.1371/journal.pone.0186909
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author Hutabarat, Martina
Wibowo, Noroyono
Huppertz, Berthold
author_facet Hutabarat, Martina
Wibowo, Noroyono
Huppertz, Berthold
author_sort Hutabarat, Martina
collection PubMed
description BACKGROUND: Preeclampsia has become the world’s major maternal health problem putting a huge burden on mothers, newborns and also on the health systems. The pathogenesis of preeclampsia seems to include events in very early pregnancy affecting differentiation of placental villous trophoblast. The arising changes of the cell death spectrum from apoptosis via increased autophagy and aponecrosis to necrosis in turn induce systemic inflammation of the mother. METHODS: Placental tissue samples and maternal serum samples from 40 pregnant women were collected from normal pregnancy, IUGR, early-onset and late-onset preeclampsia. Immunohistochemistry for LC3B and Beclin-1 was quantified using systematic random sampling techniques. Serum levels of LDH and other markers were assessed in serum. RESULTS: Expression of the autophagy markers LC3B and Beclin-1 was significantly different between groups as was the LC3B/Beclin-1 ratio. Early-onset preeclampsia and IUGR had the highest autophagy protein expression levels, while normal pregnancy and late-onset preeclampsia had the highest LC3B/Beclin-1 ratio. Early-onset preeclampsia had the highest negative correlation with free LDH as cell defect marker. CONCLUSIONS: Autophagy plays a critical role in the cell death spectrum and cellular survival capacity of villous trophoblast. Alterations in autophagic protein expression are involved in pathological pregnancies such as preeclampsia.
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spelling pubmed-56731742017-11-18 The trophoblast survival capacity in preeclampsia Hutabarat, Martina Wibowo, Noroyono Huppertz, Berthold PLoS One Research Article BACKGROUND: Preeclampsia has become the world’s major maternal health problem putting a huge burden on mothers, newborns and also on the health systems. The pathogenesis of preeclampsia seems to include events in very early pregnancy affecting differentiation of placental villous trophoblast. The arising changes of the cell death spectrum from apoptosis via increased autophagy and aponecrosis to necrosis in turn induce systemic inflammation of the mother. METHODS: Placental tissue samples and maternal serum samples from 40 pregnant women were collected from normal pregnancy, IUGR, early-onset and late-onset preeclampsia. Immunohistochemistry for LC3B and Beclin-1 was quantified using systematic random sampling techniques. Serum levels of LDH and other markers were assessed in serum. RESULTS: Expression of the autophagy markers LC3B and Beclin-1 was significantly different between groups as was the LC3B/Beclin-1 ratio. Early-onset preeclampsia and IUGR had the highest autophagy protein expression levels, while normal pregnancy and late-onset preeclampsia had the highest LC3B/Beclin-1 ratio. Early-onset preeclampsia had the highest negative correlation with free LDH as cell defect marker. CONCLUSIONS: Autophagy plays a critical role in the cell death spectrum and cellular survival capacity of villous trophoblast. Alterations in autophagic protein expression are involved in pathological pregnancies such as preeclampsia. Public Library of Science 2017-11-06 /pmc/articles/PMC5673174/ /pubmed/29107968 http://dx.doi.org/10.1371/journal.pone.0186909 Text en © 2017 Hutabarat et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hutabarat, Martina
Wibowo, Noroyono
Huppertz, Berthold
The trophoblast survival capacity in preeclampsia
title The trophoblast survival capacity in preeclampsia
title_full The trophoblast survival capacity in preeclampsia
title_fullStr The trophoblast survival capacity in preeclampsia
title_full_unstemmed The trophoblast survival capacity in preeclampsia
title_short The trophoblast survival capacity in preeclampsia
title_sort trophoblast survival capacity in preeclampsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673174/
https://www.ncbi.nlm.nih.gov/pubmed/29107968
http://dx.doi.org/10.1371/journal.pone.0186909
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