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First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp wit...

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Detalles Bibliográficos
Autores principales: Luo, Ruibang, Zimin, Aleksey, Workman, Rachael, Fan, Yunfan, Pertea, Geo, Grossman, Nina, Wear, Maggie P., Jia, Bei, Miller, Heather, Casadevall, Arturo, Timp, Winston, Zhang, Sean X., Salzberg, Steven L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677167/
https://www.ncbi.nlm.nih.gov/pubmed/28963165
http://dx.doi.org/10.1534/g3.117.300107
Descripción
Sumario:Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.