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A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry
AIM: Expression of PD-L1 in the tumor is associated with more favorable responses to anti-PD-1 therapy in multiple cancers. However, obtaining tumor biopsies for PD-L1 interrogation is an invasive procedure and challenging to assess repeatedly as the disease progresses. MATERIALS & METHODS: Here...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674273/ https://www.ncbi.nlm.nih.gov/pubmed/29134128 http://dx.doi.org/10.4155/fsoa-2017-0079 |
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author | Teo, Jinkai Mirenska, Anja Tan, Meihui Lee, Yifang Oh, Janice Hong, Lewis Z Wnek, Richard Yap, Yoon-Sim Shih, Shian-Jiun S Bhagat, Ali Asgar Chin, Chih-Liang Skibinski, David AG |
author_facet | Teo, Jinkai Mirenska, Anja Tan, Meihui Lee, Yifang Oh, Janice Hong, Lewis Z Wnek, Richard Yap, Yoon-Sim Shih, Shian-Jiun S Bhagat, Ali Asgar Chin, Chih-Liang Skibinski, David AG |
author_sort | Teo, Jinkai |
collection | PubMed |
description | AIM: Expression of PD-L1 in the tumor is associated with more favorable responses to anti-PD-1 therapy in multiple cancers. However, obtaining tumor biopsies for PD-L1 interrogation is an invasive procedure and challenging to assess repeatedly as the disease progresses. MATERIALS & METHODS: Here we assess an alternative, minimally invasive approach to analyze blood samples for circulating tumor cells (CTCs) that have broken away from the tumor and entered the periphery. Our approach uses sized-based microfluidic CTC enrichment and subsequent characterization with microfluidic-based cytometry (chipcytometry). CONCLUSION: We demonstrate tumor-cell detection and characterization for PD-L1, and other markers, in both spiked and patient samples. This preliminary communication is the first report using chipcytometry for the characterization of CTCs. |
format | Online Article Text |
id | pubmed-5674273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56742732017-11-13 A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry Teo, Jinkai Mirenska, Anja Tan, Meihui Lee, Yifang Oh, Janice Hong, Lewis Z Wnek, Richard Yap, Yoon-Sim Shih, Shian-Jiun S Bhagat, Ali Asgar Chin, Chih-Liang Skibinski, David AG Future Sci OA Preliminary Communication AIM: Expression of PD-L1 in the tumor is associated with more favorable responses to anti-PD-1 therapy in multiple cancers. However, obtaining tumor biopsies for PD-L1 interrogation is an invasive procedure and challenging to assess repeatedly as the disease progresses. MATERIALS & METHODS: Here we assess an alternative, minimally invasive approach to analyze blood samples for circulating tumor cells (CTCs) that have broken away from the tumor and entered the periphery. Our approach uses sized-based microfluidic CTC enrichment and subsequent characterization with microfluidic-based cytometry (chipcytometry). CONCLUSION: We demonstrate tumor-cell detection and characterization for PD-L1, and other markers, in both spiked and patient samples. This preliminary communication is the first report using chipcytometry for the characterization of CTCs. Future Science Ltd 2017-09-04 /pmc/articles/PMC5674273/ /pubmed/29134128 http://dx.doi.org/10.4155/fsoa-2017-0079 Text en © 2017 Teo et al. This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Preliminary Communication Teo, Jinkai Mirenska, Anja Tan, Meihui Lee, Yifang Oh, Janice Hong, Lewis Z Wnek, Richard Yap, Yoon-Sim Shih, Shian-Jiun S Bhagat, Ali Asgar Chin, Chih-Liang Skibinski, David AG A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry |
title | A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry |
title_full | A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry |
title_fullStr | A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry |
title_full_unstemmed | A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry |
title_short | A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry |
title_sort | preliminary study for the assessment of pd-l1 and pd-l2 on circulating tumor cells by microfluidic-based chipcytometry |
topic | Preliminary Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674273/ https://www.ncbi.nlm.nih.gov/pubmed/29134128 http://dx.doi.org/10.4155/fsoa-2017-0079 |
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