Improving Fab’ fragment retention in an autonucleolytic Escherichia coli strain by swapping periplasmic nuclease translocation signal from OmpA to DsbA

OBJECTIVES: To reduce unwanted Fab’ leakage from an autonucleolytic Escherichia coli strain, which co-expresses OmpA-signalled Staphylococcal nuclease and Fab’ fragment in the periplasm, by substituting in Serratial nuclease and the DsbA periplasm translocation signal as alternatives. RESULTS: We at...

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Detalles Bibliográficos
Autores principales: Schofield, Desmond M., Sirka, Ernestas, Keshavarz-Moore, Eli, Ward, John M., Nesbeth, Darren N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674116/
https://www.ncbi.nlm.nih.gov/pubmed/28875244
http://dx.doi.org/10.1007/s10529-017-2425-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674116/
https://www.ncbi.nlm.nih.gov/pubmed/28875244
http://dx.doi.org/10.1007/s10529-017-2425-z

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