Cargando…

A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry

AIM: Expression of PD-L1 in the tumor is associated with more favorable responses to anti-PD-1 therapy in multiple cancers. However, obtaining tumor biopsies for PD-L1 interrogation is an invasive procedure and challenging to assess repeatedly as the disease progresses. MATERIALS & METHODS: Here...

Descripción completa

Detalles Bibliográficos
Autores principales: Teo, Jinkai, Mirenska, Anja, Tan, Meihui, Lee, Yifang, Oh, Janice, Hong, Lewis Z, Wnek, Richard, Yap, Yoon-Sim, Shih, Shian-Jiun, S Bhagat, Ali Asgar, Chin, Chih-Liang, Skibinski, David AG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674273/
https://www.ncbi.nlm.nih.gov/pubmed/29134128
http://dx.doi.org/10.4155/fsoa-2017-0079
_version_ 1783276737797292032
author Teo, Jinkai
Mirenska, Anja
Tan, Meihui
Lee, Yifang
Oh, Janice
Hong, Lewis Z
Wnek, Richard
Yap, Yoon-Sim
Shih, Shian-Jiun
S Bhagat, Ali Asgar
Chin, Chih-Liang
Skibinski, David AG
author_facet Teo, Jinkai
Mirenska, Anja
Tan, Meihui
Lee, Yifang
Oh, Janice
Hong, Lewis Z
Wnek, Richard
Yap, Yoon-Sim
Shih, Shian-Jiun
S Bhagat, Ali Asgar
Chin, Chih-Liang
Skibinski, David AG
author_sort Teo, Jinkai
collection PubMed
description AIM: Expression of PD-L1 in the tumor is associated with more favorable responses to anti-PD-1 therapy in multiple cancers. However, obtaining tumor biopsies for PD-L1 interrogation is an invasive procedure and challenging to assess repeatedly as the disease progresses. MATERIALS & METHODS: Here we assess an alternative, minimally invasive approach to analyze blood samples for circulating tumor cells (CTCs) that have broken away from the tumor and entered the periphery. Our approach uses sized-based microfluidic CTC enrichment and subsequent characterization with microfluidic-based cytometry (chipcytometry). CONCLUSION: We demonstrate tumor-cell detection and characterization for PD-L1, and other markers, in both spiked and patient samples. This preliminary communication is the first report using chipcytometry for the characterization of CTCs.
format Online
Article
Text
id pubmed-5674273
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Future Science Ltd
record_format MEDLINE/PubMed
spelling pubmed-56742732017-11-13 A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry Teo, Jinkai Mirenska, Anja Tan, Meihui Lee, Yifang Oh, Janice Hong, Lewis Z Wnek, Richard Yap, Yoon-Sim Shih, Shian-Jiun S Bhagat, Ali Asgar Chin, Chih-Liang Skibinski, David AG Future Sci OA Preliminary Communication AIM: Expression of PD-L1 in the tumor is associated with more favorable responses to anti-PD-1 therapy in multiple cancers. However, obtaining tumor biopsies for PD-L1 interrogation is an invasive procedure and challenging to assess repeatedly as the disease progresses. MATERIALS & METHODS: Here we assess an alternative, minimally invasive approach to analyze blood samples for circulating tumor cells (CTCs) that have broken away from the tumor and entered the periphery. Our approach uses sized-based microfluidic CTC enrichment and subsequent characterization with microfluidic-based cytometry (chipcytometry). CONCLUSION: We demonstrate tumor-cell detection and characterization for PD-L1, and other markers, in both spiked and patient samples. This preliminary communication is the first report using chipcytometry for the characterization of CTCs. Future Science Ltd 2017-09-04 /pmc/articles/PMC5674273/ /pubmed/29134128 http://dx.doi.org/10.4155/fsoa-2017-0079 Text en © 2017 Teo et al. This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Preliminary Communication
Teo, Jinkai
Mirenska, Anja
Tan, Meihui
Lee, Yifang
Oh, Janice
Hong, Lewis Z
Wnek, Richard
Yap, Yoon-Sim
Shih, Shian-Jiun
S Bhagat, Ali Asgar
Chin, Chih-Liang
Skibinski, David AG
A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
title A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
title_full A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
title_fullStr A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
title_full_unstemmed A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
title_short A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
title_sort preliminary study for the assessment of pd-l1 and pd-l2 on circulating tumor cells by microfluidic-based chipcytometry
topic Preliminary Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674273/
https://www.ncbi.nlm.nih.gov/pubmed/29134128
http://dx.doi.org/10.4155/fsoa-2017-0079
work_keys_str_mv AT teojinkai apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT mirenskaanja apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT tanmeihui apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT leeyifang apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT ohjanice apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT honglewisz apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT wnekrichard apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT yapyoonsim apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT shihshianjiun apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT sbhagataliasgar apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT chinchihliang apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT skibinskidavidag apreliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT teojinkai preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT mirenskaanja preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT tanmeihui preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT leeyifang preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT ohjanice preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT honglewisz preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT wnekrichard preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT yapyoonsim preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT shihshianjiun preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT sbhagataliasgar preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT chinchihliang preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry
AT skibinskidavidag preliminarystudyfortheassessmentofpdl1andpdl2oncirculatingtumorcellsbymicrofluidicbasedchipcytometry