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First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp wit...

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Detalles Bibliográficos
Autores principales: Luo, Ruibang, Zimin, Aleksey, Workman, Rachael, Fan, Yunfan, Pertea, Geo, Grossman, Nina, Wear, Maggie P., Jia, Bei, Miller, Heather, Casadevall, Arturo, Timp, Winston, Zhang, Sean X., Salzberg, Steven L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677167/
https://www.ncbi.nlm.nih.gov/pubmed/28963165
http://dx.doi.org/10.1534/g3.117.300107
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author Luo, Ruibang
Zimin, Aleksey
Workman, Rachael
Fan, Yunfan
Pertea, Geo
Grossman, Nina
Wear, Maggie P.
Jia, Bei
Miller, Heather
Casadevall, Arturo
Timp, Winston
Zhang, Sean X.
Salzberg, Steven L.
author_facet Luo, Ruibang
Zimin, Aleksey
Workman, Rachael
Fan, Yunfan
Pertea, Geo
Grossman, Nina
Wear, Maggie P.
Jia, Bei
Miller, Heather
Casadevall, Arturo
Timp, Winston
Zhang, Sean X.
Salzberg, Steven L.
author_sort Luo, Ruibang
collection PubMed
description Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.
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spelling pubmed-56771672017-11-09 First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) Luo, Ruibang Zimin, Aleksey Workman, Rachael Fan, Yunfan Pertea, Geo Grossman, Nina Wear, Maggie P. Jia, Bei Miller, Heather Casadevall, Arturo Timp, Winston Zhang, Sean X. Salzberg, Steven L. G3 (Bethesda) Investigations Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb. Genetics Society of America 2017-09-29 /pmc/articles/PMC5677167/ /pubmed/28963165 http://dx.doi.org/10.1534/g3.117.300107 Text en Copyright © 2017 Luo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Luo, Ruibang
Zimin, Aleksey
Workman, Rachael
Fan, Yunfan
Pertea, Geo
Grossman, Nina
Wear, Maggie P.
Jia, Bei
Miller, Heather
Casadevall, Arturo
Timp, Winston
Zhang, Sean X.
Salzberg, Steven L.
First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
title First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
title_full First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
title_fullStr First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
title_full_unstemmed First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
title_short First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
title_sort first draft genome sequence of the pathogenic fungus lomentospora prolificans (formerly scedosporium prolificans)
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677167/
https://www.ncbi.nlm.nih.gov/pubmed/28963165
http://dx.doi.org/10.1534/g3.117.300107
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