A Comparison of mRNA Sequencing with Random Primed and 3′-Directed Libraries

Creating a cDNA library for deep mRNA sequencing (mRNAseq) is generally done by random priming, creating multiple sequencing fragments along each transcript. A 3′-end-focused library approach cannot detect differential splicing, but has potentially higher throughput at a lower cost, along with the a...

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Detalles Bibliográficos
Autores principales: Xiong, Yuguang, Soumillon, Magali, Wu, Jie, Hansen, Jens, Hu, Bin, van Hasselt, Johan G. C., Jayaraman, Gomathi, Lim, Ryan, Bouhaddou, Mehdi, Ornelas, Loren, Bochicchio, Jim, Lenaeus, Lindsay, Stocksdale, Jennifer, Shim, Jaehee, Gomez, Emilda, Sareen, Dhruv, Svendsen, Clive, Thompson, Leslie M., Mahajan, Milind, Iyengar, Ravi, Sobie, Eric A., Azeloglu, Evren U., Birtwistle, Marc R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5676863/
https://www.ncbi.nlm.nih.gov/pubmed/29116112
http://dx.doi.org/10.1038/s41598-017-14892-x

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