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PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923

OBJECTIVE: ATCC25923 is a Staphylococcus aureus strain that is positive for the Panton Valentin leukocidin. It has been used for decades as reference strain. We observed that two separately maintained clones of ATCC25923 (“G477 and G478”) differed grossly in the expression of this toxin. For that re...

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Autores principales: Stieber, Bettina, Sabat, Artur, Monecke, Stefan, Slickers, Peter, Akkerboom, Viktoria, Müller, Elke, Friedrich, Alexander W., Ehricht, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678758/
https://www.ncbi.nlm.nih.gov/pubmed/29115975
http://dx.doi.org/10.1186/s13104-017-2891-3
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author Stieber, Bettina
Sabat, Artur
Monecke, Stefan
Slickers, Peter
Akkerboom, Viktoria
Müller, Elke
Friedrich, Alexander W.
Ehricht, Ralf
author_facet Stieber, Bettina
Sabat, Artur
Monecke, Stefan
Slickers, Peter
Akkerboom, Viktoria
Müller, Elke
Friedrich, Alexander W.
Ehricht, Ralf
author_sort Stieber, Bettina
collection PubMed
description OBJECTIVE: ATCC25923 is a Staphylococcus aureus strain that is positive for the Panton Valentin leukocidin. It has been used for decades as reference strain. We observed that two separately maintained clones of ATCC25923 (“G477 and G478”) differed grossly in the expression of this toxin. For that reason, both clones were sequenced using an Illumina MiSeq instrument. After assembling, the final sequences were analyzed and mapped to a previously published ATCC25923 sequence (GenBank CP009361) using bl2seq from the NCBI Blast2 package. RESULTS: The genomes of G477 and G478 size 2778,859 and 2792,213 nucleotides, respectively. Both genomes include a circular plasmid of 27,490 nucleotides. The sequence of the G477 chromosome maps nearly exactly to CP009361. G478 has a slightly larger size because of the presence of an additional transposable element tnp13k. The second copy of that tnp13k element is located in an intergenic region between the genes mazF and rsbU. The sequences of the ATCC25923 clones G477 and G478 differ mainly in the insertion of a second tnp13k element between the genes mazF and rsbU. That insertion may lead to a different transcription of that genome region resulting in upregulation of the expression of the Panton-Valentine leukocidin in the ATCC25923 clone G478. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-017-2891-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-56787582017-11-17 PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923 Stieber, Bettina Sabat, Artur Monecke, Stefan Slickers, Peter Akkerboom, Viktoria Müller, Elke Friedrich, Alexander W. Ehricht, Ralf BMC Res Notes Research Note OBJECTIVE: ATCC25923 is a Staphylococcus aureus strain that is positive for the Panton Valentin leukocidin. It has been used for decades as reference strain. We observed that two separately maintained clones of ATCC25923 (“G477 and G478”) differed grossly in the expression of this toxin. For that reason, both clones were sequenced using an Illumina MiSeq instrument. After assembling, the final sequences were analyzed and mapped to a previously published ATCC25923 sequence (GenBank CP009361) using bl2seq from the NCBI Blast2 package. RESULTS: The genomes of G477 and G478 size 2778,859 and 2792,213 nucleotides, respectively. Both genomes include a circular plasmid of 27,490 nucleotides. The sequence of the G477 chromosome maps nearly exactly to CP009361. G478 has a slightly larger size because of the presence of an additional transposable element tnp13k. The second copy of that tnp13k element is located in an intergenic region between the genes mazF and rsbU. The sequences of the ATCC25923 clones G477 and G478 differ mainly in the insertion of a second tnp13k element between the genes mazF and rsbU. That insertion may lead to a different transcription of that genome region resulting in upregulation of the expression of the Panton-Valentine leukocidin in the ATCC25923 clone G478. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-017-2891-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-07 /pmc/articles/PMC5678758/ /pubmed/29115975 http://dx.doi.org/10.1186/s13104-017-2891-3 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Stieber, Bettina
Sabat, Artur
Monecke, Stefan
Slickers, Peter
Akkerboom, Viktoria
Müller, Elke
Friedrich, Alexander W.
Ehricht, Ralf
PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923
title PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923
title_full PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923
title_fullStr PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923
title_full_unstemmed PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923
title_short PVL overexpression due to genomic rearrangements and mutations in the S. aureus reference strain ATCC25923
title_sort pvl overexpression due to genomic rearrangements and mutations in the s. aureus reference strain atcc25923
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678758/
https://www.ncbi.nlm.nih.gov/pubmed/29115975
http://dx.doi.org/10.1186/s13104-017-2891-3
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