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AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility
AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work's aims are AMPK identification, intracellular localization, and their role...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676432/ https://www.ncbi.nlm.nih.gov/pubmed/27678462 http://dx.doi.org/10.4103/1008-682X.185848 |
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author | Calle-Guisado, Violeta de Llera, Ana Hurtado Martin-Hidalgo, David Mijares, Jose Gil, Maria C Alvarez, Ignacio S Bragado, Maria J Garcia-Marin, Luis J |
author_facet | Calle-Guisado, Violeta de Llera, Ana Hurtado Martin-Hidalgo, David Mijares, Jose Gil, Maria C Alvarez, Ignacio S Bragado, Maria J Garcia-Marin, Luis J |
author_sort | Calle-Guisado, Violeta |
collection | PubMed |
description | AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work's aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Thr172-AMPK were analyzed by Western blotting and indirect immunofluorescence. High- and low-quality sperm populations were separated by a 40%–80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System (ISAS) in the presence or absence of the AMPK inhibitor compound C (CC). AMPK is localized along the human spermatozoa, at the entire acrosome, midpiece and tail with variable intensity, whereas its active form, phospho-Thr172-AMPK, shows a prominent staining at the acrosome and sperm tail with a weaker staining in the midpiece and the postacrosomal region. Interestingly, spermatozoa bearing an excess residual cytoplasm show strong AMPK staining in this subcellular compartment. Both AMPK and phospho-Thr172-AMPK human spermatozoa contents exhibit important individual variations. Moreover, active AMPK is predominant in the high motility sperm population, where shows a stronger intensity compared with the low motility sperm population. Inhibition of AMPK activity in human spermatozoa by CC treatment leads to a significant reduction in any sperm motility parameter analyzed: percent of motile sperm, sperm velocities, progressivity, and other motility coefficients. This work identifies and points out AMPK as a new molecular mechanism involved in human spermatozoa motility. Further AMPK implications in the clinical efficiency of assisted reproduction and in other reproductive areas need to be studied. |
format | Online Article Text |
id | pubmed-5676432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56764322017-11-17 AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility Calle-Guisado, Violeta de Llera, Ana Hurtado Martin-Hidalgo, David Mijares, Jose Gil, Maria C Alvarez, Ignacio S Bragado, Maria J Garcia-Marin, Luis J Asian J Androl Original Article AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work's aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Thr172-AMPK were analyzed by Western blotting and indirect immunofluorescence. High- and low-quality sperm populations were separated by a 40%–80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System (ISAS) in the presence or absence of the AMPK inhibitor compound C (CC). AMPK is localized along the human spermatozoa, at the entire acrosome, midpiece and tail with variable intensity, whereas its active form, phospho-Thr172-AMPK, shows a prominent staining at the acrosome and sperm tail with a weaker staining in the midpiece and the postacrosomal region. Interestingly, spermatozoa bearing an excess residual cytoplasm show strong AMPK staining in this subcellular compartment. Both AMPK and phospho-Thr172-AMPK human spermatozoa contents exhibit important individual variations. Moreover, active AMPK is predominant in the high motility sperm population, where shows a stronger intensity compared with the low motility sperm population. Inhibition of AMPK activity in human spermatozoa by CC treatment leads to a significant reduction in any sperm motility parameter analyzed: percent of motile sperm, sperm velocities, progressivity, and other motility coefficients. This work identifies and points out AMPK as a new molecular mechanism involved in human spermatozoa motility. Further AMPK implications in the clinical efficiency of assisted reproduction and in other reproductive areas need to be studied. Medknow Publications & Media Pvt Ltd 2017 2016-09-27 /pmc/articles/PMC5676432/ /pubmed/27678462 http://dx.doi.org/10.4103/1008-682X.185848 Text en Copyright: © The Author(s)(2017) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Calle-Guisado, Violeta de Llera, Ana Hurtado Martin-Hidalgo, David Mijares, Jose Gil, Maria C Alvarez, Ignacio S Bragado, Maria J Garcia-Marin, Luis J AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
title | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
title_full | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
title_fullStr | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
title_full_unstemmed | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
title_short | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
title_sort | amp-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676432/ https://www.ncbi.nlm.nih.gov/pubmed/27678462 http://dx.doi.org/10.4103/1008-682X.185848 |
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