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Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis

Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating ro...

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Detalles Bibliográficos
Autores principales: Kirschneck, Christian, Batschkus, Sarah, Proff, Peter, Köstler, Josef, Spanier, Gerrit, Schröder, Agnes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677027/
https://www.ncbi.nlm.nih.gov/pubmed/29116140
http://dx.doi.org/10.1038/s41598-017-15281-0