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Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up
CONTEXT: Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. OBJECTIVE: To identify thyroid tumor-associated microRNAs (miRNAs) in the serum with potential for development as unique...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Endocrine Society
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677215/ https://www.ncbi.nlm.nih.gov/pubmed/29264441 http://dx.doi.org/10.1210/js.2016-1032 |
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author | Rosignolo, Francesca Sponziello, Marialuisa Giacomelli, Laura Russo, Diego Pecce, Valeria Biffoni, Marco Bellantone, Rocco Lombardi, Celestino Pio Lamartina, Livia Grani, Giorgio Durante, Cosimo Filetti, Sebastiano Verrienti, Antonella |
author_facet | Rosignolo, Francesca Sponziello, Marialuisa Giacomelli, Laura Russo, Diego Pecce, Valeria Biffoni, Marco Bellantone, Rocco Lombardi, Celestino Pio Lamartina, Livia Grani, Giorgio Durante, Cosimo Filetti, Sebastiano Verrienti, Antonella |
author_sort | Rosignolo, Francesca |
collection | PubMed |
description | CONTEXT: Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. OBJECTIVE: To identify thyroid tumor-associated microRNAs (miRNAs) in the serum with potential for development as unique biomarkers of PTC recurrence. METHODS: We measured expression of 754 miRNAs in serum samples collected from 11 patients with PTC before and 30 days after thyroidectomy. Major candidates were then re-evaluated by absolute quantitative polymerase chain reaction analysis in an independent cohort of patients with PTC (n = 44) or benign nodules and 20 healthy controls (HCs). The 2 miRNAs most significantly associated with thyroid tumors were then assessed in matched serum samples (before and 30 days and 1 to 2 years after surgery) from the 20 PTC patients with complete follow-up datasets and results correlated with American Thyroid Association (ATA) responses to therapy. RESULTS: Eight miRNAs (miR-221-3p, miR-222-3p, miR-146a-5p, miR-24-3p, miR-146b-5p, miR-191-5p, miR-103a-3p, and miR-28-3p) displayed levels in prethyroidectomy serum samples from patients with PTC that significantly exceeded those measured after thyroidectomy and those found in samples from HCs. The 2 most promising candidates—miR-146a-5p and miR-221-3p —were further analyzed in the 20 PTC patients mentioned earlier. Serum levels of both miRNAs after 1 to 2 years of follow-up were consistent with ATA responses to therapy in all patients, including 2 with structural evidence of disease whose Tg assays remained negative (<1 ng/mL). CONCLUSION: miR-146a-5p and miR-221-3p hold remarkable promise as serum biomarkers for post-treatment monitoring of PTC patients, especially when Tg assay results are uninformative. |
format | Online Article Text |
id | pubmed-5677215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Endocrine Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-56772152017-12-20 Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up Rosignolo, Francesca Sponziello, Marialuisa Giacomelli, Laura Russo, Diego Pecce, Valeria Biffoni, Marco Bellantone, Rocco Lombardi, Celestino Pio Lamartina, Livia Grani, Giorgio Durante, Cosimo Filetti, Sebastiano Verrienti, Antonella J Endocr Soc Research Article CONTEXT: Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. OBJECTIVE: To identify thyroid tumor-associated microRNAs (miRNAs) in the serum with potential for development as unique biomarkers of PTC recurrence. METHODS: We measured expression of 754 miRNAs in serum samples collected from 11 patients with PTC before and 30 days after thyroidectomy. Major candidates were then re-evaluated by absolute quantitative polymerase chain reaction analysis in an independent cohort of patients with PTC (n = 44) or benign nodules and 20 healthy controls (HCs). The 2 miRNAs most significantly associated with thyroid tumors were then assessed in matched serum samples (before and 30 days and 1 to 2 years after surgery) from the 20 PTC patients with complete follow-up datasets and results correlated with American Thyroid Association (ATA) responses to therapy. RESULTS: Eight miRNAs (miR-221-3p, miR-222-3p, miR-146a-5p, miR-24-3p, miR-146b-5p, miR-191-5p, miR-103a-3p, and miR-28-3p) displayed levels in prethyroidectomy serum samples from patients with PTC that significantly exceeded those measured after thyroidectomy and those found in samples from HCs. The 2 most promising candidates—miR-146a-5p and miR-221-3p —were further analyzed in the 20 PTC patients mentioned earlier. Serum levels of both miRNAs after 1 to 2 years of follow-up were consistent with ATA responses to therapy in all patients, including 2 with structural evidence of disease whose Tg assays remained negative (<1 ng/mL). CONCLUSION: miR-146a-5p and miR-221-3p hold remarkable promise as serum biomarkers for post-treatment monitoring of PTC patients, especially when Tg assay results are uninformative. Endocrine Society 2017-01-12 /pmc/articles/PMC5677215/ /pubmed/29264441 http://dx.doi.org/10.1210/js.2016-1032 Text en Copyright © 2017 by the Endocrine Society https://creativecommons.org/licenses/by-nc-nd/4.0/ This article has been published under the terms of the Creative Commons Attribution License (CC BY-NC-ND; https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Rosignolo, Francesca Sponziello, Marialuisa Giacomelli, Laura Russo, Diego Pecce, Valeria Biffoni, Marco Bellantone, Rocco Lombardi, Celestino Pio Lamartina, Livia Grani, Giorgio Durante, Cosimo Filetti, Sebastiano Verrienti, Antonella Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up |
title | Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up |
title_full | Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up |
title_fullStr | Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up |
title_full_unstemmed | Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up |
title_short | Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up |
title_sort | identification of thyroid-associated serum microrna profiles and their potential use in thyroid cancer follow-up |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677215/ https://www.ncbi.nlm.nih.gov/pubmed/29264441 http://dx.doi.org/10.1210/js.2016-1032 |
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