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A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis

OBJECTIVE(S): Tuberculosis (TB) has still remained a global health issue. One third of the world’s population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the eff...

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Autores principales: Teimourpour, Roghayeh, peeridogaheh, Hadi, Teimourpour, Amir, Arzanlou, Mohsen, Meshkat, Zahra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673696/
https://www.ncbi.nlm.nih.gov/pubmed/29147487
http://dx.doi.org/10.22038/IJBMS.2017.9445
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author Teimourpour, Roghayeh
peeridogaheh, Hadi
Teimourpour, Amir
Arzanlou, Mohsen
Meshkat, Zahra
author_facet Teimourpour, Roghayeh
peeridogaheh, Hadi
Teimourpour, Amir
Arzanlou, Mohsen
Meshkat, Zahra
author_sort Teimourpour, Roghayeh
collection PubMed
description OBJECTIVE(S): Tuberculosis (TB) has still remained a global health issue. One third of the world’s population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses against Mycobacterium tuberculosis. MATERIALS AND METHODS: A DNA plasmid vaccine expressing Mtb32C-HBHA fusion protein was constructed and its ability in protein expression was examined by RT-PCR and Western blot methods. Female BALB/c mice were vaccinated with 100 μg of purified recombinant vector in an attempt to assess its immunogenicity and protective efficacy. Further, the cytokines, IFN-γ, IL-12, IL-4, IL-10, and TGF-β were assessed. RESULTS: The levels of all the studied cytokines were significantly increased (P<0.05) compared with the control group. IFN-γ production in the group receiving DNA vaccine plus BCG was increased compared with those receiving only DNA vaccine or BCG (P<0.001). CONCLUSION: The immunogenicity of the new chimeric DNA vaccine was confirmed alone and in combination with BCG. Based on the results of the current study, the constructed DNA vaccine induced the expression of Mtb32C-HBHA fusion protein efficiently in vitro. Furthermore, high levels of the specific cytokines were induced in mice. By using this DNA vaccine as a booster after BCG, higher amounts of IFN-γ will be produced.
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spelling pubmed-56736962017-11-16 A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis Teimourpour, Roghayeh peeridogaheh, Hadi Teimourpour, Amir Arzanlou, Mohsen Meshkat, Zahra Iran J Basic Med Sci Original Article OBJECTIVE(S): Tuberculosis (TB) has still remained a global health issue. One third of the world’s population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses against Mycobacterium tuberculosis. MATERIALS AND METHODS: A DNA plasmid vaccine expressing Mtb32C-HBHA fusion protein was constructed and its ability in protein expression was examined by RT-PCR and Western blot methods. Female BALB/c mice were vaccinated with 100 μg of purified recombinant vector in an attempt to assess its immunogenicity and protective efficacy. Further, the cytokines, IFN-γ, IL-12, IL-4, IL-10, and TGF-β were assessed. RESULTS: The levels of all the studied cytokines were significantly increased (P<0.05) compared with the control group. IFN-γ production in the group receiving DNA vaccine plus BCG was increased compared with those receiving only DNA vaccine or BCG (P<0.001). CONCLUSION: The immunogenicity of the new chimeric DNA vaccine was confirmed alone and in combination with BCG. Based on the results of the current study, the constructed DNA vaccine induced the expression of Mtb32C-HBHA fusion protein efficiently in vitro. Furthermore, high levels of the specific cytokines were induced in mice. By using this DNA vaccine as a booster after BCG, higher amounts of IFN-γ will be produced. Mashhad University of Medical Sciences 2017-10 /pmc/articles/PMC5673696/ /pubmed/29147487 http://dx.doi.org/10.22038/IJBMS.2017.9445 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Teimourpour, Roghayeh
peeridogaheh, Hadi
Teimourpour, Amir
Arzanlou, Mohsen
Meshkat, Zahra
A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
title A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
title_full A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
title_fullStr A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
title_full_unstemmed A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
title_short A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
title_sort study on the immune response induced by a dna vaccine encoding mtb32c-hbha antigen of mycobacterium tuberculosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673696/
https://www.ncbi.nlm.nih.gov/pubmed/29147487
http://dx.doi.org/10.22038/IJBMS.2017.9445
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