Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis

Hexahistidines are very common tags used in the affinity chromatography purification of recombinant proteins. Although these tags are solely applied for their metal-binding properties, we found that they are also able to perform ester hydrolysis when attached to a protein. For instance, green fluore...

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Detalles Bibliográficos
Autores principales: Schoonen, Lise, van Esterik, Kayleigh S., Zhang, Chunqiu, Ulijn, Rein V., Nolte, Roeland J. M., Hest, Jan C. M. van
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676709/
https://www.ncbi.nlm.nih.gov/pubmed/29116178
http://dx.doi.org/10.1038/s41598-017-15310-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676709/
https://www.ncbi.nlm.nih.gov/pubmed/29116178
http://dx.doi.org/10.1038/s41598-017-15310-y

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