A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)

Abstract. Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the presen...

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Autores principales: Schmidt, Olga, Hausmann, Axel, Cancian de Araujo, Bruno, Sutrisno, Hari, Peggie, Djunijanti, Schmidt, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676197/
https://www.ncbi.nlm.nih.gov/pubmed/29134041
http://dx.doi.org/10.3897/BDJ.5.e20006
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author Schmidt, Olga
Hausmann, Axel
Cancian de Araujo, Bruno
Sutrisno, Hari
Peggie, Djunijanti
Schmidt, Stefan
author_facet Schmidt, Olga
Hausmann, Axel
Cancian de Araujo, Bruno
Sutrisno, Hari
Peggie, Djunijanti
Schmidt, Stefan
author_sort Schmidt, Olga
collection PubMed
description Abstract. Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the Museum für Naturkunde in Berlin and the Zoologische Staatssammlung München, in close cooperation with the Research Center for Biology – Indonesian Institute of Sciences (RCB-LIPI, Bogor).
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spelling pubmed-56761972017-11-13 A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys) Schmidt, Olga Hausmann, Axel Cancian de Araujo, Bruno Sutrisno, Hari Peggie, Djunijanti Schmidt, Stefan Biodivers Data J Research Article Abstract. Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the Museum für Naturkunde in Berlin and the Zoologische Staatssammlung München, in close cooperation with the Research Center for Biology – Indonesian Institute of Sciences (RCB-LIPI, Bogor). Pensoft Publishers 2017-10-31 /pmc/articles/PMC5676197/ /pubmed/29134041 http://dx.doi.org/10.3897/BDJ.5.e20006 Text en Olga Schmidt, Axel Hausmann, Bruno Cancian de Araujo, Hari Sutrisno, Djunijanti Peggie, Stefan Schmidt http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Schmidt, Olga
Hausmann, Axel
Cancian de Araujo, Bruno
Sutrisno, Hari
Peggie, Djunijanti
Schmidt, Stefan
A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
title A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
title_full A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
title_fullStr A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
title_full_unstemmed A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
title_short A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)
title_sort streamlined collecting and preparation protocol for dna barcoding of lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the indonesian biodiversity discovery and information system (indobiosys)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676197/
https://www.ncbi.nlm.nih.gov/pubmed/29134041
http://dx.doi.org/10.3897/BDJ.5.e20006
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