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First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)
Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp wit...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677167/ https://www.ncbi.nlm.nih.gov/pubmed/28963165 http://dx.doi.org/10.1534/g3.117.300107 |
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author | Luo, Ruibang Zimin, Aleksey Workman, Rachael Fan, Yunfan Pertea, Geo Grossman, Nina Wear, Maggie P. Jia, Bei Miller, Heather Casadevall, Arturo Timp, Winston Zhang, Sean X. Salzberg, Steven L. |
author_facet | Luo, Ruibang Zimin, Aleksey Workman, Rachael Fan, Yunfan Pertea, Geo Grossman, Nina Wear, Maggie P. Jia, Bei Miller, Heather Casadevall, Arturo Timp, Winston Zhang, Sean X. Salzberg, Steven L. |
author_sort | Luo, Ruibang |
collection | PubMed |
description | Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb. |
format | Online Article Text |
id | pubmed-5677167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-56771672017-11-09 First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) Luo, Ruibang Zimin, Aleksey Workman, Rachael Fan, Yunfan Pertea, Geo Grossman, Nina Wear, Maggie P. Jia, Bei Miller, Heather Casadevall, Arturo Timp, Winston Zhang, Sean X. Salzberg, Steven L. G3 (Bethesda) Investigations Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb. Genetics Society of America 2017-09-29 /pmc/articles/PMC5677167/ /pubmed/28963165 http://dx.doi.org/10.1534/g3.117.300107 Text en Copyright © 2017 Luo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Luo, Ruibang Zimin, Aleksey Workman, Rachael Fan, Yunfan Pertea, Geo Grossman, Nina Wear, Maggie P. Jia, Bei Miller, Heather Casadevall, Arturo Timp, Winston Zhang, Sean X. Salzberg, Steven L. First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) |
title | First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) |
title_full | First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) |
title_fullStr | First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) |
title_full_unstemmed | First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) |
title_short | First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans) |
title_sort | first draft genome sequence of the pathogenic fungus lomentospora prolificans (formerly scedosporium prolificans) |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677167/ https://www.ncbi.nlm.nih.gov/pubmed/28963165 http://dx.doi.org/10.1534/g3.117.300107 |
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