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Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicologica...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677299/ https://www.ncbi.nlm.nih.gov/pubmed/29138564 http://dx.doi.org/10.2147/IJN.S149070 |
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author | Liu, Jia Kang, Yiyuan Yin, Suhan Song, Bin Wei, Limin Chen, Liangjiao Shao, Longquan |
author_facet | Liu, Jia Kang, Yiyuan Yin, Suhan Song, Bin Wei, Limin Chen, Liangjiao Shao, Longquan |
author_sort | Liu, Jia |
collection | PubMed |
description | Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicological profile of ZnO NPs in the immune system, digestive system, however, information regarding the toxicity of ZnO NPs in the nervous system is scarce. In this study, we detected the cytotoxicity of two types of ZnO NPs of various sizes – ZnO(a) NPs and ZnO(b) NPs – and we characterized the shedding ability of zinc ions within culture medium and the cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnO(b) NPs induced more significant toxic responses than ZnO(a) NPs in SHSY5Y cells. In conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent manner, which can probably be attributed to their ion-shedding ability. |
format | Online Article Text |
id | pubmed-5677299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-56772992017-11-14 Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner Liu, Jia Kang, Yiyuan Yin, Suhan Song, Bin Wei, Limin Chen, Liangjiao Shao, Longquan Int J Nanomedicine Original Research Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicological profile of ZnO NPs in the immune system, digestive system, however, information regarding the toxicity of ZnO NPs in the nervous system is scarce. In this study, we detected the cytotoxicity of two types of ZnO NPs of various sizes – ZnO(a) NPs and ZnO(b) NPs – and we characterized the shedding ability of zinc ions within culture medium and the cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnO(b) NPs induced more significant toxic responses than ZnO(a) NPs in SHSY5Y cells. In conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent manner, which can probably be attributed to their ion-shedding ability. Dove Medical Press 2017-11-01 /pmc/articles/PMC5677299/ /pubmed/29138564 http://dx.doi.org/10.2147/IJN.S149070 Text en © 2017 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liu, Jia Kang, Yiyuan Yin, Suhan Song, Bin Wei, Limin Chen, Liangjiao Shao, Longquan Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner |
title | Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner |
title_full | Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner |
title_fullStr | Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner |
title_full_unstemmed | Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner |
title_short | Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner |
title_sort | zinc oxide nanoparticles induce toxic responses in human neuroblastoma shsy5y cells in a size-dependent manner |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677299/ https://www.ncbi.nlm.nih.gov/pubmed/29138564 http://dx.doi.org/10.2147/IJN.S149070 |
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