Cargando…
Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.)
Seed number is one of the key traits related to plant evolution/domestication and crop improvement/breeding. In rapeseed germplasm, the seed number per pod (SNPP) shows a very wide variation from several to nearly 30; however, the underlying causations/mechanisms for this variation are poorly known....
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676210/ https://www.ncbi.nlm.nih.gov/pubmed/29163611 http://dx.doi.org/10.3389/fpls.2017.01890 |
_version_ | 1783277025280131072 |
---|---|
author | Yang, Yuhua Wang, Ying Zhan, Jiepeng Shi, Jiaqin Wang, Xinfa Liu, Guihua Wang, Hanzhong |
author_facet | Yang, Yuhua Wang, Ying Zhan, Jiepeng Shi, Jiaqin Wang, Xinfa Liu, Guihua Wang, Hanzhong |
author_sort | Yang, Yuhua |
collection | PubMed |
description | Seed number is one of the key traits related to plant evolution/domestication and crop improvement/breeding. In rapeseed germplasm, the seed number per pod (SNPP) shows a very wide variation from several to nearly 30; however, the underlying causations/mechanisms for this variation are poorly known. In the current study, the genetic and cytological bases for the natural variation of SNPP in rapeseed was firstly and systematically investigated using the representative four high-SNPP and five low-SNPP lines. The results of self- or cross-pollination experiment between the high- and low-SNPP lines showed that the natural variation of SNPP was mainly controlled by maternal effect (mean = 0.79), followed by paternal effect (mean = 0.21). Analysis of the data using diploid seed embryo–cytoplasmic–maternal model further showed that the maternal genotype, embryo, and cytoplasm effects, respectively, explained 47.6, 35.2, and 7.5% of the genetic variance. In addition, the analysis of combining ability showed that for the SNPP of hybrid F(1) was mainly determined by the general combining ability of parents (63.0%), followed by special combining ability of parental combination (37.0%). More importantly, the cytological observation showed that the SNPP difference between the high- and low-SNPP lines was attributable to the accumulative differences in its components. Of which, the number of ovules, the proportion of fertile ovules, the proportion of fertile ovules to be fertilized, and the proportion of fertilized ovules to develop into seeds accounted for 30.7, 18.2, 7.1, and 43.9%, respectively. The accordant results of both genetic and cytological analyses provide solid evidences and systematic insights to further understand the mechanisms underlying the natural variation of SNPP, which will facilitate the development of high-yield cultivars in rapeseed. |
format | Online Article Text |
id | pubmed-5676210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56762102017-11-21 Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) Yang, Yuhua Wang, Ying Zhan, Jiepeng Shi, Jiaqin Wang, Xinfa Liu, Guihua Wang, Hanzhong Front Plant Sci Plant Science Seed number is one of the key traits related to plant evolution/domestication and crop improvement/breeding. In rapeseed germplasm, the seed number per pod (SNPP) shows a very wide variation from several to nearly 30; however, the underlying causations/mechanisms for this variation are poorly known. In the current study, the genetic and cytological bases for the natural variation of SNPP in rapeseed was firstly and systematically investigated using the representative four high-SNPP and five low-SNPP lines. The results of self- or cross-pollination experiment between the high- and low-SNPP lines showed that the natural variation of SNPP was mainly controlled by maternal effect (mean = 0.79), followed by paternal effect (mean = 0.21). Analysis of the data using diploid seed embryo–cytoplasmic–maternal model further showed that the maternal genotype, embryo, and cytoplasm effects, respectively, explained 47.6, 35.2, and 7.5% of the genetic variance. In addition, the analysis of combining ability showed that for the SNPP of hybrid F(1) was mainly determined by the general combining ability of parents (63.0%), followed by special combining ability of parental combination (37.0%). More importantly, the cytological observation showed that the SNPP difference between the high- and low-SNPP lines was attributable to the accumulative differences in its components. Of which, the number of ovules, the proportion of fertile ovules, the proportion of fertile ovules to be fertilized, and the proportion of fertilized ovules to develop into seeds accounted for 30.7, 18.2, 7.1, and 43.9%, respectively. The accordant results of both genetic and cytological analyses provide solid evidences and systematic insights to further understand the mechanisms underlying the natural variation of SNPP, which will facilitate the development of high-yield cultivars in rapeseed. Frontiers Media S.A. 2017-11-02 /pmc/articles/PMC5676210/ /pubmed/29163611 http://dx.doi.org/10.3389/fpls.2017.01890 Text en Copyright © 2017 Yang, Wang, Zhan, Shi, Wang, Liu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yang, Yuhua Wang, Ying Zhan, Jiepeng Shi, Jiaqin Wang, Xinfa Liu, Guihua Wang, Hanzhong Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) |
title | Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) |
title_full | Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) |
title_fullStr | Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) |
title_full_unstemmed | Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) |
title_short | Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.) |
title_sort | genetic and cytological analyses of the natural variation of seed number per pod in rapeseed (brassica napus l.) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676210/ https://www.ncbi.nlm.nih.gov/pubmed/29163611 http://dx.doi.org/10.3389/fpls.2017.01890 |
work_keys_str_mv | AT yangyuhua geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl AT wangying geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl AT zhanjiepeng geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl AT shijiaqin geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl AT wangxinfa geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl AT liuguihua geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl AT wanghanzhong geneticandcytologicalanalysesofthenaturalvariationofseednumberperpodinrapeseedbrassicanapusl |