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Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice

OBJECTIVE: To determine whether inclusion of a refractory period between bouts of low magnitude mechanical stimulation (LMMS) can curb obesity-induced adipose tissue dysfunction and sequelae in adult mice. METHODS: A diet-induced obesity model with 45kcal% fat diet was employed with intention-to-tre...

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Autores principales: Patel, Vihitaben S., Chan, M. Ete, Pagnotti, Gabriel M., Frechette, Danielle M., Rubin, Janet, Rubin, Clinton T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675136/
https://www.ncbi.nlm.nih.gov/pubmed/28840647
http://dx.doi.org/10.1002/oby.21958
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author Patel, Vihitaben S.
Chan, M. Ete
Pagnotti, Gabriel M.
Frechette, Danielle M.
Rubin, Janet
Rubin, Clinton T.
author_facet Patel, Vihitaben S.
Chan, M. Ete
Pagnotti, Gabriel M.
Frechette, Danielle M.
Rubin, Janet
Rubin, Clinton T.
author_sort Patel, Vihitaben S.
collection PubMed
description OBJECTIVE: To determine whether inclusion of a refractory period between bouts of low magnitude mechanical stimulation (LMMS) can curb obesity-induced adipose tissue dysfunction and sequelae in adult mice. METHODS: A diet-induced obesity model with 45kcal% fat diet was employed with intention-to-treat. C57BL/6J mice were weight-matched into four groups: low fat diet (LFD, n=8), high fat diet (HF, n=8), high fat diet with one bout of 30min LMMS (HFv, n=9), and high fat diet with two bouts of 15min LMMS with 5-hour separation (refractory period, RHFv, n=9). 2-week of diet was followed by 6-week of diet+LMMS. RESULTS: HF and HFv continued gaining body weight and visceral adiposity throughout the experiment, which was mitigated in RHFv. HF and HFv had increased adipocyte hypertrophy, immune cell infiltration (B-cells, T cells, and macrophages) into adipose tissue, adipose tissue inflammation (TNF-α gene expression), and decreased proportion of mesenchymal stem cells in adipose tissue, as compared to LFD, all of which was rescued in RHFv. Glucose intolerance, sequelae of adipose tissue dysfunction, were elevated in HF and HFv, but not in RHFv, as compared to LFD. CONCLUSION: Incorporating a 5-hour refractory period between bouts of LMMS attenuates obesity-induced adipose tissue dysfunction and mitigates glucose intolerance.
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spelling pubmed-56751362018-02-25 Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice Patel, Vihitaben S. Chan, M. Ete Pagnotti, Gabriel M. Frechette, Danielle M. Rubin, Janet Rubin, Clinton T. Obesity (Silver Spring) Article OBJECTIVE: To determine whether inclusion of a refractory period between bouts of low magnitude mechanical stimulation (LMMS) can curb obesity-induced adipose tissue dysfunction and sequelae in adult mice. METHODS: A diet-induced obesity model with 45kcal% fat diet was employed with intention-to-treat. C57BL/6J mice were weight-matched into four groups: low fat diet (LFD, n=8), high fat diet (HF, n=8), high fat diet with one bout of 30min LMMS (HFv, n=9), and high fat diet with two bouts of 15min LMMS with 5-hour separation (refractory period, RHFv, n=9). 2-week of diet was followed by 6-week of diet+LMMS. RESULTS: HF and HFv continued gaining body weight and visceral adiposity throughout the experiment, which was mitigated in RHFv. HF and HFv had increased adipocyte hypertrophy, immune cell infiltration (B-cells, T cells, and macrophages) into adipose tissue, adipose tissue inflammation (TNF-α gene expression), and decreased proportion of mesenchymal stem cells in adipose tissue, as compared to LFD, all of which was rescued in RHFv. Glucose intolerance, sequelae of adipose tissue dysfunction, were elevated in HF and HFv, but not in RHFv, as compared to LFD. CONCLUSION: Incorporating a 5-hour refractory period between bouts of LMMS attenuates obesity-induced adipose tissue dysfunction and mitigates glucose intolerance. 2017-08-25 2017-10 /pmc/articles/PMC5675136/ /pubmed/28840647 http://dx.doi.org/10.1002/oby.21958 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Patel, Vihitaben S.
Chan, M. Ete
Pagnotti, Gabriel M.
Frechette, Danielle M.
Rubin, Janet
Rubin, Clinton T.
Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice
title Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice
title_full Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice
title_fullStr Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice
title_full_unstemmed Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice
title_short Incorporating Refractory Period in Mechanical Stimulation Mitigates Obesity-Induced Adipose Tissue Dysfunction in Adult Mice
title_sort incorporating refractory period in mechanical stimulation mitigates obesity-induced adipose tissue dysfunction in adult mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675136/
https://www.ncbi.nlm.nih.gov/pubmed/28840647
http://dx.doi.org/10.1002/oby.21958
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