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Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal
We present protein-PAINT – the implementation of the general principles of PAINT (Point Accumulation for Imaging in Nanoscale Topography) for live-cell protein labeling. Our method employs the specific binding of cell-permeable fluorogenic dyes to genetically encoded protein tags. We engineered thre...
Autores principales: | Bozhanova, Nina G., Baranov, Mikhail S., Klementieva, Natalia V., Sarkisyan, Karen S., Gavrikov, Alexey S., Yampolsky, Ilia V., Zagaynova, Elena V., Lukyanov, Sergey A., Lukyanov, Konstantin A., Mishin, Alexander S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676496/ https://www.ncbi.nlm.nih.gov/pubmed/29147545 http://dx.doi.org/10.1039/c7sc01628j |
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