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Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples
This study aims at providing recommendations concerning the validation of analytical protocols by using routine samples. It is intended to provide a case-study on how to validate the analytical methods in different environmental matrices. In order to analyze the selected compounds (pesticides and po...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676487/ https://www.ncbi.nlm.nih.gov/pubmed/29209555 http://dx.doi.org/10.1155/2017/9796457 |
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author | Pindado Jiménez, Oscar García Alonso, Susana Pérez Pastor, Rosa María |
author_facet | Pindado Jiménez, Oscar García Alonso, Susana Pérez Pastor, Rosa María |
author_sort | Pindado Jiménez, Oscar |
collection | PubMed |
description | This study aims at providing recommendations concerning the validation of analytical protocols by using routine samples. It is intended to provide a case-study on how to validate the analytical methods in different environmental matrices. In order to analyze the selected compounds (pesticides and polychlorinated biphenyls) in two different environmental matrices, the current work has performed and validated two analytical procedures by GC-MS. A description is given of the validation of the two protocols by the analysis of more than 30 samples of water and sediments collected along nine months. The present work also scopes the uncertainty associated with both analytical protocols. In detail, uncertainty of water sample was performed through a conventional approach. However, for the sediments matrices, the estimation of proportional/constant bias is also included due to its inhomogeneity. Results for the sediment matrix are reliable, showing a range 25–35% of analytical variability associated with intermediate conditions. The analytical methodology for the water matrix determines the selected compounds with acceptable recoveries and the combined uncertainty ranges between 20 and 30%. Analyzing routine samples is rarely applied to assess trueness of novel analytical methods and up to now this methodology was not focused on organochlorine compounds in environmental matrices. |
format | Online Article Text |
id | pubmed-5676487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-56764872017-12-05 Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples Pindado Jiménez, Oscar García Alonso, Susana Pérez Pastor, Rosa María J Anal Methods Chem Research Article This study aims at providing recommendations concerning the validation of analytical protocols by using routine samples. It is intended to provide a case-study on how to validate the analytical methods in different environmental matrices. In order to analyze the selected compounds (pesticides and polychlorinated biphenyls) in two different environmental matrices, the current work has performed and validated two analytical procedures by GC-MS. A description is given of the validation of the two protocols by the analysis of more than 30 samples of water and sediments collected along nine months. The present work also scopes the uncertainty associated with both analytical protocols. In detail, uncertainty of water sample was performed through a conventional approach. However, for the sediments matrices, the estimation of proportional/constant bias is also included due to its inhomogeneity. Results for the sediment matrix are reliable, showing a range 25–35% of analytical variability associated with intermediate conditions. The analytical methodology for the water matrix determines the selected compounds with acceptable recoveries and the combined uncertainty ranges between 20 and 30%. Analyzing routine samples is rarely applied to assess trueness of novel analytical methods and up to now this methodology was not focused on organochlorine compounds in environmental matrices. Hindawi 2017 2017-10-29 /pmc/articles/PMC5676487/ /pubmed/29209555 http://dx.doi.org/10.1155/2017/9796457 Text en Copyright © 2017 Oscar Pindado Jiménez et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pindado Jiménez, Oscar García Alonso, Susana Pérez Pastor, Rosa María Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
title | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
title_full | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
title_fullStr | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
title_full_unstemmed | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
title_short | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
title_sort | validating analytical protocols to determine selected pesticides and pcbs using routine samples |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676487/ https://www.ncbi.nlm.nih.gov/pubmed/29209555 http://dx.doi.org/10.1155/2017/9796457 |
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