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Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice
BACKGROUND: Transplantation of neural stem cells (NSCs) has been proposed as a promising therapeutic strategy for the treatment of ischemia/reperfusion (I/R)-induced brain injury. However, existing evidence has also challenged this therapy on its limitations, such as the difficulty for stem cells to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678778/ https://www.ncbi.nlm.nih.gov/pubmed/29115993 http://dx.doi.org/10.1186/s13287-017-0677-0 |
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author | Zhang, Tingting Yang, Xiao Liu, Tengyuan Shao, Jiaxiang Fu, Ningzhen Yan, Aijuan Geng, Keyi Xia, Weiliang |
author_facet | Zhang, Tingting Yang, Xiao Liu, Tengyuan Shao, Jiaxiang Fu, Ningzhen Yan, Aijuan Geng, Keyi Xia, Weiliang |
author_sort | Zhang, Tingting |
collection | PubMed |
description | BACKGROUND: Transplantation of neural stem cells (NSCs) has been proposed as a promising therapeutic strategy for the treatment of ischemia/reperfusion (I/R)-induced brain injury. However, existing evidence has also challenged this therapy on its limitations, such as the difficulty for stem cells to survive after transplantation due to the unfavorable microenvironment in the ischemic brain. Herein, we have investigated whether preconditioning of NSCs with adjudin, a small molecule compound, could enhance their survivability and further improve the therapeutic effect for NSC-based stroke therapy. METHOD: We aimed to examine the effect of adjudin pretreatment on NSCs by measuring a panel of parameters after their transplantation into the infarct area of ipsilateral striatum 24 hours after I/R in mice. RESULTS: We found that pretreatment of NSCs with adjudin could enhance the viability of NSCs after their transplantation into the stroke-induced infarct area. Compared with the untreated NSC group, the adjudin-preconditioned group showed decreased infarct volume and neurobehavioral deficiency through ameliorating blood–brain barrier disruption and promoting the expression and secretion of brain-derived neurotrophic factor. We also employed H(2)O(2)-induced cell death model in vitro and found that adjudin preconditioning could promote NSC survival through inhibition of oxidative stress and activation of Akt signaling pathway. CONCLUSION: This study showed that adjudin could be used to precondition NSCs to enhance their survivability and improve recovery in the stroke model, unveiling the value of adjudin for stem cell-based stroke therapy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0677-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5678778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56787782017-11-17 Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice Zhang, Tingting Yang, Xiao Liu, Tengyuan Shao, Jiaxiang Fu, Ningzhen Yan, Aijuan Geng, Keyi Xia, Weiliang Stem Cell Res Ther Research BACKGROUND: Transplantation of neural stem cells (NSCs) has been proposed as a promising therapeutic strategy for the treatment of ischemia/reperfusion (I/R)-induced brain injury. However, existing evidence has also challenged this therapy on its limitations, such as the difficulty for stem cells to survive after transplantation due to the unfavorable microenvironment in the ischemic brain. Herein, we have investigated whether preconditioning of NSCs with adjudin, a small molecule compound, could enhance their survivability and further improve the therapeutic effect for NSC-based stroke therapy. METHOD: We aimed to examine the effect of adjudin pretreatment on NSCs by measuring a panel of parameters after their transplantation into the infarct area of ipsilateral striatum 24 hours after I/R in mice. RESULTS: We found that pretreatment of NSCs with adjudin could enhance the viability of NSCs after their transplantation into the stroke-induced infarct area. Compared with the untreated NSC group, the adjudin-preconditioned group showed decreased infarct volume and neurobehavioral deficiency through ameliorating blood–brain barrier disruption and promoting the expression and secretion of brain-derived neurotrophic factor. We also employed H(2)O(2)-induced cell death model in vitro and found that adjudin preconditioning could promote NSC survival through inhibition of oxidative stress and activation of Akt signaling pathway. CONCLUSION: This study showed that adjudin could be used to precondition NSCs to enhance their survivability and improve recovery in the stroke model, unveiling the value of adjudin for stem cell-based stroke therapy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0677-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-07 /pmc/articles/PMC5678778/ /pubmed/29115993 http://dx.doi.org/10.1186/s13287-017-0677-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhang, Tingting Yang, Xiao Liu, Tengyuan Shao, Jiaxiang Fu, Ningzhen Yan, Aijuan Geng, Keyi Xia, Weiliang Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
title | Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
title_full | Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
title_fullStr | Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
title_full_unstemmed | Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
title_short | Adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
title_sort | adjudin-preconditioned neural stem cells enhance neuroprotection after ischemia reperfusion in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678778/ https://www.ncbi.nlm.nih.gov/pubmed/29115993 http://dx.doi.org/10.1186/s13287-017-0677-0 |
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