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Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension
Hypertension is a systemic disorder that affects numerous physiological processes throughout the body. Improper sodium transport is a common comorbidity of hypertension, and sodium transport is also critical for maintaining the secretion of submandibular glands, whether the function of submandibular...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674029/ https://www.ncbi.nlm.nih.gov/pubmed/29109472 http://dx.doi.org/10.1038/s41598-017-15211-0 |
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author | Zhang, Jing Zhong, Li-Jun Wang, Yang Liu, Li-mei Cong, Xin Xiang, Ruo-Lan Wu, Li-Ling Yu, Guang-Yan Zhang, Yan |
author_facet | Zhang, Jing Zhong, Li-Jun Wang, Yang Liu, Li-mei Cong, Xin Xiang, Ruo-Lan Wu, Li-Ling Yu, Guang-Yan Zhang, Yan |
author_sort | Zhang, Jing |
collection | PubMed |
description | Hypertension is a systemic disorder that affects numerous physiological processes throughout the body. Improper sodium transport is a common comorbidity of hypertension, and sodium transport is also critical for maintaining the secretion of submandibular glands, whether the function of submandibular glands is affected by hypertension remains unclear. To determine whether hypertension induces changes in the protein expression of submandibular glands, we compared the proteome of submandibular glands from 14-week-old spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats using LC-MS/MS. The results revealed that 95 proteins displayed different levels of expression between the submandibular glands from the SHRs and WKYs. Among these, 35 proteins were more abundant, and 60 proteins were less abundant in the SHR compared with the WKY rats. Specifically, aquaporin 5 and parvalbumin, which are correlated with water transport and intracellular Ca(2+) signal transduction, were verified to exhibit differences in protein abundance. Impaired Ca(2+) response to carbachol was confirmed in the acinar cells from SHRs, and hyposecretion by the submandibular glands was further confirmed by in vivo saliva collection. In conclusion, the proteomic analysis of the submandibular glands of SHRs revealed novel changes in protein abundance that provides possible mechanisms connecting hypertension and hyposecretion in submandibular glands. |
format | Online Article Text |
id | pubmed-5674029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56740292017-11-15 Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension Zhang, Jing Zhong, Li-Jun Wang, Yang Liu, Li-mei Cong, Xin Xiang, Ruo-Lan Wu, Li-Ling Yu, Guang-Yan Zhang, Yan Sci Rep Article Hypertension is a systemic disorder that affects numerous physiological processes throughout the body. Improper sodium transport is a common comorbidity of hypertension, and sodium transport is also critical for maintaining the secretion of submandibular glands, whether the function of submandibular glands is affected by hypertension remains unclear. To determine whether hypertension induces changes in the protein expression of submandibular glands, we compared the proteome of submandibular glands from 14-week-old spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats using LC-MS/MS. The results revealed that 95 proteins displayed different levels of expression between the submandibular glands from the SHRs and WKYs. Among these, 35 proteins were more abundant, and 60 proteins were less abundant in the SHR compared with the WKY rats. Specifically, aquaporin 5 and parvalbumin, which are correlated with water transport and intracellular Ca(2+) signal transduction, were verified to exhibit differences in protein abundance. Impaired Ca(2+) response to carbachol was confirmed in the acinar cells from SHRs, and hyposecretion by the submandibular glands was further confirmed by in vivo saliva collection. In conclusion, the proteomic analysis of the submandibular glands of SHRs revealed novel changes in protein abundance that provides possible mechanisms connecting hypertension and hyposecretion in submandibular glands. Nature Publishing Group UK 2017-11-06 /pmc/articles/PMC5674029/ /pubmed/29109472 http://dx.doi.org/10.1038/s41598-017-15211-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhang, Jing Zhong, Li-Jun Wang, Yang Liu, Li-mei Cong, Xin Xiang, Ruo-Lan Wu, Li-Ling Yu, Guang-Yan Zhang, Yan Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension |
title | Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension |
title_full | Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension |
title_fullStr | Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension |
title_full_unstemmed | Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension |
title_short | Proteomic analysis reveals an impaired Ca(2+)/AQP5 pathway in the submandibular gland in hypertension |
title_sort | proteomic analysis reveals an impaired ca(2+)/aqp5 pathway in the submandibular gland in hypertension |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674029/ https://www.ncbi.nlm.nih.gov/pubmed/29109472 http://dx.doi.org/10.1038/s41598-017-15211-0 |
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