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A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer

A light-up fluorescent probe for the detection of adenosine was constructed with an AIE (aggregation-induced emission) molecule and a DNA aptamer. The AIE molecule was used as a signal generator, and the DNA aptamer was used as a recognition element for adenosine. The emission of the AIE molecule wa...

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Autores principales: Hu, Yingying, Liu, Jingjing, You, Xiangyu, Wang, Can, Li, Zhen, Xie, Weihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677307/
https://www.ncbi.nlm.nih.gov/pubmed/28961223
http://dx.doi.org/10.3390/s17102246
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author Hu, Yingying
Liu, Jingjing
You, Xiangyu
Wang, Can
Li, Zhen
Xie, Weihong
author_facet Hu, Yingying
Liu, Jingjing
You, Xiangyu
Wang, Can
Li, Zhen
Xie, Weihong
author_sort Hu, Yingying
collection PubMed
description A light-up fluorescent probe for the detection of adenosine was constructed with an AIE (aggregation-induced emission) molecule and a DNA aptamer. The AIE molecule was used as a signal generator, and the DNA aptamer was used as a recognition element for adenosine. The emission of the AIE molecule was due to its intramolecular rotation restriction induced by the aptamer upon binding of adenosine. The optimal component ratio of the probe was AIE molecule/DNA aptamer = 100 (μM/μM). The calibration curve of adenosine detection showed a linear range of 10 pM to 0.5 μM with an R(2) of 0.996, and the detection limit of the probe was 10 pM. The probe exhibited a good selectivity to adenosine against its analogs (uridine, guanosine, and cytidine). The probe was used to detect adenosine in urine samples, a recovery from 86.8% to 90.0% for the spiked concentrations of adenosine (0.01, 0.05, 0.1 μM). The relative standard deviation from 1.2% to 2.0% was obtained. The intra-day and inter-day tests also showed good precisions, with measurement RSD values of 2.3% and 2.1%, respectively.
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spelling pubmed-56773072017-11-17 A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer Hu, Yingying Liu, Jingjing You, Xiangyu Wang, Can Li, Zhen Xie, Weihong Sensors (Basel) Article A light-up fluorescent probe for the detection of adenosine was constructed with an AIE (aggregation-induced emission) molecule and a DNA aptamer. The AIE molecule was used as a signal generator, and the DNA aptamer was used as a recognition element for adenosine. The emission of the AIE molecule was due to its intramolecular rotation restriction induced by the aptamer upon binding of adenosine. The optimal component ratio of the probe was AIE molecule/DNA aptamer = 100 (μM/μM). The calibration curve of adenosine detection showed a linear range of 10 pM to 0.5 μM with an R(2) of 0.996, and the detection limit of the probe was 10 pM. The probe exhibited a good selectivity to adenosine against its analogs (uridine, guanosine, and cytidine). The probe was used to detect adenosine in urine samples, a recovery from 86.8% to 90.0% for the spiked concentrations of adenosine (0.01, 0.05, 0.1 μM). The relative standard deviation from 1.2% to 2.0% was obtained. The intra-day and inter-day tests also showed good precisions, with measurement RSD values of 2.3% and 2.1%, respectively. MDPI 2017-09-29 /pmc/articles/PMC5677307/ /pubmed/28961223 http://dx.doi.org/10.3390/s17102246 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Yingying
Liu, Jingjing
You, Xiangyu
Wang, Can
Li, Zhen
Xie, Weihong
A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer
title A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer
title_full A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer
title_fullStr A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer
title_full_unstemmed A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer
title_short A Light-Up Probe for Detection of Adenosine in Urine Samples by a Combination of an AIE Molecule and an Aptamer
title_sort light-up probe for detection of adenosine in urine samples by a combination of an aie molecule and an aptamer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5677307/
https://www.ncbi.nlm.nih.gov/pubmed/28961223
http://dx.doi.org/10.3390/s17102246
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