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Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae

AIM: Carbapenems are antibiotics reserved for treatment of severe infections. Carbapenem antimicrobial susceptibility testing profiles were determined in a population of Klebsiella pneumoniae, and their resistance assessed based on previous and current Clinical and Laboratory Standards Institute cri...

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Autores principales: Enyinnaya, Fidelis, Cruz, Patricia, Buttner, Mark P, Cross, Chad, Woodard, David R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674275/
https://www.ncbi.nlm.nih.gov/pubmed/29134129
http://dx.doi.org/10.4155/fsoa-2017-0095
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author Enyinnaya, Fidelis
Cruz, Patricia
Buttner, Mark P
Cross, Chad
Woodard, David R
author_facet Enyinnaya, Fidelis
Cruz, Patricia
Buttner, Mark P
Cross, Chad
Woodard, David R
author_sort Enyinnaya, Fidelis
collection PubMed
description AIM: Carbapenems are antibiotics reserved for treatment of severe infections. Carbapenem antimicrobial susceptibility testing profiles were determined in a population of Klebsiella pneumoniae, and their resistance assessed based on previous and current Clinical and Laboratory Standards Institute criteria. MATERIALS & METHODS: Isolates were examined using an automated antimicrobial susceptibility testing method, and real time polymerase chain reaction to detect the resistance (blaKPC) gene. RESULTS: The prevalence of blaKPC gene was 45/54 (83.3%). Five isolates that were susceptible under the previous criteria changed to nonsusceptible with the current standards. The overall difference in susceptibility between the standards was 8%. CONCLUSION: This study shows that the current Clinical and Laboratory Standards Institute criteria may not offer additional benefits in the fight against carbapenem-resistant Enterobacteriaceae.
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spelling pubmed-56742752017-11-13 Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae Enyinnaya, Fidelis Cruz, Patricia Buttner, Mark P Cross, Chad Woodard, David R Future Sci OA Research Article AIM: Carbapenems are antibiotics reserved for treatment of severe infections. Carbapenem antimicrobial susceptibility testing profiles were determined in a population of Klebsiella pneumoniae, and their resistance assessed based on previous and current Clinical and Laboratory Standards Institute criteria. MATERIALS & METHODS: Isolates were examined using an automated antimicrobial susceptibility testing method, and real time polymerase chain reaction to detect the resistance (blaKPC) gene. RESULTS: The prevalence of blaKPC gene was 45/54 (83.3%). Five isolates that were susceptible under the previous criteria changed to nonsusceptible with the current standards. The overall difference in susceptibility between the standards was 8%. CONCLUSION: This study shows that the current Clinical and Laboratory Standards Institute criteria may not offer additional benefits in the fight against carbapenem-resistant Enterobacteriaceae. Future Science Ltd 2017-10-05 /pmc/articles/PMC5674275/ /pubmed/29134129 http://dx.doi.org/10.4155/fsoa-2017-0095 Text en © 2017 Patricia Cruz This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Research Article
Enyinnaya, Fidelis
Cruz, Patricia
Buttner, Mark P
Cross, Chad
Woodard, David R
Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae
title Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae
title_full Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae
title_fullStr Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae
title_full_unstemmed Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae
title_short Comparison of Clinical & Laboratory Standards Institute standards in antimicrobial susceptibility among the carbapenemase producing Enterobacteriaceae
title_sort comparison of clinical & laboratory standards institute standards in antimicrobial susceptibility among the carbapenemase producing enterobacteriaceae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674275/
https://www.ncbi.nlm.nih.gov/pubmed/29134129
http://dx.doi.org/10.4155/fsoa-2017-0095
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