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Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin
OBJECTIVES: The earwax (cerumen) that covers external auditory canal (EAC) skin contains a mixture of ceruminous and sebaceous gland substances, such as lipids, peptides, and proteins. The components secreted from the ceruminous gland that is a modified sweat gland form cerumen and contain several a...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678038/ https://www.ncbi.nlm.nih.gov/pubmed/28866875 http://dx.doi.org/10.21053/ceo.2017.00129 |
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author | Yoon, Yong Joo Yoon, Jin Lee, Eun Jung Kim, Jong Seung |
author_facet | Yoon, Yong Joo Yoon, Jin Lee, Eun Jung Kim, Jong Seung |
author_sort | Yoon, Yong Joo |
collection | PubMed |
description | OBJECTIVES: The earwax (cerumen) that covers external auditory canal (EAC) skin contains a mixture of ceruminous and sebaceous gland substances, such as lipids, peptides, and proteins. The components secreted from the ceruminous gland that is a modified sweat gland form cerumen and contain several antimicrobial factors. Since substance P (SP) and calcitonin gene-related peptide (CGRP), known as a secretagogue, have been found in sweat glands, our purpose was to determine the expression of SP and CGRP in the glands of EAC skin. METHODS: Sections of normal human EAC skins were immunostained for the presence of SP and CGRP using polyclonal antibodies. Immunoreactivity was detected using an avidin-biotin peroxidase complex method. RESULTS: SP staining was found in ceruminous gland acini and myoepithelial cells. But the SP staining was not found in the sebaceous glands and epidermal region. CGRP was strongly stained in the ceruminous gland and weakly in the sebaceous gland cells. Interestingly, most prominent staining of SP and CGRP was noted in the myoepithelial cells of the ceruminous gland. CONCLUSION: The findings in this study suggest that SP and CGRP are expressed in the glands of the EAC skin and secreted in the process of ceruminous gland secretion. |
format | Online Article Text |
id | pubmed-5678038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-56780382017-12-01 Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin Yoon, Yong Joo Yoon, Jin Lee, Eun Jung Kim, Jong Seung Clin Exp Otorhinolaryngol Original Article OBJECTIVES: The earwax (cerumen) that covers external auditory canal (EAC) skin contains a mixture of ceruminous and sebaceous gland substances, such as lipids, peptides, and proteins. The components secreted from the ceruminous gland that is a modified sweat gland form cerumen and contain several antimicrobial factors. Since substance P (SP) and calcitonin gene-related peptide (CGRP), known as a secretagogue, have been found in sweat glands, our purpose was to determine the expression of SP and CGRP in the glands of EAC skin. METHODS: Sections of normal human EAC skins were immunostained for the presence of SP and CGRP using polyclonal antibodies. Immunoreactivity was detected using an avidin-biotin peroxidase complex method. RESULTS: SP staining was found in ceruminous gland acini and myoepithelial cells. But the SP staining was not found in the sebaceous glands and epidermal region. CGRP was strongly stained in the ceruminous gland and weakly in the sebaceous gland cells. Interestingly, most prominent staining of SP and CGRP was noted in the myoepithelial cells of the ceruminous gland. CONCLUSION: The findings in this study suggest that SP and CGRP are expressed in the glands of the EAC skin and secreted in the process of ceruminous gland secretion. Korean Society of Otorhinolaryngology-Head and Neck Surgery 2017-12 2017-09-05 /pmc/articles/PMC5678038/ /pubmed/28866875 http://dx.doi.org/10.21053/ceo.2017.00129 Text en Copyright © 2017 by Korean Society of Otorhinolaryngology-Head and Neck Surgery This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yoon, Yong Joo Yoon, Jin Lee, Eun Jung Kim, Jong Seung Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin |
title | Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin |
title_full | Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin |
title_fullStr | Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin |
title_full_unstemmed | Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin |
title_short | Substance P and Calcitonin Gene-Related Peptide in the Glands of External Auditory Canal Skin |
title_sort | substance p and calcitonin gene-related peptide in the glands of external auditory canal skin |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678038/ https://www.ncbi.nlm.nih.gov/pubmed/28866875 http://dx.doi.org/10.21053/ceo.2017.00129 |
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