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Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo

Formation of epiblast (EPI) – the founder line of all embryonic lineages – and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively on the mouse. Here, we provide a comprehensive, stage-b...

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Autores principales: Piliszek, Anna, Madeja, Zofia E., Plusa, Berenika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675450/
https://www.ncbi.nlm.nih.gov/pubmed/28935706
http://dx.doi.org/10.1242/dev.156406
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author Piliszek, Anna
Madeja, Zofia E.
Plusa, Berenika
author_facet Piliszek, Anna
Madeja, Zofia E.
Plusa, Berenika
author_sort Piliszek, Anna
collection PubMed
description Formation of epiblast (EPI) – the founder line of all embryonic lineages – and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively on the mouse. Here, we provide a comprehensive, stage-by-stage analysis of EPI and extra-embryonic primitive endoderm (PrE) formation during preimplantation development of the rabbit. Although we observed that rabbit embryos have several features in common with mouse embryos, including a stage-related initiation of lineage specification, our results demonstrate the existence of some key differences in lineage specification among mammals. Contrary to the current view, our data suggest that reciprocal repression of GATA6 and NANOG is not fundamental for the initial stages of PrE versus EPI specification in mammals. Furthermore, our results provide insight into the observed discrepancies relating to the role of FGF/ERK signalling in PrE versus EPI specification between mouse and other mammals.
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spelling pubmed-56754502017-11-20 Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo Piliszek, Anna Madeja, Zofia E. Plusa, Berenika Development Research Article Formation of epiblast (EPI) – the founder line of all embryonic lineages – and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively on the mouse. Here, we provide a comprehensive, stage-by-stage analysis of EPI and extra-embryonic primitive endoderm (PrE) formation during preimplantation development of the rabbit. Although we observed that rabbit embryos have several features in common with mouse embryos, including a stage-related initiation of lineage specification, our results demonstrate the existence of some key differences in lineage specification among mammals. Contrary to the current view, our data suggest that reciprocal repression of GATA6 and NANOG is not fundamental for the initial stages of PrE versus EPI specification in mammals. Furthermore, our results provide insight into the observed discrepancies relating to the role of FGF/ERK signalling in PrE versus EPI specification between mouse and other mammals. The Company of Biologists Ltd 2017-10-15 /pmc/articles/PMC5675450/ /pubmed/28935706 http://dx.doi.org/10.1242/dev.156406 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Piliszek, Anna
Madeja, Zofia E.
Plusa, Berenika
Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
title Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
title_full Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
title_fullStr Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
title_full_unstemmed Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
title_short Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
title_sort suppression of erk signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675450/
https://www.ncbi.nlm.nih.gov/pubmed/28935706
http://dx.doi.org/10.1242/dev.156406
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