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Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma

The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) p...

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Autores principales: Uotani, Koji, Fujiwara, Tomohiro, Yoshida, Aki, Iwata, Shintaro, Morita, Takuya, Kiyono, Masahiro, Yokoo, Suguru, Kunisada, Toshiyuki, Takeda, Ken, Hasei, Joe, Numoto, Kunihiko, Nezu, Yutaka, Yonemoto, Tsukasa, Ishii, Takeshi, Kawai, Akira, Ochiya, Takahiro, Ozaki, Toshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676745/
https://www.ncbi.nlm.nih.gov/pubmed/29116117
http://dx.doi.org/10.1038/s41598-017-12660-5
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author Uotani, Koji
Fujiwara, Tomohiro
Yoshida, Aki
Iwata, Shintaro
Morita, Takuya
Kiyono, Masahiro
Yokoo, Suguru
Kunisada, Toshiyuki
Takeda, Ken
Hasei, Joe
Numoto, Kunihiko
Nezu, Yutaka
Yonemoto, Tsukasa
Ishii, Takeshi
Kawai, Akira
Ochiya, Takahiro
Ozaki, Toshifumi
author_facet Uotani, Koji
Fujiwara, Tomohiro
Yoshida, Aki
Iwata, Shintaro
Morita, Takuya
Kiyono, Masahiro
Yokoo, Suguru
Kunisada, Toshiyuki
Takeda, Ken
Hasei, Joe
Numoto, Kunihiko
Nezu, Yutaka
Yonemoto, Tsukasa
Ishii, Takeshi
Kawai, Akira
Ochiya, Takahiro
Ozaki, Toshifumi
author_sort Uotani, Koji
collection PubMed
description The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) patients remains unknown. Initial global miRNA screening using SS patient serum and SS cell culture media identified a signature of four upregulated miRNAs. Among these candidates, miR-92b-3p secretion from SS cells was confirmed, which was embedded within tumour-derived exosomes rather than argonaute-2. Animal experiments revealed a close correlation between serum miR-92b-3p levels and tumour dynamics. Clinical relevance was validated in two independent clinical cohorts, and we subsequently identified that serum miR-92b-3p levels were significantly higher in SS patients in comparison to that in healthy individuals. Moreover, serum miR-92b-3p was robust in discriminating patients with SS from the other STS patients and reflected tumour burden in SS patients. Overall, liquid biopsy using serum miR-92b-3p expression levels may represent a novel approach for monitoring tumour dynamics of SS.
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spelling pubmed-56767452017-11-15 Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma Uotani, Koji Fujiwara, Tomohiro Yoshida, Aki Iwata, Shintaro Morita, Takuya Kiyono, Masahiro Yokoo, Suguru Kunisada, Toshiyuki Takeda, Ken Hasei, Joe Numoto, Kunihiko Nezu, Yutaka Yonemoto, Tsukasa Ishii, Takeshi Kawai, Akira Ochiya, Takahiro Ozaki, Toshifumi Sci Rep Article The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) patients remains unknown. Initial global miRNA screening using SS patient serum and SS cell culture media identified a signature of four upregulated miRNAs. Among these candidates, miR-92b-3p secretion from SS cells was confirmed, which was embedded within tumour-derived exosomes rather than argonaute-2. Animal experiments revealed a close correlation between serum miR-92b-3p levels and tumour dynamics. Clinical relevance was validated in two independent clinical cohorts, and we subsequently identified that serum miR-92b-3p levels were significantly higher in SS patients in comparison to that in healthy individuals. Moreover, serum miR-92b-3p was robust in discriminating patients with SS from the other STS patients and reflected tumour burden in SS patients. Overall, liquid biopsy using serum miR-92b-3p expression levels may represent a novel approach for monitoring tumour dynamics of SS. Nature Publishing Group UK 2017-11-07 /pmc/articles/PMC5676745/ /pubmed/29116117 http://dx.doi.org/10.1038/s41598-017-12660-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Uotani, Koji
Fujiwara, Tomohiro
Yoshida, Aki
Iwata, Shintaro
Morita, Takuya
Kiyono, Masahiro
Yokoo, Suguru
Kunisada, Toshiyuki
Takeda, Ken
Hasei, Joe
Numoto, Kunihiko
Nezu, Yutaka
Yonemoto, Tsukasa
Ishii, Takeshi
Kawai, Akira
Ochiya, Takahiro
Ozaki, Toshifumi
Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma
title Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma
title_full Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma
title_fullStr Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma
title_full_unstemmed Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma
title_short Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma
title_sort circulating microrna-92b-3p as a novel biomarker for monitoring of synovial sarcoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676745/
https://www.ncbi.nlm.nih.gov/pubmed/29116117
http://dx.doi.org/10.1038/s41598-017-12660-5
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